A new NAD-dependent alcohol dehydrogenase with opposite facial selectivity useful for asymmetric reduction and cofactor regeneration

1990 ◽  
pp. 677 ◽  
Author(s):  
Gwo-Jenn Shen ◽  
Yi-Fong Wang ◽  
Curt Bradshaw ◽  
Chi-Huey Wong
Keyword(s):  
Alcohol Dehydrogenase ◽  
Asymmetric Reduction ◽  
Cofactor Regeneration ◽  
Facial Selectivity

scholarly journals Deep Eutectic Solvents as Smart Cosubstrate in Alcohol Dehydrogenase-Catalyzed Reductions

Catalysts ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1013
Author(s):  
Santiago Nahuel Chanquia ◽  
Lei Huang ◽  
Guadalupe García Liñares ◽  
Pablo Domínguez de María ◽  
Selin Kara
Keyword(s):  
Alcohol Dehydrogenase ◽  
Thermodynamic Equilibrium ◽  
Choline Chloride ◽  
Deep Eutectic Solvents ◽  
Cofactor Regeneration ◽  
The Novel ◽  
Cinnamyl Alcohol ◽  
Maximum Productivity ◽  
Reaction Media ◽  
Reduction Of Ketones

Alcohol dehydrogenase (ADH) catalyzed reductions in deep eutectic solvents (DESs) may become efficient and sustainable alternatives to afford alcohols. This paper successfully explores the ADH-catalyzed reduction of ketones and aldehydes in a DES composed of choline chloride and 1,4-butanediol, in combination with buffer (Tris-HCl, 20% v/v). 1,4-butanediol (a DES component), acts as a smart cosubstrate for the enzymatic cofactor regeneration, shifting the thermodynamic equilibrium to the product side. By means of the novel DES media, cyclohexanone reduction was optimized to yield maximum productivity with low enzyme amounts (in the range of 10 g L−1 d−1). Notably, with the herein developed reaction media, cinnamaldehyde was reduced to cinnamyl alcohol, an important compound for the fragrance industry, with promising high productivities of ~75 g L−1 d−1.

Efficient asymmetric synthesis of chiral alcohols using high 2-propanol tolerance alcohol dehydrogenase SmADH2 via an environmentally friendly TBCR system

2020 ◽  
Vol 10 (1) ◽  
pp. 70-78 ◽  
Author(s):  
Zeyu Yang ◽  
Hengwei Fu ◽  
Wenjie Ye ◽  
Youyu Xie ◽  
Qinghai Liu ◽  
...  
Keyword(s):  
Alcohol Dehydrogenase ◽  
Asymmetric Synthesis ◽  
Environmentally Friendly ◽  
Cofactor Regeneration ◽  
Chiral Alcohols ◽  
Regeneration System ◽  
High Substrate ◽  
System A ◽  
System P

Based on substrate-coupled cofactor regeneration system, a high 2-propanol tolerance SmADH2 together with TBCR system can synthesise structurally diverse chiral alcohols at a high substrate loading with only 1.25 equivalents of 2-propanol.

Sign in / Sign up

Export Citation Format

Share Document