Fabrication of micro free-flow electrophoresis chip by photocurable monomer binding microfabrication technique for continuous separation of proteins and their numerical simulation

The Analyst ◽  
2012 ◽  
Vol 137 (19) ◽  
pp. 4482 ◽  
Author(s):  
Hui Ding ◽  
Xiaoqiong Li ◽  
Xuefei Lv ◽  
Jiandong Xu ◽  
Xin Sun ◽  
...  
Keyword(s):  
Numerical Simulation ◽  
Free Flow ◽  
Continuous Separation ◽  
Microfabrication Technique ◽  
Separation Of Proteins ◽  
Micro Free Flow Electrophoresis

Continuous Separation of Proteins by Free-Flow Zone Electrophoresis

1989 ◽  
Vol 93 (9) ◽  
pp. 1042-1046 ◽  
Author(s):  
Sunil Nath ◽  
Horst Schütte ◽  
Helmut Hustedt ◽  
Wolf-Dieter Deckwer
Keyword(s):  
Free Flow ◽  
Flow Zone ◽  
Continuous Separation ◽  
Zone Electrophoresis ◽  
Separation Of Proteins

scholarly journals Micro free flow electrophoresis

Lab on a Chip ◽  
2018 ◽  
Vol 18 (1) ◽  
pp. 27-40 ◽  
Author(s):  
Alexander C. Johnson ◽  
Michael T. Bowser
Keyword(s):  
Online Monitoring ◽  
Free Flow ◽  
Separation Technique ◽  
Continuous Separation ◽  
Monitoring Applications ◽  
Micro Free Flow Electrophoresis

This review focuses on the fabrication methods, theory, and applications of micro free flow electrophoresis (μFFE), a continuous separation technique particularly well suited for microscale purifications and online monitoring applications.

Nonaqueous Micro Free-Flow Electrophoresis for Continuous Separation of Reaction Mixtures in Organic Media

2019 ◽  
Author(s):  
Benjamin M. Rudisch ◽  
Simon A. Pfeiffer ◽  
David Geissler ◽  
Elisabeth Speckmeier ◽  
Andrea A. Robitzki ◽  
...  
Keyword(s):  
Free Flow ◽  
Organic Media ◽  
Continuous Separation ◽  
Micro Free Flow Electrophoresis

Optimizing Band Width and Resolution in Micro-Free Flow Electrophoresis

2006 ◽  
Vol 78 (24) ◽  
pp. 8236-8244 ◽  
Author(s):  
Bryan R. Fonslow ◽  
Michael T. Bowser
Keyword(s):  
Free Flow ◽  
Band Width ◽  
Micro Free Flow Electrophoresis

Free Flow Isoelectric Focusing in a Microfluidic Device

2014 ◽  
Author(s):  
Kisoo Yoo ◽  
Prashanta Dutta ◽  
Jin Liu
Keyword(s):  
Mathematical Model ◽  
Isoelectric Focusing ◽  
Microfluidic Device ◽  
Microfluidic Chip ◽  
Cardiac Troponin ◽  
Troponin I ◽  
Free Flow ◽  
Charged Species ◽  
Simulation Results ◽  
Separation Of Proteins

In recent years, there are growing interests in the use of free flow isoelectric focusing (FFIEF). In FFIEF, a thin sheath of laminar flow is introduced perpendicular to the direction of the applied electric field for continuous separation of proteins and charged species. This technique is especially useful in microfluidic device since the electrophoretically separated bands do not have to be mobilized for detection or further analysis. In this study, a mathematical model is developed to simulate free flow isoelectric process in microfluidic devices considering electroneutrality and incompressibility of electrolytes. Our mathematical model is based on mass, momentum and charge conservation equations. A finite volume based numerical scheme is implemented to simulate two dimensional FFIEF in a microfluidic chip. Simulation results indicate that pH gradient forms as samples flow downstream and proteins can be separated effectively using this technique. A new design of microfluidic chip is proposed for separation for cardiac troponin I from serum albumin using FFIEF technique.

Continuous Separation of Proteins by Multichannel Flow Electrophoresis

1996 ◽  
Vol 31 (10) ◽  
pp. 1426-1442 ◽  
Author(s):  
Zheng Liu ◽  
Zheng Huang ◽  
Jinyang Chong ◽  
Huan Yang ◽  
Fuxin Ding ◽  
...  
Keyword(s):  
Continuous Separation ◽  
Separation Of Proteins

scholarly journals Micro free-flow electrophoresis: theory and applications

2009 ◽  
Vol 394 (1) ◽  
pp. 187-198 ◽  
Author(s):  
Ryan T. Turgeon ◽  
Michael T. Bowser
Keyword(s):  
Free Flow ◽  
Micro Free Flow Electrophoresis

scholarly journals Improving sensitivity in micro-free flow electrophoresis using signal averaging

2009 ◽  
Vol 30 (8) ◽  
pp. 1342-1348 ◽  
Author(s):  
Ryan T. Turgeon ◽  
Michael T. Bowser
Keyword(s):  
Free Flow ◽  
Signal Averaging ◽  
Micro Free Flow Electrophoresis
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