Peptide nucleic acids containing adenine or guanine recognize thymine and cytosine in complementary DNA sequences

1993 ◽  
pp. 800 ◽  
Author(s):  
Michael Egholm ◽  
Carsten Behrens ◽  
Leif Christensen ◽  
Rolf H. Berg ◽  
Peter E. Nielsen ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Dna Sequences ◽  
Peptide Nucleic Acids ◽  
Complementary Dna

scholarly journals The Use of Peptide Nucleic Acids for In Situ Identification of Human Chromosomes

2005 ◽  
Vol 53 (3) ◽  
pp. 395-400 ◽  
Author(s):  
Franck Pellestor ◽  
Petra Paulasova ◽  
Milan Macek ◽  
Samir Hamamah
Keyword(s):  
Nucleic Acids ◽  
Dna Sequences ◽  
Genome Mapping ◽  
Peptide Nucleic Acids ◽  
Human Lymphocytes ◽  
Human Chromosomes ◽  
Great Resistance ◽  
Diagnostic Assays ◽  
New Class

The peptide nucleic acids (PNAs) constitute a remarkable new class of synthetic nucleic acid analogues, based on their peptide-like backbone. This structure gives to PNAs the capacity to hybridize with high affinity and specificity to complementary RNA and DNA sequences and a great resistance to nucleases and proteinases. Originally conceived as ligands for the study of double-stranded DNA, the unique physicochemical properties of PNAs have led to the development of a large variety of research and diagnostic assays, including antigene and antisense therapy, genome mapping, and mutation detection. Over the past few years, PNAs have been shown to be powerful tools in cytogenetics for the rapid in situ identification of human chromosomes and the detection of aneuploidies. Recent studies have reported the successful use of chromosome-specific PNA probes on human lymphocytes, amniocytes, and spermatozoa, as well as on isolated oocytes and blastomeres. Multicolor PNA protocols have been described for the identification of several human chromosomes, indicating that PNAs could become a powerful complement to FISH for in situ chromosomal investigation.

New hetero-oligomeric peptide nucleic acids with improved binding properties to complementary DNA

1997 ◽  
Vol 7 (6) ◽  
pp. 687-690 ◽  
Author(s):  
Stephan Jordan ◽  
Christoph Schwemler ◽  
Winfried Kosch ◽  
Axel Kretschmer ◽  
Udo Stropp ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Peptide Nucleic Acids ◽  
Binding Properties ◽  
Complementary Dna ◽  
Oligomeric Peptide

scholarly journals Fluorescent Biaryl Uracils with C5-Dihydro- and Quinazolinone Heterocyclic Appendages in PNA

Molecules ◽  
2020 ◽  
Vol 25 (8) ◽  
pp. 1995 ◽  
Author(s):  
Ali Heidari ◽  
Arash Ghorbani-Choghamarani ◽  
Maryam Hajjami ◽  
Robert H. E. Hudson
Keyword(s):  
Nucleic Acids ◽  
Dna Sequences ◽  
Photophysical Properties ◽  
Molar Absorptivity ◽  
Transition Metal Catalysts ◽  
Quantum Yields ◽  
Molecular Rotor ◽  
Copper Salts ◽  
Complementary Dna ◽  
Modified Nucleobases

There has been much effort to exploit fluorescence techniques in the detection of nucleic acids. Canonical nucleic acids are essentially nonfluorescent; however, the modification of the nucleobase has proved to be a fruitful way to engender fluorescence. Much of the chemistry used to prepare modified nucleobases relies on expensive transition metal catalysts. In this work, we describe the synthesis of biaryl quinazolinone-uracil nucleobase analogs prepared by the condensation of anthranilamide derivatives and 5-formyluracil using inexpensive copper salts. A selection of modified nucleobases were prepared, and the effect of methoxy- or nitro- group substitution on the photophysical properties was examined. Both the dihydroquinazolinone and quinazolinone modified uracils have much larger molar absorptivity (~4–8×) than natural uracil and produce modest blue fluorescence. The quinazolinone-modified uracils display higher quantum yields than the corresponding dihydroquinazolinones and also show temperature and viscosity dependent emission consistent with molecular rotor behavior. Peptide nucleic acid (PNA) monomers possessing quinazolinone modified uracils were prepared and incorporated into oligomers. In the sequence context examined, the nitro-substituted, methoxy-substituted and unmodified quinazolinone inserts resulted in a stabilization (∆Tm = +4.0/insert; +2.0/insert; +1.0/insert, respectively) relative to control PNA sequence upon hybridization to complementary DNA. All three derivatives responded to hybridization by the “turn-on” of fluorescence intensity by ca. 3-to-4 fold and may find use as probes for complementary DNA sequences.

Molecular insights on the dynamic stability of peptide nucleic acid functionalized carbon and boron nitride nanotubes

2021 ◽  
Vol 23 (1) ◽  
pp. 219-228
Author(s):  
Nabanita Saikia ◽  
Mohamed Taha ◽  
Ravindra Pandey
Keyword(s):  
Nucleic Acid ◽  
Boron Nitride ◽  
Nucleic Acids ◽  
Dynamic Stability ◽  
Peptide Nucleic Acid ◽  
Rational Design ◽  
Peptide Nucleic Acids ◽  
Boron Nitride Nanotubes ◽  
Molecular Hybrids ◽  
Single Wall Nanotubes

The rational design of self-assembled nanobio-molecular hybrids of peptide nucleic acids with single-wall nanotubes rely on understanding how biomolecules recognize and mediate intermolecular interactions with the nanomaterial's surface.

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