Novel far-visible and near-infrared pH probes based on styrylcyanine for imaging intracellular pH in live cells

2012 ◽  
Vol 48 (91) ◽  
pp. 11202 ◽  
Author(s):  
Li Fan ◽  
Yue-Jun Fu ◽  
Qiao-Ling Liu ◽  
Dong-Tao Lu ◽  
Chuan Dong ◽  
...  
Keyword(s):  
Intracellular Ph ◽  
Near Infrared ◽  
Live Cells ◽  
Ph Probes

scholarly journals A Near-Infrared Fluorescent Probe Based on a FRET Rhodamine Donor Linked to a Cyanine Acceptor for Sensitive Detection of Intracellular pH Alternations

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2679 ◽  
Author(s):  
Yibin Zhang ◽  
Jianheng Bi ◽  
Shuai Xia ◽  
Wafa Mazi ◽  
Shulin Wan ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Intracellular Ph ◽  
Near Infrared ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Sensitive Detection ◽  
Solution Ph ◽  
Acidic Conditions ◽  
Ph Level ◽  
Rhodamine Dye

A fluorescence resonance energy transfer (FRET)-based near-infrared fluorescent probe (B+) for double-checked sensitive detection of intracellular pH changes has been synthesized by binding a near-infrared rhodamine donor to a near-infrared cyanine acceptor through robust C-N bonds via a nucleophilic substitution reaction. To demonstrate the double-checked advantages of probe B+, a near-infrared probe (A) was also prepared by modification of a near-infrared rhodamine dye with ethylenediamine to produce a closed spirolactam residue. Under basic conditions, probe B+ shows only weak fluorescence from the cyanine acceptor while probe A displays nonfluorescence due to retention of the closed spirolactam form of the rhodamine moiety. Upon decrease in solution pH level, probe B+ exhibits a gradual fluorescence increase from rhodamine and cyanine constituents at 623 nm and 743 nm respectively, whereas probe A displays fluorescence increase at 623 nm on the rhodamine moiety as acidic conditions leads to the rupture of the probe spirolactam rings. Probes A and B+ have successfully been used to monitor intracellular pH alternations and possess pKa values of 5.15 and 7.80, respectively.

Reversible Self-Assembly of Nanoprobes in Live Cells for Dynamic Intracellular pH Imaging

ACS Nano ◽  
2019 ◽  
Author(s):  
Bo Dong ◽  
Shuangli Du ◽  
Chunxiao Wang ◽  
Haohao Fu ◽  
Qiang Li ◽  
...  
Keyword(s):  
Intracellular Ph ◽  
Self Assembly ◽  
Live Cells ◽  
Ph Imaging

Near-Infrared-Fluorescence-Enhanced Molecular Imaging of Live Cells on Gold Substrates

2011 ◽  
Vol 50 (20) ◽  
pp. 4644-4648 ◽  
Author(s):  
Guosong Hong ◽  
Scott M. Tabakman ◽  
Kevin Welsher ◽  
Zhuo Chen ◽  
Joshua T. Robinson ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Near Infrared ◽  
Live Cells ◽  
Near Infrared Fluorescence ◽  
Gold Substrates

scholarly journals New near-infrared rhodamine dyes with large Stokes shifts for sensitive sensing of intracellular pH changes and fluctuations

2018 ◽  
Vol 54 (55) ◽  
pp. 7625-7628 ◽  
Author(s):  
Yibin Zhang ◽  
Shuai Xia ◽  
Mingxi Fang ◽  
Wafa Mazi ◽  
Yanbo Zeng ◽  
...  
Keyword(s):  
Intracellular Ph ◽  
Near Infrared ◽  
Sensitive Detection ◽  
Amine Group ◽  
Ph Changes ◽  
Stokes Shifts ◽  
Rhodamine Dyes

New near-infrared rhodamine dyes with large Stokes shifts were developed and applied for sensitive detection of cellular pH changes and fluctuations by incorporating an additional amine group with fused rings.

Turn-on visible and ratiometric near-infrared fluorescent probes for distinction endogenous esterases and chymotrypsins in live cells

2020 ◽  
Vol 306 ◽  
pp. 127567 ◽  
Author(s):  
Jiemin Wang ◽  
Zhidong Teng ◽  
Ting Cao ◽  
Jing Qian ◽  
Lei Zheng ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Near Infrared ◽  
Live Cells ◽  
Turn On

scholarly journals A Water-soluble Near-infrared Fluorescent Probe for Cysteine/Homocysteine and Its Application in Live Cells and Mice

2020 ◽  
Vol 36 (9) ◽  
pp. 1053-1057
Author(s):  
Yuhui MEN ◽  
Xiaomin ZHOU ◽  
Zhijie YAN ◽  
Linqiang NIU ◽  
Yang LUO ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Near Infrared ◽  
Water Soluble ◽  
Live Cells

Two novel two-photon excited fluorescent pH probes based on the A-π-D-π-A system for intracellular pH mapping

2017 ◽  
Vol 136 ◽  
pp. 807-816 ◽  
Author(s):  
Shuyi Chen ◽  
Meng Zhao ◽  
Jian Su ◽  
Qiong Zhang ◽  
Xiaohe Tian ◽  
...  
Keyword(s):  
Intracellular Ph ◽  
Two Photon ◽  
Ph Probes

scholarly journals A Highly Sensitive Fluorogenic Probe for Imaging Glycoproteins and Mucine Activity in Live Cells in the Near-Infrared Region

2018 ◽  
Vol 24 (24) ◽  
pp. 6344-6348 ◽  
Author(s):  
Cecilia Samaniego Lopez ◽  
Jimena Hebe Martínez ◽  
María Laura Uhrig ◽  
Federico Coluccio Leskow ◽  
Carla Cecilia Spagnuolo
Keyword(s):  
Near Infrared ◽  
Infrared Region ◽  
Live Cells ◽  
Fluorogenic Probe ◽  
Highly Sensitive ◽  
Near Infrared Region
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