High-sensitivity in vivo imaging for tumors using a spectral up-conversion nanoparticle NaYF4: Yb3+, Er3+ in cooperation with a microtubulin inhibitor

Nanoscale ◽  
2012 ◽  
Vol 4 (13) ◽  
pp. 3901 ◽  
Author(s):  
Yanchun Wei ◽  
Qun Chen ◽  
Baoyan Wu ◽  
Aiguo Zhou ◽  
Da Xing
Keyword(s):  
In Vivo Imaging ◽  
High Sensitivity

Mitochondria-targeted colorimetric and fluorescent probes for hypochlorite and their applications for in vivo imaging

2014 ◽  
Vol 50 (63) ◽  
pp. 8640-8643 ◽  
Author(s):  
Ji-Ting Hou ◽  
Ming-Yu Wu ◽  
Kun Li ◽  
Jin Yang ◽  
Kang-Kang Yu ◽  
...  
Keyword(s):  
Response Time ◽  
Fluorescent Probes ◽  
In Vivo Imaging ◽  
High Sensitivity ◽  
Rapid Response ◽  
Excellent Selectivity

Two probes that exhibited high sensitivity and excellent selectivity toward ClO−among ROS with a rapid response time were presented, which could selectively detect ClO−in the mitochondria of the cells.

scholarly journals High Sensitivity In Vivo Imaging of Cancer Metastasis Using a Near-Infrared Luciferin Analogue seMpai

2020 ◽  
Vol 21 (21) ◽  
pp. 7896
Author(s):  
Jun Nakayama ◽  
Ryohei Saito ◽  
Yusuke Hayashi ◽  
Nobuo Kitada ◽  
Shota Tamaki ◽  
...  
Keyword(s):  
Single Cell ◽  
In Vivo Imaging ◽  
Cancer Metastasis ◽  
Near Infrared ◽  
High Sensitivity ◽  
Single Cell Level ◽  
Background Signal ◽  
Cell Level ◽  
Highly Sensitive

Bioluminescence imaging (BLI) is useful to monitor cell movement and gene expression in live animals. However, D-luciferin has a short wavelength (560 nm) which is absorbed by tissues and the use of near-infrared (NIR) luciferin analogues enable high sensitivity in vivo BLI. The AkaLumine-AkaLuc BLI system (Aka-BLI) can detect resolution at the single-cell level; however, it has a clear hepatic background signal. Here, to enable the highly sensitive detection of bioluminescence from the surrounding liver tissues, we focused on seMpai (C15H16N3O2S) which has been synthesized as a luciferin analogue and has high luminescent abilities as same as AkaLumine. We demonstrated that seMpai BLI could detect micro-signals near the liver without any background signal. The solution of seMpai was neutral; therefore, seMpai imaging did not cause any adverse effect in mice. seMpai enabled a highly sensitive in vivo BLI as compared to previous techniques. Our findings suggest that the development of a novel mutated luciferase against seMpai may enable a highly sensitive BLI at the single-cell level without any background signal. Novel seMpai BLI system can be used for in vivo imaging in the fields of life sciences and medicine.

In vivo imaging of hepatocellular nitric oxide using a hepatocyte-targeting fluorescent sensor

2018 ◽  
Vol 54 (52) ◽  
pp. 7231-7234 ◽  
Author(s):  
Peisheng Zhang ◽  
Yong Tian ◽  
Hui Liu ◽  
Junyu Ren ◽  
Hong Wang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
In Vivo Imaging ◽  
Water Solubility ◽  
Fluorescent Sensor ◽  
High Sensitivity ◽  
No Sensor ◽  
Excellent Selectivity

A hepatocyte-targeting fluorescent NO sensor has been fabricated with good water solubility, excellent selectivity, and high sensitivity (∼1.62 nM).

