scholarly journals In situ electroporation of surface-bound siRNAs in microwell arrays

Lab on a Chip ◽  
2012 ◽  
Vol 12 (5) ◽  
pp. 939 ◽  
Author(s):  
Tilak Jain ◽  
Adrian Papas ◽  
Amol Jadhav ◽  
Ryan McBride ◽  
Enrique Saez
Keyword(s):  
In Situ Electroporation

scholarly journals Recovery of Adherent Cells after In Situ Electroporation Monitored Electrically

BioTechniques ◽  
2002 ◽  
Vol 33 (2) ◽  
pp. 348-357 ◽  
Author(s):  
Joachim Wegener ◽  
Charles R. Keese ◽  
Ivar Giaever
Keyword(s):  
Adherent Cells ◽  
In Situ Electroporation

Impedance analysis of adherent cells after in situ electroporation: Non-invasive monitoring during intracellular manipulations

2011 ◽  
Vol 26 (12) ◽  
pp. 4720-4727 ◽  
Author(s):  
Judith A. Stolwijk ◽  
Christoph Hartmann ◽  
Poonam Balani ◽  
Silke Albermann ◽  
Charles R. Keese ◽  
...  
Keyword(s):  
Impedance Analysis ◽  
Adherent Cells ◽  
Invasive Monitoring ◽  
Non Invasive ◽  
In Situ Electroporation

Design and Implementation of a Microelectrode Assembly for Use on Noncontact In Situ Electroporation of Adherent Cells

2012 ◽  
Vol 245 (10) ◽  
pp. 617-624 ◽  
Author(s):  
Tomás García-Sánchez ◽  
Beatriz Sánchez-Ortiz ◽  
Ingrid Vila ◽  
Maria Guitart ◽  
Javier Rosell ◽  
...  
Keyword(s):  
Adherent Cells ◽  
Design And Implementation ◽  
In Situ Electroporation

In situ electroporation of large numbers of cells using minimal volumes of material

2003 ◽  
Vol 317 (1) ◽  
pp. 124-128 ◽  
Author(s):  
Leda Raptis ◽  
Valerie Balboa ◽  
Tina Hsu ◽  
Adina Vultur ◽  
James Turkson ◽  
...  
Keyword(s):  
Large Numbers ◽  
In Situ Electroporation

scholarly journals Impedance analysis of adherent cells after in situ electroporation-mediated delivery of bioactive proteins, DNA and nanoparticles in µL-volumes

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Judith A. Stolwijk ◽  
Joachim Wegener
Keyword(s):  
Cell Response ◽  
Cell Biology ◽  
Impedance Analysis ◽  
Rnase A ◽  
Adherent Cells ◽  
Animal Cells ◽  
Impedance Sensing ◽  
Time Resolved ◽  
In Situ Electroporation

AbstractSpecific intracellular manipulation of animal cells is a persistent goal in experimental cell biology. Such manipulations allow precise and targeted interference with signaling cascades, metabolic pathways, or bi-molecular interactions for subsequent tracking of functional consequences. However, most biomolecules capable of molecular recognition are membrane impermeable. The ability to introduce these molecules into the cytoplasm and then to apply appropriate readouts to monitor the corresponding cell response could prove to be an important research tool. This study describes such an experimental approach combining in situ electroporation (ISE) as a means to efficiently deliver biomolecules to the cytoplasm with an impedance-based, time-resolved analysis of cell status using electric cell-substrate impedance sensing (ECIS). In this approach, gold-film electrodes, deposited on the bottom of regular culture dishes, are used for both electroporation and monitoring. The design of the electrode layout and measurement chamber allows working with sample volumes as small as 10 µL. A miniaturized setup for combined electroporation and impedance sensing (µISE-ECIS) was applied to load different adherent cells with bioactive macromolecules including enzymes, antibodies, nucleic acids and quantum dot nanoparticles. The cell response after loading the cytoplasm with RNase A or cytochrome c (in the presence or absence of caspase inhibitors) was tracked by non-invasive impedance readings in real-time.

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