Shapes of supramolecular cages by ion mobility mass spectrometry

2012 ◽  
Vol 48 (37) ◽  
pp. 4423-4425 ◽  
Author(s):  
Jakub Ujma ◽  
Martin De Cecco ◽  
Oleg Chepelin ◽  
Hannah Levene ◽  
Chris Moffat ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Ion Mobility ◽  
Supramolecular Complexes ◽  
Ion Mobility Mass Spectrometry ◽  
Structural Differences ◽  
Self Assembled ◽  
The Stability

Ion mobility mass spectrometry distinguishes between isobaric supramolecular complexes, revealing subtle structural differences and their effect on the stability of self assembled architectures.

scholarly journals Correlating glycoforms of DC-SIGN with stability using a combination of enzymatic digestion and ion mobility MS

2020 ◽  
Author(s):  
Hsin-Yung Yen ◽  
Idlir Liko ◽  
Joseph Gault ◽  
Di Wu ◽  
Weston B. Struwe ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Phase ◽  
Ion Mobility ◽  
Collision Cross Section ◽  
Enzymatic Digestion ◽  
Ion Mobility Mass Spectrometry ◽  
Structural Detail ◽  
The Core ◽  
Glycosylation Sites ◽  
The Stability

AbstractThe immune scavenger protein DC-SIGN interacts with glycosylated proteins and has a putative role in facilitating viral infection. How these recognition events take place with different viruses is not clear and the effects of glycosylation on the folding and stability of DC-SIGN have not been reported. Here, we develop and apply a mass spectrometry-based approach to both uncover and characterise the effects of O-glycans on the stability of DC-SIGN. We first quantify the Core 1 & 2 O-glycan structures on the carbohydrate recognition and extracellular domains of the protein via sequential exoglycosidase sequencing. We then use ion mobility mass spectrometry to show how specific O-glycans, and/or single monosaccharide substitutions, alter both the overall collision cross section and the gas-phase stability of the glycoprotein isoforms of DC-SIGN. We find that rather than the mass or length of glycoprotein modifications, the stability of DC-SIGN is better correlated with the number of glycosylation sites. Collectively, our results exemplify a combined multi-dimensional MS approach, proficient in evaluating protein stability in response to both glycoprotein macro- and micro-heterogeneity and adding structural detail to the infection enhancer DC-SIGN.

Probing the Stability of Insulin Oligomers Using Electrospray Ionization Ion Mobility Mass Spectrometry

2015 ◽  
Vol 21 (6) ◽  
pp. 759-774 ◽  
Author(s):  
Uday Kumar Boga Raja ◽  
Srilakshmi Injeti ◽  
Tiffany Culver ◽  
Jacob W. McCabe ◽  
Laurence A. Angel
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Ion Mobility ◽  
Ion Mobility Mass Spectrometry ◽  
The Stability

scholarly journals Collision cross‐section analysis of self‐assembled metallomacrocycle isomers and isobars via ion mobility mass spectrometry

2020 ◽  
Vol 34 (S2) ◽  
Author(s):  
Kevin J. Endres ◽  
Kevin Barthelmes ◽  
Andreas Winter ◽  
Robert Antolovich ◽  
Ulrich S. Schubert ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cross Section ◽  
Ion Mobility ◽  
Collision Cross Section ◽  
Ion Mobility Mass Spectrometry ◽  
Self Assembled ◽  
Section Analysis

scholarly journals A Combined Approach of NMR and Mass Spectrometry Techniques Applied to the α-Cyclodextrin/Moringin Complex for a Novel Bioactive Formulation †

Molecules ◽  
2018 ◽  
Vol 23 (7) ◽  
pp. 1714 ◽  
Author(s):  
David Mathiron ◽  
Renato Iori ◽  
Serge Pilard ◽  
Thangavelu Soundara Rajan ◽  
David Landy ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Ion Mobility ◽  
Biological Activities ◽  
Proton Nuclear Magnetic Resonance ◽  
Ion Mobility Mass Spectrometry ◽  
Enzymatic Conversion ◽  
Combined Approach ◽  
Bactericidal Effects ◽  
Nmr Diffusion ◽  
The Stability

Moringin, obtained via enzymatic conversion of the glucosinolate precursor glucomoringin, is an uncommon member of the isothiocyanate class, and has been proven to possess a broad range of biological activities such as antitumor activity, protection against neurodegenerative disorders and bactericidal effects. Since moringin is weakly soluble in water and unstable in aqueous medium, cyclodextrins (CDs) were considered for the development of a new moringin formulation, with a view to improving its solubility and stability in aqueous solution for use as an anti-inflammatory. A combined structural study using proton nuclear magnetic resonance (1H-NMR), diffusion-ordered spectroscopy (DOSY) and ion mobility mass spectrometry (IM-MS) is reported, highlighting the formation of a 1:1 α-CD/moringin inclusion complex. The association constant K was determined (1300 M−1 at 300 K). Completion of the structural characterization was performed by T-ROESY and MS/MS experiments, which evidenced the mode of penetration of moringin into α-CD. Finally, the “chaperone-like” properties of α-CD with respect to the stability of moringin have been highlighted.

Rapid Diagnosis of Parkinson’s Disease from Sebum using Paper Spray Ionisation Ion Mobility Mass Spectrometry

2020 ◽  
Author(s):  
Depanjan Sarkar ◽  
Drupad Trivedi ◽  
Eleanor Sinclair ◽  
Sze Hway Lim ◽  
Caitlin Walton-Doyle ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Ion Mobility ◽  
Neurodegenerative Disorder ◽  
Treatment Options ◽  
Ion Mobility Mass Spectrometry ◽  
Paper Spray ◽  
Molecular Features ◽  
Validation Set

Parkinson’s disease (PD) is the second most common neurodegenerative disorder for which identification of robust biomarkers to complement clinical PD diagnosis would accelerate treatment options and help to stratify disease progression. Here we demonstrate the use of paper spray ionisation coupled with ion mobility mass spectrometry (PSI IM-MS) to determine diagnostic molecular features of PD in sebum. PSI IM-MS was performed directly from skin swabs, collected from 34 people with PD and 30 matched control subjects as a training set and a further 91 samples from 5 different collection sites as a validation set. PSI IM-MS elucidates ~ 4200 features from each individual and we report two classes of lipids (namely phosphatidylcholine and cardiolipin) that differ significantly in the sebum of people with PD. Putative metabolite annotations are obtained using tandem mass spectrometry experiments combined with accurate mass measurements. Sample preparation and PSI IM-MS analysis and diagnosis can be performed ~5 minutes per sample offering a new route to for rapid and inexpensive confirmatory diagnosis of this disease.

P‐97: Separation of Isomeric OLED Materials Using Ion Mobility‐Mass Spectrometry (IM‐MS)

2021 ◽  
Vol 52 (1) ◽  
pp. 1444-1447
Author(s):  
Hirotaka Shioji ◽  
Azusa Uematsu ◽  
Motoshi Onoda ◽  
Keiko Matsuda ◽  
Keisuke Sawada ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Ion Mobility ◽  
Ion Mobility Mass Spectrometry
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