Label free fluorescence turn-on detection of polynucleotide kinase activity with a perylene probe

2012 ◽  
Vol 48 (63) ◽  
pp. 7862 ◽  
Author(s):  
Huping Jiao ◽  
Bin Wang ◽  
Jian Chen ◽  
Dongli Liao ◽  
Wenying Li ◽  
...  
Keyword(s):  
Kinase Activity ◽  
Label Free ◽  
Turn On ◽  
Polynucleotide Kinase ◽  
Fluorescence Turn On

One-strand oligonucleotide probe for fluorescent label-free “turn-on” detection of T4 polynucleotide kinase activity and its inhibition

The Analyst ◽  
2015 ◽  
Vol 140 (16) ◽  
pp. 5650-5655 ◽  
Author(s):  
Fu Zhou ◽  
Guangfeng Wang ◽  
Dongmin Shi ◽  
Yue Sun ◽  
Liang Sha ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Kinase Activity ◽  
Oligonucleotide Probe ◽  
Thioflavin T ◽  
Fluorescent Label ◽  
Label Free ◽  
Molecular Rotor ◽  
Turn On ◽  
Polynucleotide Kinase ◽  
G Quadruplex

Thioflavin T (ThT), as one of the most exciting fluorogenic molecules, boasts the “molecular-rotor” ability to induce DNA sequences containing guanine repeats to fold into G-quadruplex structures.

Label-free fluorescence assay coupled exonuclease reaction and SYBR Green I for the detection of T4 polynucleotide kinase activity

2020 ◽  
Vol 12 (6) ◽  
pp. 807-812
Author(s):  
Xu Wu ◽  
Shuyi He ◽  
Julia Xiaojun Zhao
Keyword(s):  
Kinase Activity ◽  
Sybr Green ◽  
Fluorescence Assay ◽  
Sybr Green I ◽  
Label Free ◽  
Cleavage Reaction ◽  
Polynucleotide Kinase ◽  
T4 Polynucleotide Kinase

A sensitive label-free fluorescence assay for monitoring T4 polynucleotide kinase (T4 PNK) activity and inhibition was developed based on a coupled λ exonuclease cleavage reaction and SYBR Green I.

Polymer-Templated Perylene-Probe Noncovalent Self-Assembly: A New Strategy for Label-Free Ultrasensitive Fluorescence Turn-On Biosensing

2013 ◽  
Vol 19 (38) ◽  
pp. 12846-12852 ◽  
Author(s):  
Yan Wang ◽  
Jian Chen ◽  
Huping Jiao ◽  
Yang Chen ◽  
Wenying Li ◽  
...  
Keyword(s):  
Self Assembly ◽  
Label Free ◽  
Turn On ◽  
New Strategy ◽  
Fluorescence Turn On

scholarly journals Design of a Fluorescence Turn-on and Label-free Aptasensor Using the Intrinsic Quenching Power of G-Quadruplex to AMT

2020 ◽  
Vol 36 (8) ◽  
pp. 965-970
Author(s):  
Dandan WANG ◽  
Fenghua GENG ◽  
Yongxiang WANG ◽  
Yu MA ◽  
Guixin LI ◽  
...  
Keyword(s):  
Label Free ◽  
Turn On ◽  
G Quadruplex ◽  
Fluorescence Turn On

A label-free fluorescence turn-on sensor for rapid detection of cysteine

Talanta ◽  
2015 ◽  
Vol 138 ◽  
pp. 144-148 ◽  
Author(s):  
Xia Chen ◽  
Hongli Liu ◽  
Chen Wang ◽  
Hui Hu ◽  
Yuhui Wang ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Label Free ◽  
Turn On ◽  
Fluorescence Turn On

Nucleic acid-controlled quantum dots aggregation: A label-free fluorescence turn-on strategy for alkaline phosphatase detection

Talanta ◽  
2017 ◽  
Vol 169 ◽  
pp. 64-69 ◽  
Author(s):  
Zhenzhen Hu ◽  
Jian Chen ◽  
Yongxin Li ◽  
Yan Wang ◽  
Qingfeng Zhang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Alkaline Phosphatase ◽  
Nucleic Acid ◽  
Label Free ◽  
Turn On ◽  
Fluorescence Turn On

Improved Detection of Hg2+ in Aqueous Solution with High Sensitivity and Selectivity by Using Mercury-Specific DNA, Sybr Green I and Gold Nanoparticles

2011 ◽  
Vol 239-242 ◽  
pp. 934-939
Author(s):  
Hui Xu ◽  
Shuli Gao ◽  
Jian Nong Chen ◽  
Quan Wen Liu
Keyword(s):  
Gold Nanoparticles ◽  
High Sensitivity ◽  
Sybr Green ◽  
Sybr Green I ◽  
Fluorescence Signal ◽  
Potassium Ions ◽  
Label Free ◽  
Turn On ◽  
Sensitivity And Selectivity ◽  
Fluorescence Turn On

We report a label-free, fast, fluorescence turn on assay for Hg2+detecton by using mercury-specific DNA (MSD), Sybr Green I (SG) and gold nanoparticles (AuNPs). SG efficiently discriminates MSD and MSD/Hg2+complex. The addition of gold nanoparticle decreases the background fluorescence signal further for MSD. The fluorescence intensity of MSD/Hg2+complex keeps constant after addition of AuNPs. This property improves the signal-to-background ratio and decreases the detection limitation further. In addition, the method shows improved selectivity compared with that in the absence of AuNPs. This strategy could be applied to the detection of potassium ions and showed good generality.

Sign in / Sign up

Export Citation Format

Share Document