Label-free imaging of drug distribution and metabolism in colon cancer cells by Raman microscopy

The Analyst ◽  
2014 ◽  
Vol 139 (5) ◽  
pp. 1155 ◽  
Author(s):  
Samir F. El-Mashtoly ◽  
Dennis Petersen ◽  
Hesham K. Yosef ◽  
Axel Mosig ◽  
Anke Reinacher-Schick ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Drug Distribution ◽  
Raman Microscopy ◽  
Label Free ◽  
Colon Cancer Cells

Exploring the efficacy and cellular uptake of sorafenib in colon cancer cells by Raman micro-spectroscopy

The Analyst ◽  
2018 ◽  
Vol 143 (24) ◽  
pp. 6069-6078 ◽  
Author(s):  
H. K. Yosef ◽  
T. Frick ◽  
M. K. Hammoud ◽  
A. Maghnouj ◽  
S. Hahn ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Cellular Uptake ◽  
Raman Microscopy ◽  
Label Free ◽  
Colon Cancer Cells

This study demonstrates the efficacy and distribution of sorafenib in colon cancer cells by label-free Raman microscopy.

Label-free distinction between p53+/+ and p53 -/- colon cancer cells using a graphene based SERS platform

2018 ◽  
Vol 118 ◽  
pp. 108-114 ◽  
Author(s):  
Owen Liang ◽  
Pu Wang ◽  
Ming Xia ◽  
Catherine Augello ◽  
Fan Yang ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Label Free ◽  
Colon Cancer Cells

Label-free quantitative proteomic profiling of colon cancer cells identifies acetyl-CoA carboxylase alpha as antitumor target of Citrus limon-derived nanovesicles

2018 ◽  
Vol 173 ◽  
pp. 1-11 ◽  
Author(s):  
Stefania Raimondo ◽  
Laura Saieva ◽  
Marta Cristaldi ◽  
Francesca Monteleone ◽  
Simona Fontana ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Citrus Limon ◽  
Acetyl Coa Carboxylase ◽  
Label Free ◽  
Colon Cancer Cells ◽  
Proteomic Profiling ◽  
Acetyl Coa

Leptin is a growth factor for human colon cancer cells

2001 ◽  
Vol 120 (5) ◽  
pp. A493-A493
Author(s):  
J HARDWICK ◽  
G VANDENBRINK ◽  
S VANDEVENTER ◽  
M PEPPELENBOSCH
Keyword(s):  
Colon Cancer ◽  
Growth Factor ◽  
Cancer Cells ◽  
Human Colon ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
Human Colon Cancer Cells

Silencing of a death associated protein kinase as a pro-survival factor in colon cancer cells

Endoscopy ◽  
2005 ◽  
Vol 37 (05) ◽  
Author(s):  
GA Doherty ◽  
SM Byrne ◽  
SC Austin ◽  
GM Scully ◽  
EW Kay ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Protein Kinase ◽  
Cancer Cells ◽  
Colon Cancer Cells ◽  
Survival Factor ◽  
Death Associated Protein Kinase

Cytotoxic Effect of the Combination of Gemcitabine and Atorvastatin Loaded in Microemulsion on the HCT116 Colon Cancer Cells

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Mayson H. Alkhatib ◽  
Dalal Al-Saedi ◽  
Wadiah S. Backer
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Anticancer Drugs ◽  
Therapeutic Potential ◽  
Morphological Changes ◽  
In Vitro Study ◽  
Colon Cancer Cells ◽  
Apoptosis Detection ◽  
Hct116 Cells

The combination of anticancer drugs in nanoparticles has great potential as a promising strategy to maximize efficacies by eradicating resistant, reduce the dosage of the drug and minimize toxicities on the normal cells. Gemcitabine (GEM), a nucleoside analogue, and atorvastatin (ATV), a cholesterol lowering agent, have shown anticancer effect with some limitations. The objective of this in vitro study was to evaluate the antitumor activity of the combination therapy of GEM and ATVencapsulated in a microemulsion (ME) formulation in the HCT116 colon cancer cells. The cytotoxicity and efficacy of the formulation were assessed by the 3- (4,5dimethylthiazole-2-yl)-2,5-diphyneltetrazolium bromide (MTT) assay. The mechanism of cell death was examined by observing the morphological changes of treated cells under light microscope, identifying apoptosis by using the ApopNexin apoptosis detection kit, and viewing the morphological changes in the chromatin structure stained with 4′,6-diamidino-2-phenylindole (DAPI) under the inverted fluorescence microscope. It has been found that reducing the concentration of GEM loaded on ME (GEM-ME) from 5μM to 1.67μM by combining it with 3.33μM of ATV in a ME formulation (GEM/2ATV-ME) has preserved the strong cytotoxicity of GEM-ME against HCT116 cells. The current study proved that formulating GEM with ATV in ME has improved the therapeutic potential of GEM and ATV as anticancer drugs.

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