Application of click chemistry to the production of DNA microarrays

Lab on a Chip ◽  
2012 ◽  
Vol 12 (6) ◽  
pp. 1151 ◽  
Author(s):  
Barbara Uszczyńska ◽  
Tomasz Ratajczak ◽  
Emilia Frydrych ◽  
Hieronim Maciejewski ◽  
Marek Figlerowicz ◽  
...  
Keyword(s):  
Click Chemistry ◽  
Dna Microarrays

Direct Covalent Attachment of DNA Microarrays by Rapid Thiol–Ene “Click” Chemistry

2014 ◽  
Vol 25 (3) ◽  
pp. 618-627 ◽  
Author(s):  
Jorge Escorihuela ◽  
María-José Bañuls ◽  
Santiago Grijalvo ◽  
Ramón Eritja ◽  
Rosa Puchades ◽  
...  
Keyword(s):  
Click Chemistry ◽  
Dna Microarrays ◽  
Covalent Attachment

Anti-Diabetic Activity of a Mineraloid Isolate, in vitro and in Genetically Diabetic Mice

2011 ◽  
Vol 81 (1) ◽  
pp. 34-42 ◽  
Author(s):  
Joel Deneau ◽  
Taufeeq Ahmed ◽  
Roger Blotsky ◽  
Krzysztof Bojanowski
Keyword(s):  
Dna Microarrays ◽  
Human Fibroblasts ◽  
Diabetic Animal ◽  
In Vitro Tests ◽  
Diabetic Mice ◽  
Fossil Material ◽  
Expression Of Genes ◽  
Enhanced Expression ◽  
Genetically Diabetic Mice

Type II diabetes is a metabolic disease mediated through multiple molecular pathways. Here, we report anti-diabetic effect of a standardized isolate from a fossil material - a mineraloid leonardite - in in vitro tests and in genetically diabetic mice. The mineraloid isolate stimulated mitochondrial metabolism in human fibroblasts and this stimulation correlated with enhanced expression of genes coding for mitochondrial proteins such as ATP synthases and ribosomal protein precursors, as measured by DNA microarrays. In the diabetic animal model, consumption of the Totala isolate resulted in decreased weight gain, blood glucose, and glycated hemoglobin. To our best knowledge, this is the first description ever of a fossil material having anti-diabetic activity in pre-clinical models.

Can DNA microarrays change diagnostics and therapy of lung cancer?

2006 ◽  
Vol 54 (S 1) ◽  
Author(s):  
HS Hofmann ◽  
A Simm ◽  
G Hansen ◽  
RJ Scheubel ◽  
RE Silber ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Dna Microarrays ◽  
Diagnostics And Therapy

Click Chemistry Expedited Radiosynthesis: Sulfur [18F]fluoride Exchange of Aryl Fluorosulfates

2019 ◽  
Author(s):  
Qinheng Zheng ◽  
Hongtao Xu ◽  
Hua Wang ◽  
Wen-Ge Han Du ◽  
Nan Wang ◽  
...  
Keyword(s):  
Click Chemistry ◽  
High Performance ◽  
Isotopic Exchange ◽  
Tumor Imaging ◽  
Radiochemical Yield ◽  
Exchange Method ◽  
Molar Activity ◽  
Positron Emission ◽  
Sulfur Fluoride

The lack of simple, efficient [<sup>18</sup>F]fluorination processes and new target-specific organofluorine probes remains the major challenge of fluorine-18-based positron emission tomography (PET). We report here a fast isotopic exchange method for the radiosynthesis of aryl [<sup>18</sup>F]fluorosulfate based PET agents enabled by the emerging sulfur fluoride exchange (SuFEx) click chemistry. The method has been applied to the fully-automated <sup>18</sup>F-radiolabeling of twenty-five structurally diverse aryl fluorosulfates with excellent radiochemical yield (83–100%) and high molar activity (up to 281 GBq µmol<sup>–1</sup>) at room temperature in 30 seconds. The purification of radiotracers requires no time-consuming high-performance liquid chromatography (HPLC), but rather a simple cartridge filtration. The utility of aryl [<sup>18</sup>F]fluorosulfate is demonstrated by the <i>in vivo</i> tumor imaging by targeting poly(ADP-ribose) polymerase 1 (PARP1).

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