Boronic acid modified magnetic nanoparticles for enrichment of glycoproteins via azide and alkyne click chemistry

2012 ◽  
Vol 22 (32) ◽  
pp. 16520 ◽  
Author(s):  
Xihao Zhang ◽  
Xiwen He ◽  
Langxing Chen ◽  
Yukui Zhang
Keyword(s):  
Magnetic Nanoparticles ◽  
Click Chemistry ◽  
Boronic Acid ◽  
Modified Magnetic Nanoparticles

Boronic acid-functionalized iron oxide magnetic nanoparticles via distillation–precipitation polymerization and thiol–yne click chemistry for the enrichment of glycoproteins

2018 ◽  
Vol 42 (21) ◽  
pp. 17331-17338 ◽  
Author(s):  
Changfen Bi ◽  
Sitong Zhang ◽  
Yiliang Li ◽  
Xiwen He ◽  
Langxing Chen ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Magnetic Nanoparticles ◽  
Click Chemistry ◽  
Iron Oxide Nanoparticles ◽  
Boronic Acid ◽  
Phenylboronic Acid ◽  
Precipitation Polymerization ◽  
Oxide Nanoparticles ◽  
Distillation Precipitation Polymerization ◽  
Iron Oxide Magnetic Nanoparticles

Synthesis of phenylboronic acid functionalized iron oxide nanoparticles for glycoprotein enrichment via distillation–precipitation polymerization combined with thiol–yne click chemistry.

scholarly journals Polymer Brush-Modified Magnetic Nanoparticles for His-Tagged Protein Purification

Langmuir ◽  
2011 ◽  
Vol 27 (6) ◽  
pp. 3106-3112 ◽  
Author(s):  
Fei Xu ◽  
James H. Geiger ◽  
Gregory L. Baker ◽  
Merlin L. Bruening
Keyword(s):  
Magnetic Nanoparticles ◽  
Protein Purification ◽  
Polymer Brush ◽  
Modified Magnetic Nanoparticles

scholarly journals Selective Capture and Identification of Methicillin-Resistant Staphylococcus aureus by Combining Aptamer-Modified Magnetic Nanoparticles and Mass Spectrometry

2021 ◽  
Vol 22 (12) ◽  
pp. 6571
Author(s):  
Yu-Chen Liu ◽  
Katragunta Kumar ◽  
Cheng-Hsiu Wu ◽  
Kai-Chih Chang ◽  
Cheng-Kang Chiang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Staphylococcus Aureus ◽  
Magnetic Nanoparticles ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Capture Rate ◽  
Mass Spectrometry Analysis ◽  
Ionization Mass ◽  
Methicillin Resistant ◽  
Spectrometry Analysis ◽  
Modified Magnetic Nanoparticles

A nucleic acid aptamer that specifically recognizes methicillin-resistant Staphylococcus aureus (MRSA) has been immobilized on magnetic nanoparticles to capture the target bacteria prior to mass spectrometry analysis. After the MRSA species were captured, they were further eluted from the nanoparticles and identified using matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS). The combination of aptamer-based capture/enrichment and MS analysis of microorganisms took advantage of the selectivity of both techniques and should enhance the accuracy of MRSA identification. The capture and elution efficiencies for MRSA were optimized by examining factors such as incubation time, temperature, and elution solvents. The aptamer-modified magnetic nanoparticles showed a capture rate of more than 90% under the optimized condition, whereas the capture rates were less than 11% for non-target bacteria. The as-prepared nanoparticles exhibited only a 5% decrease in the capture rate and a 9% decrease in the elution rate after 10 successive cycles of utilization. Most importantly, the aptamer-modified nanoparticles revealed an excellent selectivity towards MRSA in bacterial mixtures. The capture of MRSA at a concentration of 102 CFU/mL remained at a good percentage of 82% even when the other two species were at 104 times higher concentration (106 CFU/mL). Further, the eluted MRSA bacteria were successfully identified using MALDI mass spectrometry.

Sign in / Sign up

Export Citation Format

Share Document