scholarly journals Time encoded multicolor fluorescence detection in a microfluidic flow cytometer

Lab on a Chip ◽  
2012 ◽  
Vol 12 (23) ◽  
pp. 5057 ◽  
Author(s):  
Joerg Martini ◽  
Michael I. Recht ◽  
Malte Huck ◽  
Marshall W. Bern ◽  
Noble M. Johnson ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Flow Cytometer ◽  
Microfluidic Flow ◽  
Multicolor Fluorescence

Microfluidic Flow Cytometer for Multiparameter Screening of Fluorophore Photophysics

CLEO: 2014 ◽  
2014 ◽  
Author(s):  
Kevin M. Dean ◽  
Lloyd M. Davis ◽  
Jennifer L. Lubbeck ◽  
Premashis Manna ◽  
Amy E. Palmer ◽  
...  
Keyword(s):  
Flow Cytometer ◽  
Microfluidic Flow

scholarly journals Full-wafer in-situ fabrication and packaging of microfluidic flow cytometer with photo-patternable adhesive polymers

2017 ◽  
Vol 20 (1) ◽  
Author(s):  
Koen de Wijs ◽  
Chengxun Liu ◽  
Bivragh Majeed ◽  
Karolien Jans ◽  
John M. O’Callaghan ◽  
...  
Keyword(s):  
Flow Cytometer ◽  
In Situ Fabrication ◽  
Microfluidic Flow

A portable microfluidic flow cytometer based on simultaneous detection of impedance and fluorescence

2010 ◽  
Vol 25 (6) ◽  
pp. 1509-1515 ◽  
Author(s):  
Segyeong Joo ◽  
Kee Hyun Kim ◽  
Hee Chan Kim ◽  
Taek Dong Chung
Keyword(s):  
Simultaneous Detection ◽  
Flow Cytometer ◽  
Microfluidic Flow

Time-resolved microfluidic flow cytometer for decoding luminescence lifetimes in the microsecond region

Lab on a Chip ◽  
2020 ◽  
Vol 20 (3) ◽  
pp. 655-664
Author(s):  
Yan Wang ◽  
Nima Sayyadi ◽  
Xianlin Zheng ◽  
Travis A. Woods ◽  
Robert C. Leif ◽  
...  
Keyword(s):  
Flow Cytometer ◽  
Time Resolved ◽  
Luminescence Detection ◽  
Microfluidic Flow

Time-resolved luminescence detection using long-lived probes with lifetimes in the microsecond region have shown great potential in ultrasensitive and multiplexed bioanalysis.

Characterization of Microscale Particles Using a Microfluidic Flow Cytometer Equipped With a Multi-Plex Photon Counter

2013 ◽  
Author(s):  
Pouya Asrar ◽  
Nastaran Hashemi
Keyword(s):  
Integrated Optics ◽  
Scattered Light ◽  
Photon Counting ◽  
High Gain ◽  
Flow Cytometer ◽  
Photon Counter ◽  
Microfluidic Flow ◽  
Compact Size ◽  
Using Data ◽  
New Generation

We have shown the design and fabrication of a microfluidic flow cytometer. The microfluidic flow cytometer has been used to characterize microspheres of different sizes. The device is consisted of a microchannel, electronics, and integrated optics. The microchannel has three inlets. Two inlets are used to introduce sheath flows and one middle inlet is assigned as sample inlet. The sample flow is hydrodynamically focused at the center of the microchannel by two side streams (sheath flows). Also arrays of four chevron grooves compress the sample flow from the top and bottom of the microchannel. The core flow contains microspheres at a certain concentration. Detection of the microspheres at the interrogation region of the channel is performed by integrated optics and electronics. The scattered light emitted from the microspheres is collected by a multi-plex photo diode (MPPC). The results are recorded using data acquisition (DAQ) unit. The MPPCs employed in the setup is the new generation of photon counter devices with an excellent detection limit, a compact size, and capability of recording data at high gain compared to previous generation of photodetectors such as photomultipliers or avalanche photon diodes. The flow cytometer was sensitive enough to collect data from 3 μm microspheres using such mentioned sensitive photon counting unit. We have also used COMSOL Multiphysics software to investigate velocity and pressure distribution as well as concentration distribution along the microchannel. The average voltage collected by MPPC was 1.9 V for 10.2 μm and 1.6 V for 3.2 μm microsphere.

scholarly journals Integration of glass micropipettes with a 3D printed aligner for microfluidic flow cytometer

2018 ◽  
Vol 269 ◽  
pp. 382-387 ◽  
Author(s):  
Abdullah Bayram ◽  
Murat Serhatlioglu ◽  
Bulend Ortac ◽  
Serafettin Demic ◽  
Caglar Elbuken ◽  
...  
Keyword(s):  
Flow Cytometer ◽  
Microfluidic Flow ◽  
3D Printed

MICROFLUIDIC FLOW-THROUGH REACTOR WITH ELECTROCHEMICAL SENSOR ARRAY FOR REAL-TIME PCR

2009 ◽  
Vol 23 (03) ◽  
pp. 369-372
Author(s):  
HUEY-FANG TEH ◽  
NAVEEN RAMALINGAM ◽  
HAI-QING GONG ◽  
SWEE-NGIN TAN
Keyword(s):  
Real Time ◽  
Fluorescence Detection ◽  
Microelectrode Array ◽  
Dna Amplification ◽  
Active Species ◽  
Dna Intercalator ◽  
Detection Scheme ◽  
Microfluidic Flow ◽  
Pcr Products ◽  
Flow Through

We developed an integrated microfluidic flow-through EC-PCR (EC-PCR) microdevice for the concurrent DNA amplification, PCR products EC detection and PCR products quantification instead of the current available fluorescence detection scheme. The microfluidic flow-through EC-PCR microdevice was fabricated with the state-of-the-art microfabrication technology, by bonding a bottom glass substrate having a microelectrode array to a top glass cover having the microchannels made of PDMS material. Both the amplification of the target DNA sequence and the subsequent EC detection of the PCR products were carried out concurrently on the integrated device by real-time monitoring. The underlying principle of the microfluidic flow-through EC-PCR method was based on the changes of current signal of methylene blue (MB), which worked as an electrochemically active species DNA intercalator in the PCR mixture, during the amplification process at the extension phase. The results shown in this work indicated that the nucleic acid analysis could be performed in a fast thermal cycling and true real-time quantitative electrochemical detection. The signal variation trends of the EC detection and the fluorescence detection were the same in our verification measurements for both methods, which suggested that the EC detection method was feasible for this application.

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