Biocompatible magnetic cellulose–chitosan hybrid gel microspheres reconstituted from ionic liquids for enzyme immobilization

2012 ◽  
Vol 22 (30) ◽  
pp. 15085 ◽  
Author(s):  
Zhen Liu ◽  
Haisong Wang ◽  
Bin Li ◽  
Chao Liu ◽  
Yijun Jiang ◽  
...  
Keyword(s):  
Ionic Liquids ◽  
Enzyme Immobilization ◽  
Hybrid Gel

Synthesis of polysaccharide hybrid gel in ionic liquids via radiation-induced crosslinking

2019 ◽  
Vol 159 ◽  
pp. 133-138 ◽  
Author(s):  
Atsushi Kimura ◽  
Naotsugu Nagasawa ◽  
Mitsumasa Taguchi
Keyword(s):  
Ionic Liquids ◽  
Hybrid Gel ◽  
Radiation Induced

scholarly journals Enzyme Immobilization in Polymerized Ionic Liquids-based Hydrogels for Active and Reusable Biocatalysts

SynOpen ◽  
2018 ◽  
Vol 02 (02) ◽  
pp. 0192-0199 ◽  
Author(s):  
A. Grollmisch ◽  
U. Kragl ◽  
J. Großeheilmann
Keyword(s):  
Ionic Liquids ◽  
Enzyme Immobilization ◽  
Kinetic Resolution ◽  
Enantiomeric Excess ◽  
Catalytic Activities ◽  
Nonpolar Solvents ◽  
Novozyme 435 ◽  
Versatile Tool ◽  
Full Conversion ◽  
Immobilization Method

Enzyme immobilization in polymerized ionic liquids (PILs) promises to be a versatile tool for simple recovery and reuse of catalysts. In this study, a raw extract of CalB was encapsulated in poly(VEImBr) and assessed with respect to solvent, temperature, amount of enzyme, leaching behavior, and reusability on the example of the kinetic resolution of rac-1-phenylethanol with vinyl acetate. This immobilization method increased the enzyme activity of the CalB raw extract in comparison to the non-immobilized enzyme. The desired product was synthesized with high enantiomeric excess (ee) and no leaching of active enzyme was observed in the experiments. The immobilization method was compared to Novozyme 435 and Lipozyme RM IM, as commercially available immobilisates. Nonpolar solvents, including n-heptane and n-dodecane, proved to be the best reaction solvents, showing nearly full conversion and high catalytic activities. The encapsulated ­lipase was easily recovered from the reaction mixture and reused for ten cycles.

Environmentally Friendly Preparation of Magnetic Composites Featuring Proteolytic Properties

INEOS OPEN ◽  
2020 ◽  
Author(s):  
N. A. Samoilova ◽  
Keyword(s):  
Enzyme Immobilization ◽  
Maleic Acid ◽  
Environmentally Friendly ◽  
Synthetic Polymer ◽  
Magnetic Composites ◽  
Interpolyelectrolyte Complex ◽  
Hydrolysis Rates ◽  
Poly Ethylene ◽  
Noncovalent Binding

The enzyme-containing magnetic composites are presented. The magnetic matrix for enzyme immobilization is obtained by sequential application of an amine-containing polysaccharide—chitosan and a synthetic polymer—poly(ethylene-alt-maleic acid) to the magnetite microparticles to form the interpolyelectrolyte complex shell. Then, the enzyme (trypsin) is immobilized by covalent or noncovalent binding. Thus, the suggested composites can be readily obtained in the environmentally friendly manner. The enzyme capacity of the resulting composites reaches 28.0–32.6 mg/g. The maximum hydrolysis rates of the H-Val-Leu-Lys-pNA substrate provided by these composites range within 0.60·10–7–0.77·10–7 M/min.

Investigation of Various Ionic Liquids and Catalyst Materials for Lithium-Oxygen Batteries

2011 ◽  
pp. 110923034559006
Author(s):  
Arnd Garsuch ◽  
D. Michael Badine ◽  
Klaus Leitner ◽  
Luiz H. S. Gasparotto ◽  
Natalia Borisenko ◽  
...  
Keyword(s):  
Ionic Liquids ◽  
Lithium Oxygen Batteries
Sign in / Sign up

Export Citation Format

Share Document