A novel conjugate of a cell-penetrating peptide and a ferrocenyl amino acid: a potential electrochemical sensor for living cells?

2012 ◽  
Vol 41 (21) ◽  
pp. 6396 ◽  
Author(s):  
Jan Hoyer ◽  
Andrea Hunold ◽  
Hans-Günther Schmalz ◽  
Ines Neundorf
Keyword(s):  
Amino Acid ◽  
Electrochemical Sensor ◽  
Living Cells ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating ◽  
A Cell

scholarly journals An ω-3, but Not an ω-6 Polyunsaturated Fatty Acid Decreases Membrane Dipole Potential and Stimulates Endo-Lysosomal Escape of Penetratin

2021 ◽  
Vol 9 ◽  
Author(s):  
Florina Zakany ◽  
Mate Szabo ◽  
Gyula Batta ◽  
Levente Kárpáti ◽  
István M. Mándity ◽  
...  
Keyword(s):  
Linolenic Acid ◽  
Measurement Techniques ◽  
Transmembrane Proteins ◽  
Living Cells ◽  
Cell Penetrating Peptide ◽  
Dipole Potential ◽  
Membrane Dipole Potential ◽  
Cell Penetrating ◽  
A Cell ◽  
Novel Method

Although the largely positive intramembrane dipole potential (DP) may substantially influence the function of transmembrane proteins, its investigation is deeply hampered by the lack of measurement techniques suitable for high-throughput examination of living cells. Here, we describe a novel emission ratiometric flow cytometry method based on F66, a 3-hydroxiflavon derivative, and demonstrate that 6-ketocholestanol, cholesterol and 7-dehydrocholesterol, saturated stearic acid (SA) and ω-6 γ-linolenic acid (GLA) increase, while ω-3 α-linolenic acid (ALA) decreases the DP. These changes do not correlate with alterations in cell viability or membrane fluidity. Pretreatment with ALA counteracts, while SA or GLA enhances cholesterol-induced DP elevations. Furthermore, ALA (but not SA or GLA) increases endo-lysosomal escape of penetratin, a cell-penetrating peptide. In summary, we have developed a novel method to measure DP in large quantities of individual living cells and propose ALA as a physiological DP lowering agent facilitating cytoplasmic entry of penetratin.

Protein Profiling of Active Cysteine Cathepsins in Living Cells Using an Activity-Based Probe Containing a Cell-Penetrating Peptide

2012 ◽  
Vol 11 (12) ◽  
pp. 5763-5772 ◽  
Author(s):  
Fengkai Fan ◽  
Si Nie ◽  
Eric B. Dammer ◽  
Duc M. Duong ◽  
Deng Pan ◽  
...  
Keyword(s):  
Living Cells ◽  
Protein Profiling ◽  
Cell Penetrating Peptide ◽  
Cysteine Cathepsins ◽  
Cell Penetrating ◽  
A Cell

scholarly journals A Cell-Penetrating Peptide with a Guanidinylethyl Amine Structure Directed to Gene Delivery

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Makoto Oba ◽  
Takuma Kato ◽  
Kaori Furukawa ◽  
Masakazu Tanaka
Keyword(s):  
Gene Delivery ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating ◽  
Amine Structure ◽  
A Cell

Pharmacokinetics, Biodistribution, Stability and Toxicity of a Cell-Penetrating Peptide−Morpholino Oligomer Conjugate

2007 ◽  
Vol 18 (4) ◽  
pp. 1325-1331 ◽  
Author(s):  
Adams Amantana ◽  
Hong M. Moulton ◽  
Melissa L. Cate ◽  
Muralimohan T. Reddy ◽  
Tom Whitehead ◽  
...  
Keyword(s):  
Cell Penetrating Peptide ◽  
Cell Penetrating ◽  
A Cell

Matrix metalloproteinase-1 induces cleavage of exogenous alphab-crystallin transduced by a cell-penetrating peptide

2011 ◽  
Vol 112 (9) ◽  
pp. 2454-2462
Author(s):  
Seung Won Yang ◽  
Seung-Min Lee ◽  
Eun Young Choi ◽  
Kyung Hye Lee ◽  
Soo Hyuk Kim ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Cell Penetrating Peptide ◽  
Matrix Metalloproteinase 1 ◽  
Cell Penetrating ◽  
A Cell

scholarly journals Tumor targeting of a cell penetrating peptide by fusing with a pH-sensitive histidine-glutamate co-oligopeptide

Biomaterials ◽  
2014 ◽  
Vol 35 (13) ◽  
pp. 4082-4087 ◽  
Author(s):  
Likun Fei ◽  
Li-Peng Yap ◽  
Peter S. Conti ◽  
Wei-Chiang Shen ◽  
Jennica L. Zaro
Keyword(s):  
Tumor Targeting ◽  
Ph Sensitive ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating ◽  
A Cell

scholarly journals Molecular Targeting of Cancer-Associated PCNA Interactions in Pancreatic Ductal Adenocarcinoma Using a Cell-Penetrating Peptide

2020 ◽  
Vol 17 ◽  
pp. 250-256
Author(s):  
Shanna J. Smith ◽  
Caroline M. Li ◽  
Robert G. Lingeman ◽  
Robert J. Hickey ◽  
Yilun Liu ◽  
...  
Keyword(s):  
Pancreatic Ductal Adenocarcinoma ◽  
Ductal Adenocarcinoma ◽  
Molecular Targeting ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating ◽  
A Cell
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