Impedance analysis of adherent cells after in situ electroporation: Non-invasive monitoring during intracellular manipulations

2011 ◽  
Vol 26 (12) ◽  
pp. 4720-4727 ◽  
Author(s):  
Judith A. Stolwijk ◽  
Christoph Hartmann ◽  
Poonam Balani ◽  
Silke Albermann ◽  
Charles R. Keese ◽  
...  
Keyword(s):  
Impedance Analysis ◽  
Adherent Cells ◽  
Invasive Monitoring ◽  
Non Invasive ◽  
In Situ Electroporation

scholarly journals Impedance analysis of adherent cells after in situ electroporation-mediated delivery of bioactive proteins, DNA and nanoparticles in µL-volumes

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Judith A. Stolwijk ◽  
Joachim Wegener
Keyword(s):  
Cell Response ◽  
Cell Biology ◽  
Impedance Analysis ◽  
Rnase A ◽  
Adherent Cells ◽  
Animal Cells ◽  
Impedance Sensing ◽  
Time Resolved ◽  
In Situ Electroporation

AbstractSpecific intracellular manipulation of animal cells is a persistent goal in experimental cell biology. Such manipulations allow precise and targeted interference with signaling cascades, metabolic pathways, or bi-molecular interactions for subsequent tracking of functional consequences. However, most biomolecules capable of molecular recognition are membrane impermeable. The ability to introduce these molecules into the cytoplasm and then to apply appropriate readouts to monitor the corresponding cell response could prove to be an important research tool. This study describes such an experimental approach combining in situ electroporation (ISE) as a means to efficiently deliver biomolecules to the cytoplasm with an impedance-based, time-resolved analysis of cell status using electric cell-substrate impedance sensing (ECIS). In this approach, gold-film electrodes, deposited on the bottom of regular culture dishes, are used for both electroporation and monitoring. The design of the electrode layout and measurement chamber allows working with sample volumes as small as 10 µL. A miniaturized setup for combined electroporation and impedance sensing (µISE-ECIS) was applied to load different adherent cells with bioactive macromolecules including enzymes, antibodies, nucleic acids and quantum dot nanoparticles. The cell response after loading the cytoplasm with RNase A or cytochrome c (in the presence or absence of caspase inhibitors) was tracked by non-invasive impedance readings in real-time.

scholarly journals Recovery of Adherent Cells after In Situ Electroporation Monitored Electrically

BioTechniques ◽  
2002 ◽  
Vol 33 (2) ◽  
pp. 348-357 ◽  
Author(s):  
Joachim Wegener ◽  
Charles R. Keese ◽  
Ivar Giaever
Keyword(s):  
Adherent Cells ◽  
In Situ Electroporation

scholarly journals Ultrasound imaging of apoptosis: high-resolution non-invasive monitoring of programmed cell death in vitro, in situ and in vivo

1999 ◽  
Vol 81 (3) ◽  
pp. 520-527 ◽  
Author(s):  
G J Czarnota ◽  
M C Kolios ◽  
J Abraham ◽  
M Portnoy ◽  
F P Ottensmeyer ◽  
...  
Keyword(s):  
Cell Death ◽  
High Resolution ◽  
Programmed Cell Death ◽  
Ultrasound Imaging ◽  
Invasive Monitoring ◽  
Non Invasive

scholarly journals Complementary use of Optical Coherence Tomography (OCT) and Reflection FTIR spectroscopy for in-situ non-invasive monitoring of varnish removal from easel paintings

2018 ◽  
Vol 138 ◽  
pp. 7-18 ◽  
Author(s):  
Magdalena Iwanicka ◽  
Patrizia Moretti ◽  
Saskia van Oudheusden ◽  
Marcin Sylwestrzak ◽  
Laura Cartechini ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Ftir Spectroscopy ◽  
Invasive Monitoring ◽  
Optical Coherence ◽  
Non Invasive

Design and Implementation of a Microelectrode Assembly for Use on Noncontact In Situ Electroporation of Adherent Cells

2012 ◽  
Vol 245 (10) ◽  
pp. 617-624 ◽  
Author(s):  
Tomás García-Sánchez ◽  
Beatriz Sánchez-Ortiz ◽  
Ingrid Vila ◽  
Maria Guitart ◽  
Javier Rosell ◽  
...  
Keyword(s):  
Adherent Cells ◽  
Design And Implementation ◽  
In Situ Electroporation

Efficiency of diagnosis and treatment non-invasive and microinvasive sgamous cell cervical cancer

2020 ◽  
pp. 71-74
Author(s):  
M.M. Melnyk ◽  
◽  
S.V. Nespradko ◽  
I.V. Goncharuk ◽  
M.V. Marchenko ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Key Words ◽  
National Cancer Institute ◽  
Reproductive Function ◽  
Diagnosis And Treatment ◽  
Non Invasive ◽  
Corpus Uteri ◽  
Complex Program ◽  
Early Cervical Cancer

The objective: analyse the effectiveness of diagnosis and treatment for early cervical cancer. Materials and methods. Analysed 107 cases of women’s disease on CIN ІІІ, cancer in situ, they were on treatment in National cancer institute and Kyiv dictrict cancer dispensary from 2010 till 2015 years. Results. Diagnosed percent relapse CIN ІІІ, cancer in situ contain 4.57% uninvasive and invasive form – 0.94%. Conclusion. According diagnostic CIN ІІ and CIN ІІІ is recommended to do treatment conization and dynamic dispensary observation. Are making complex program of infection HPV16, 18. In appering of margins resection some elements of tumor after wider conization by forms of cancer in situ. Many of expansive burns in cervical glands, in making of reproductive function, going disease (nodel leiomyoma of corpus uteri etc). In perspective is accept the notion of looking after and screening research of considering infection HPV16, 18 on CIN І, CIN ІІ. Key words: cervical cancer, сancer in situ, CIN І–ІІІ, diagnostic, treatment, conization.

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