Direct Covalent Attachment of DNA Microarrays by Rapid Thiol–Ene “Click” Chemistry

2014 ◽  
Vol 25 (3) ◽  
pp. 618-627 ◽  
Author(s):  
Jorge Escorihuela ◽  
María-José Bañuls ◽  
Santiago Grijalvo ◽  
Ramón Eritja ◽  
Rosa Puchades ◽  
...  
Keyword(s):  
Click Chemistry ◽  
Dna Microarrays ◽  
Covalent Attachment

Comparison between Different Strategies of Covalent Attachment of DNA to Glass Surfaces to Build DNA Microarrays

2000 ◽  
Vol 280 (1) ◽  
pp. 143-150 ◽  
Author(s):  
Nathalie Zammatteo ◽  
Laurent Jeanmart ◽  
Sandrine Hamels ◽  
Stéphane Courtois ◽  
Pierre Louette ◽  
...  
Keyword(s):  
Dna Microarrays ◽  
Covalent Attachment ◽  
Glass Surfaces

Convenient Preparation of Bactericidal Hydrogels by Covalent Attachment of Stabilized Antimicrobial Peptides Using Thiol–ene Click Chemistry

2014 ◽  
Vol 3 (5) ◽  
pp. 477-480 ◽  
Author(s):  
Rik T. C. Cleophas ◽  
Martijn Riool ◽  
H. (Linda) C. Quarles van Ufford ◽  
Sebastian A. J. Zaat ◽  
John A. W. Kruijtzer ◽  
...  
Keyword(s):  
Antimicrobial Peptides ◽  
Click Chemistry ◽  
Covalent Attachment ◽  
Convenient Preparation

scholarly journals Covalent attachment of chitosan to graphene via click chemistry for superior antibacterial activity

2020 ◽  
Vol 1 (4) ◽  
pp. 579-583 ◽  
Author(s):  
Jing Huang ◽  
Zhenyao Yin ◽  
Jinggao Wu
Keyword(s):  
Antibacterial Activity ◽  
Click Chemistry ◽  
Covalent Attachment ◽  
Diazonium Salts ◽  
Graphene Sheets ◽  
Chemically Converted Graphene

Chemically converted graphene sheets are functionalized by treatment with aryl diazonium salts via click chemistry.

Application of click chemistry to the production of DNA microarrays

Lab on a Chip ◽  
2012 ◽  
Vol 12 (6) ◽  
pp. 1151 ◽  
Author(s):  
Barbara Uszczyńska ◽  
Tomasz Ratajczak ◽  
Emilia Frydrych ◽  
Hieronim Maciejewski ◽  
Marek Figlerowicz ◽  
...  
Keyword(s):  
Click Chemistry ◽  
Dna Microarrays

scholarly journals Vapor Sublimation and Deposition to Fabricate a Porous Methyl Propiolate-Functionalized Poly-p-xylylene Material for Copper-Free Click Chemistry

Polymers ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 786
Author(s):  
Chin-Yun Lee ◽  
Shu-Man Hu ◽  
Jia-Qi Xiao ◽  
Yu-Ming Chang ◽  
Tatsuya Kusanagi ◽  
...  
Keyword(s):  
Porous Materials ◽  
Click Chemistry ◽  
Vapor Deposition ◽  
Deposition Process ◽  
Covalent Attachment ◽  
Polymerization Process ◽  
Synthetic Approach ◽  
Porous Structures ◽  
Methyl Propiolate ◽  
Porous Poly

Conventional porous materials are mostly synthesized in solution-based methods involving solvents and initiators, and the functionalization of these porous materials usually requires additional and complex steps. In the current study, a methyl propiolate-functionalized porous poly-p-xylylene material was fabricated based on a unique vapor sublimation and deposition process. The process used a water solution and ice as the template with a customizable shape and dimensions, and the conventional chemical vapor deposition (CVD) polymerization of poly-p-xylylene on such an ice template formed a three-dimensional, porous poly-p-xylylene material with interconnected porous structures. More importantly, the functionality of methyl propiolate was well preserved by using methyl propiolate-substituted [2,2]-paracyclophane during the vapor deposition polymerization process and was installed in one step on the final porous poly-p-xylylene products. This functionality exhibited an intact structure and reactivity during the proposed vapor sublimation and deposition process and was proven to have no decomposition or side products after further characterization and conjugation experiments. The electron-withdrawing methyl propiolate group readily provided efficient alkynes as click azide-terminated molecules under copper-free and mild conditions at room temperature and in environmentally friendly solvents, such as water. The resulting methyl propiolate-functionalized porous poly-p-xylylene exhibited interface properties with clickable specific covalent attachment toward azide-terminated target molecules, which are widely available for drugs and biomolecules. The fabricated functional porous materials represent an advanced material featuring porous structures, a straightforward synthetic approach, and precise and controlled interface click chemistry, rendering long-term stability and efficacy to conjugate target functionalities that are expected to attract a variety of new applications.

Anti-Diabetic Activity of a Mineraloid Isolate, in vitro and in Genetically Diabetic Mice

2011 ◽  
Vol 81 (1) ◽  
pp. 34-42 ◽  
Author(s):  
Joel Deneau ◽  
Taufeeq Ahmed ◽  
Roger Blotsky ◽  
Krzysztof Bojanowski
Keyword(s):  
Dna Microarrays ◽  
Human Fibroblasts ◽  
Diabetic Animal ◽  
In Vitro Tests ◽  
Diabetic Mice ◽  
Fossil Material ◽  
Expression Of Genes ◽  
Enhanced Expression ◽  
Genetically Diabetic Mice

Type II diabetes is a metabolic disease mediated through multiple molecular pathways. Here, we report anti-diabetic effect of a standardized isolate from a fossil material - a mineraloid leonardite - in in vitro tests and in genetically diabetic mice. The mineraloid isolate stimulated mitochondrial metabolism in human fibroblasts and this stimulation correlated with enhanced expression of genes coding for mitochondrial proteins such as ATP synthases and ribosomal protein precursors, as measured by DNA microarrays. In the diabetic animal model, consumption of the Totala isolate resulted in decreased weight gain, blood glucose, and glycated hemoglobin. To our best knowledge, this is the first description ever of a fossil material having anti-diabetic activity in pre-clinical models.

Can DNA microarrays change diagnostics and therapy of lung cancer?

2006 ◽  
Vol 54 (S 1) ◽  
Author(s):  
HS Hofmann ◽  
A Simm ◽  
G Hansen ◽  
RJ Scheubel ◽  
RE Silber ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Dna Microarrays ◽  
Diagnostics And Therapy
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