scholarly journals How to Select Firefly Luciferin Analogues for In Vivo Imaging

2021 ◽  
Vol 22 (4) ◽  
pp. 1848
Author(s):  
Ryohei Saito-Moriya ◽  
Jun Nakayama ◽  
Genta Kamiya ◽  
Nobuo Kitada ◽  
Rika Obata ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Life Science ◽  
Bioluminescence Imaging ◽  
High Sensitivity ◽  
The Novel ◽  
Current Review ◽  
Firefly Luciferin ◽  
In Vivo Bioluminescence Imaging ◽  
Luciferase Enzyme

Bioluminescence reactions are widely applied in optical in vivo imaging in the life science and medical fields. Such reactions produce light upon the oxidation of a luciferin (substrate) catalyzed by a luciferase (enzyme), and this bioluminescence enables the quantification of tumor cells and gene expression in animal models. Many researchers have developed single-color or multicolor bioluminescence systems based on artificial luciferin analogues and/or luciferase mutants, for application in vivo bioluminescence imaging (BLI). In the current review, we focus on the characteristics of firefly BLI technology and discuss the development of luciferin analogues for high-resolution in vivo BLI. In addition, we discuss the novel luciferin analogues TokeOni and seMpai, which show potential as high-sensitivity in vivo BLI reagents.

Fluorescent proteins for in vivo imaging, where's the biliverdin?

2020 ◽  
Vol 48 (6) ◽  
pp. 2657-2667
Author(s):  
Felipe Montecinos-Franjola ◽  
John Y. Lin ◽  
Erik A. Rodriguez
Keyword(s):  
Hydrogen Peroxide ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Fluorescent Imaging ◽  
Infrared Light ◽  
Red Fluorescent Protein ◽  
Color Palette

Noninvasive fluorescent imaging requires far-red and near-infrared fluorescent proteins for deeper imaging. Near-infrared light penetrates biological tissue with blood vessels due to low absorbance, scattering, and reflection of light and has a greater signal-to-noise due to less autofluorescence. Far-red and near-infrared fluorescent proteins absorb light >600 nm to expand the color palette for imaging multiple biosensors and noninvasive in vivo imaging. The ideal fluorescent proteins are bright, photobleach minimally, express well in the desired cells, do not oligomerize, and generate or incorporate exogenous fluorophores efficiently. Coral-derived red fluorescent proteins require oxygen for fluorophore formation and release two hydrogen peroxide molecules. New fluorescent proteins based on phytochrome and phycobiliproteins use biliverdin IXα as fluorophores, do not require oxygen for maturation to image anaerobic organisms and tumor core, and do not generate hydrogen peroxide. The small Ultra-Red Fluorescent Protein (smURFP) was evolved from a cyanobacterial phycobiliprotein to covalently attach biliverdin as an exogenous fluorophore. The small Ultra-Red Fluorescent Protein is biophysically as bright as the enhanced green fluorescent protein, is exceptionally photostable, used for biosensor development, and visible in living mice. Novel applications of smURFP include in vitro protein diagnostics with attomolar (10−18 M) sensitivity, encapsulation in viral particles, and fluorescent protein nanoparticles. However, the availability of biliverdin limits the fluorescence of biliverdin-attaching fluorescent proteins; hence, extra biliverdin is needed to enhance brightness. New methods for improved biliverdin bioavailability are necessary to develop improved bright far-red and near-infrared fluorescent proteins for noninvasive imaging in vivo.

In vivo imaging of beta-amyloid load in transgenic rodents with [F-18]FDDNP microPET

2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S588-S588
Author(s):  
Vladimir Kepe ◽  
Gregory M Cole ◽  
Jie Liu ◽  
Dorothy G Flood ◽  
Stephen P Trusko ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Beta Amyloid ◽  
Amyloid Load

In vivo imaging of liver injury and regeneration by functional two-photon microscopy

2016 ◽  
Vol 54 (12) ◽  
pp. 1343-1404
Author(s):  
A Ghallab ◽  
R Reif ◽  
R Hassan ◽  
AS Seddek ◽  
JG Hengstler
Keyword(s):  
Liver Injury ◽  
In Vivo Imaging ◽  
Two Photon Microscopy ◽  
Two Photon
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