scholarly journals Visualization of microscale particle focusing in diluted and whole blood using particle trajectory analysis

Lab on a Chip ◽  
2012 ◽  
Vol 12 (12) ◽  
pp. 2199 ◽  
Author(s):  
Eugene J. Lim ◽  
Thomas J. Ober ◽  
Jon F. Edd ◽  
Gareth H. McKinley ◽  
Mehmet Toner
Keyword(s):  
Whole Blood ◽  
Trajectory Analysis ◽  
Particle Trajectory ◽  
Particle Focusing

Anomalous Diffusion: Single Particle Trajectory Analysis

2014 ◽  
Vol 59 (8) ◽  
pp. 775-780 ◽  
Author(s):  
A. Brodin ◽  
◽  
T. Turiv ◽  
V. Nazarenko ◽  
◽  
...  
Keyword(s):  
Anomalous Diffusion ◽  
Single Particle ◽  
Trajectory Analysis ◽  
Particle Trajectory

Nanostructured cobalt salts and their application in functional food products

2021 ◽  
pp. 495-502
Author(s):  
A.A. Krolevets ◽  
N.I. Myachikova ◽  
O.V. Binkovskaya ◽  
S.G. Glotova ◽  
E.M. Mamaeva ◽  
...  
Keyword(s):  
Functional Food ◽  
Trajectory Analysis ◽  
Particle Trajectory ◽  
Spherical Shape ◽  
Gellan Gum ◽  
Core Shell ◽  
Analysis Method ◽  
Cobalt Sulfate ◽  
Average Size ◽  
Polydispersity Coefficient

The paper provides data on the use of nanostructured cobalt sulfate in the production of marmalade as functional food for prophylactic purposes. The particle trajectory analysis method (NTA method) was used to determine the sizes of nanostructured cobalt sulfate. In this case, the smallest size (25.7 nm) is formed only in gellan gum with a “core : shell” ratio of 1:1. When the “core : shell” ratio in gellan gum is 1:3, the average size of nanocapsules is 49.9 nm. This result can be explained by the tighter packing of cobalt sulfate in the capsule. In sodium alginate, the average nanocapsule size is already 191 nm. The polydispersity coefficient in all studied shells and at different “core : shell” ratios are practically equal and amounts to 0.84–1.09, which corresponds to a spherical shape.

Fine Structure of Platelet Interaction with a New Microcrystalline Collagen Preparation

1974 ◽  
Vol 32 ◽  
pp. 58-59
Author(s):  
W. H. Zucker ◽  
R. G. Mason
Keyword(s):  
Fine Structure ◽  
Platelet Aggregation ◽  
Hydrochloric Acid ◽  
Whole Blood ◽  
Platelet Adhesion ◽  
Hemostatic Agent ◽  
Native Collagen ◽  
Fibrillar Morphology ◽  
Clinical Interest ◽  
New Form

Platelet adhesion initiates platelet aggregation and is an important component of the hemostatic process. Since the development of a new form of collagen as a topical hemostatic agent is of both basic and clinical interest, an ultrastructural and hematologic study of the interaction of platelets with the microcrystalline collagen preparation was undertaken.In this study, whole blood anticoagulated with EDTA was used in order to inhibit aggregation and permit study of platelet adhesion to collagen as an isolated event. The microcrystalline collagen was prepared from bovine dermal corium; milling was with sharp blades. The preparation consists of partial hydrochloric acid amine collagen salts and retains much of the fibrillar morphology of native collagen.

3-D organization of fibrinogen receptors using computer reconstruction and whole-mount microscopy

1988 ◽  
Vol 46 ◽  
pp. 30-31
Author(s):  
E. T. O'Toole ◽  
R. R. Hantgan ◽  
J. C. Lewis
Keyword(s):  
Whole Blood ◽  
Colloidal Gold ◽  
Blood Platelets ◽  
Cell Contact ◽  
Computer Assisted ◽  
Adherent Cells ◽  
Differential Centrifugation ◽  
Computer Reconstruction ◽  
Sds Page ◽  
Whole Mount

Thrombocytes (TC), the avian equivalent of blood platelets, support hemostasis by aggregating at sites of injury. Studies in our lab suggested that fibrinogen (fib) is a requisite cofactor for TC aggregation but operates by an undefined mechanism. To study the interaction of fib with TC and to identify fib receptors on cells, fib was purified from pigeon plasma, conjugated to colloidal gold and used both to facilitate aggregation and as a receptor probe. Described is the application of computer assisted reconstruction and stereo whole mount microscopy to visualize the 3-D organization of fib receptors at sites of cell contact in TC aggregates and on adherent cells.Pigeon TC were obtained from citrated whole blood by differential centrifugation, washed with Ca++ free Hank's balanced salts containing 0.3% EDTA (pH 6.5) and resuspended in Ca++ free Hank's. Pigeon fib was isolated by precipitation with PEG-1000 and the purity assessed by SDS-PAGE. Fib was conjugated to 25nm colloidal gold by vortexing and the conjugates used as the ligand to identify fib receptors.

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