AOTF-based multicolor fluorescence detection for short tandem repeat (STR) analysis in an electrophoretic microdevice

Lab on a Chip ◽  
2008 ◽  
Vol 8 (8) ◽  
pp. 1285 ◽  
Author(s):  
James M. Karlinsey ◽  
James P. Landers
Keyword(s):  
Fluorescence Detection ◽  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Str Analysis ◽  
Multicolor Fluorescence ◽  
Short Tandem

Evaluation genotypes of cancer cell lines HCC1954 and SiHa by short tandem repeat (STR) analysis and DNA sequencing

2018 ◽  
Vol 45 (6) ◽  
pp. 2689-2695 ◽  
Author(s):  
Jiewen Fu ◽  
Jingliang Cheng ◽  
Xiaoyan Liu ◽  
Jun Li ◽  
Chunli Wei ◽  
...  
Keyword(s):  
Dna Sequencing ◽  
Cell Lines ◽  
Cancer Cell ◽  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Cancer Cell Lines ◽  
Str Analysis ◽  
Short Tandem

Variant alleles observed in short tandem repeat (STR) analysis of random Indian population

2019 ◽  
Vol 19 (1) ◽  
pp. 94
Author(s):  
Monika Chakravarty ◽  
Nidhi Singh ◽  
Damini Varshney ◽  
Dhruw Sharma ◽  
Naresh Kumar ◽  
...  
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Indian Population ◽  
Str Analysis ◽  
Variant Alleles ◽  
Short Tandem

Automated short tandem repeat (STR) analysis in forensic casework — a strategy for the future

1995 ◽  
Vol 16 (1) ◽  
pp. 1543-1552 ◽  
Author(s):  
Peter Gill ◽  
Colin P. Kimpton ◽  
Andrew Urquhart ◽  
Nicola Oldroyd ◽  
Emma S. Millican ◽  
...  
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Str Analysis ◽  
Forensic Casework ◽  
The Future ◽  
Short Tandem

scholarly journals Utilization of Short Tandem Repeat Analysis to Resolve Specimen Mislabeling in the Anatomic Pathology Laboratory

2021 ◽  
Vol 156 (Supplement_1) ◽  
pp. S112-S112
Author(s):  
T Tenney ◽  
B Robinson ◽  
H C Sullivan
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Single Patient ◽  
Pathology Laboratory ◽  
Str Analysis ◽  
Hematopoietic Stem ◽  
Anatomic Pathology ◽  
The Given ◽  
Short Tandem

Abstract Introduction/Objective A mislabeled specimen is an example of preanalytical error that can have significant consequences on patient care. These errors can be difficult to detect and resolve. One method to confirm genetic identity is short tandem repeat (STR) analysis, which is utilized in forensic investigations, paternity studies, and post- hematopoietic stem cell transplantation monitoring. Herein we present application of STR analysis to resolve a suspected specimen mislabeling prior to receipt in our anatomic pathology laboratory. Methods/Case Report DNA was extracted from paraffin embedded tissues. Chimerism testing was performed by STR analysis using the Globalfiler (ThermoFisher Scientific) and analyzed by Chimermarker (Softgenetics) automated chimerism software. Results (if a Case Study enter NA) Colon biopsies were received for a single patient (#1) with two requisition forms. Each specimen (A-F) was labeled with the patient’s name, with specimens A-D noted on first page of requisition and specimens E-F on the second requisition page. After the case was signed out, the lab was contacted looking for biopsy results on another patient (#2) who was seen on the same day as patient #1. Review of all the patients seen in the endoscopy suite on the given date raised suspicion that specimens E-F from the second page of the requisition actually pertained to patient #2. STR analysis performed on specimens confirmed that specimens E-F were genetically distinct from those labelled A-D. Tissue from a subsequent biopsy on patient #2 was analyzed by STR testing, which was identical to STR results performed on specimens E-F. Conclusion Here we utilized STR testing to resolve a suspected mislabeled specimen, allowing the appropriate diagnosis to be attributed to the correct patient. This is a unique application of a common method, which could be implemented in anatomic pathology laboratories to resolve cases of specimen mix-ups.

scholarly journals Individual Identification with Short Tandem Repeat Analysis and Collection of Secondary Information Using Microbiome Analysis

Genes ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 85
Author(s):  
Solip Lee ◽  
Heesang You ◽  
Songhee Lee ◽  
Yeongju Lee ◽  
Hee-Gyoo Kang ◽  
...  
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Individual Identification ◽  
Future Research ◽  
Str Analysis ◽  
Legal Evidence ◽  
Advantages And Disadvantages ◽  
Secondary Information ◽  
Generation Sequencing ◽  
Short Tandem

Forensic investigation is important to analyze evidence and facilitate the search for key individuals, such as suspects and victims in a criminal case. The forensic use of genomic DNA has increased with the development of DNA sequencing technology, thereby enabling additional analysis during criminal investigations when additional legal evidence is required. In this study, we used next-generation sequencing to facilitate the generation of complementary data in order to analyze human evidence obtained through short tandem repeat (STR) analysis. We examined the applicability and potential of analyzing microbial genome communities. Microbiological supplementation information was confirmed for two of four failed STR samples. Additionally, the accuracy of the gargle sample was confirmed to be as high as 100% and was highly likely to be classified as a body fluid sample. Our experimental method confirmed that anthropological and microbiological evidence can be obtained by performing two experiments with one extraction. We discuss the advantages and disadvantages of using these techniques, explore prospects in the forensic field, and highlight suggestions for future research.

scholarly journals Multiplex Sets for the Amplification of Polymorphic Short Tandem Repeat Loci — Silver Stain and Fluorescence Detection

BioTechniques ◽  
1996 ◽  
Vol 20 (5) ◽  
pp. 882-889 ◽  
Author(s):  
Ann M. Lins ◽  
Cynthia J. Sprecher ◽  
Christoph Puers ◽  
James W. Schumm
Keyword(s):  
Fluorescence Detection ◽  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Silver Stain ◽  
Short Tandem Repeat Loci ◽  
Short Tandem

Multiplexed short tandem repeat polymorphisms of the Weber 8A set of markers using tailed primers and infrared fluorescence detection

1998 ◽  
Vol 19 (18) ◽  
pp. 3079-3083 ◽  
Author(s):  
William S. Oetting ◽  
Catherine M. Armstrong ◽  
Shawn M. Ronan ◽  
Terri L. Young ◽  
Thomas A. Sellers ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Short Tandem
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