Real-time electrochemical detection of pathogen DNA using electrostatic interaction of a redox probe

The Analyst ◽  
2013 ◽  
Vol 138 (3) ◽  
pp. 907-915 ◽  
Author(s):  
Minhaz Uddin Ahmed ◽  
Sharifun Nahar ◽  
Mohammadali Safavieh ◽  
Mohammed Zourob
Keyword(s):  
Real Time ◽  
Electrochemical Detection ◽  
Electrostatic Interaction ◽  
Redox Probe ◽  
Pathogen Dna

A nanostructured microfluidic device for plasmonic-assisted electrochemical detection of hydrogen peroxide released from cancer cells

Nanoscale ◽  
2021 ◽  
Author(s):  
Carolina del Real Mata ◽  
Roozbeh Siavash Moakhar ◽  
Sayed Iman Isaac Hosseini ◽  
Mahsa Jalali ◽  
Sara Mahshid
Keyword(s):  
Hydrogen Peroxide ◽  
Cancer Cells ◽  
Real Time ◽  
Electrochemical Detection ◽  
Microfluidic Device ◽  
Cancer Biomarker ◽  
Cancer Diagnostics ◽  
Liquid Biopsies ◽  
Non Invasive

Non-invasive liquid biopsies offer hope for a rapid, risk-free, real-time glimpse into cancer diagnostics. Recently, hydrogen peroxide (H2O2) is identified as a cancer biomarker due to continued release from cancer...

A fully integrated passive microfluidic Lab-on-a-Chip for real-time electrochemical detection of ammonium: Sewage applications

2019 ◽  
Vol 653 ◽  
pp. 1223-1230 ◽  
Author(s):  
J. Gallardo-Gonzalez ◽  
A. Baraket ◽  
S. Boudjaoui ◽  
T. Metzner ◽  
F. Hauser ◽  
...  
Keyword(s):  
Real Time ◽  
Electrochemical Detection ◽  
Lab On A Chip ◽  
Fully Integrated

Real-Time Electrochemical Detection ofPseudomonas aeruginosaPhenazine Metabolites Using Transparent Carbon Ultramicroelectrode Arrays

ACS Sensors ◽  
2018 ◽  
Vol 4 (1) ◽  
pp. 170-179 ◽  
Author(s):  
Olja Simoska ◽  
Marta Sans ◽  
Mignon D. Fitzpatrick ◽  
Christopher M. Crittenden ◽  
Livia S. Eberlin ◽  
...  
Keyword(s):  
Real Time ◽  
Electrochemical Detection

Facile synthesis of ultrathin NiSnO3 nanoparticles for enhanced electrochemical detection of an antibiotic drug in water bodies and biological samples

2020 ◽  
Vol 44 (25) ◽  
pp. 10604-10612
Author(s):  
Muthaiah Annalakshmi ◽  
Subbarayan Sumithra ◽  
Shen-Ming Chen ◽  
Tse-Wei Chen ◽  
Xuei-Hong Zheng
Keyword(s):  
Real Time ◽  
Electrochemical Detection ◽  
Biological Samples ◽  
Facile Synthesis ◽  
Research Attention ◽  
Living Things ◽  
Antibiotic Drugs ◽  
Highly Sensitive ◽  
Antibiotic Drug ◽  
Significant Research

The highly sensitive real-time detection of antibiotic drugs (nitrofurantoin; NFT) has drawn significant research attention due to the extensive use of antibiotics, which may cause serious threats to environment as well as living things.

scholarly journals Real-time electrochemical detection of extracellular nitric oxide in tobacco cells exposed to cryptogein, an elicitor of defence responses

2008 ◽  
Vol 59 (12) ◽  
pp. 3407-3414 ◽  
Author(s):  
Angélique Besson-Bard ◽  
Sophie Griveau ◽  
Fethi Bedioui ◽  
David Wendehenne
Keyword(s):  
Nitric Oxide ◽  
Real Time ◽  
Electrochemical Detection ◽  
Tobacco Cells ◽  
Defence Responses

scholarly journals Simultaneous One-Tube Quantification of Host and Pathogen DNA with Real-Time Polymerase Chain Reaction

2002 ◽  
Vol 92 (1) ◽  
pp. 112-116 ◽  
Author(s):  
L. M. Winton ◽  
J. K. Stone ◽  
L. S. Watrud ◽  
E. M. Hansen
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Fluorescent Dyes ◽  
Quantitative Polymerase Chain Reaction ◽  
Positive Control ◽  
Douglas Fir ◽  
Chain Reaction ◽  
Taqman Probes ◽  
Polymerase Chain ◽  
Pathogen Dna

Phaeocryptopus gaeumannii is a widespread foliar parasite of Douglas-fir. Although normally innocuous, the fungus also causes the defoliating disease Swiss needle cast in heavily infected needles. The extent of P. gaeumannii colonization in Douglas-fir foliage was estimated with real-time quantitative polymerase chain reaction (PCR) using TaqMan chemistry. In order to derive a normalized expression of colonization, both pathogen and host DNA were simultaneously amplified but individually detected by species-specific primers and TaqMan probes labeled with different fluorescent dyes. Detection of host DNA additionally provided an endogenous reference that served as both an internal positive control and adjusted for variation introduced by sample-to-sample differences in DNA extraction and PCR efficiencies. The genes employed for designing the TaqMan probes and primers were β-tubulin for the pathogen and a LEAFY/FLORICAULA-like gene involved in floral development for the tree host. Both probe/primer sets exhibited high precision and reproducibility over a linear range of 4 orders of magnitude. This eliminated the need to analyze samples in multiple dilutions when comparing lightly with heavily infected needles. Quantification of the fungus within needles was successful as early as 1 month after initial infection. Real-time PCR is the only method currently available to quantify P. gaeumannii colonization early in the first year of the colonization process.

scholarly journals Evaluation of pathogen detection from clinical samples by real-time polymerase chain reaction using a sepsis pathogen DNA detection kit

Critical Care ◽  
2010 ◽  
Vol 14 (4) ◽  
pp. R159 ◽  
Author(s):  
Katsunori Yanagihara ◽  
Yuko Kitagawa ◽  
Masao Tomonaga ◽  
Kunihiro Tsukasaki ◽  
Shigeru Kohno ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Pathogen Detection ◽  
Dna Detection ◽  
Clinical Samples ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Pathogen Dna

Real-time PCR microfluidic devices with concurrent electrochemical detection

2009 ◽  
Vol 24 (7) ◽  
pp. 2131-2136 ◽  
Author(s):  
Teh Huey Fang ◽  
Naveen Ramalingam ◽  
Dong Xian-Dui ◽  
Tan Swee Ngin ◽  
Zeng Xianting ◽  
...  
Keyword(s):  
Real Time ◽  
Electrochemical Detection ◽  
Real Time Pcr ◽  
Microfluidic Devices
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