Sensitive sandwich ELISA based on a gold nanoparticle layer for cancer detection

The Analyst ◽  
2012 ◽  
Vol 137 (8) ◽  
pp. 1779 ◽  
Author(s):  
Feng Zhou ◽  
Mengmeng Wang ◽  
Lin Yuan ◽  
Zhenping Cheng ◽  
Zhaoqiang Wu ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Cancer Detection ◽  
Sandwich Elisa ◽  
Nanoparticle Layer

Gold Nanoparticle Layer: A Promising Platform for Ultra-Sensitive Cancer Detection

Langmuir ◽  
2011 ◽  
Vol 27 (6) ◽  
pp. 2155-2158 ◽  
Author(s):  
Feng Zhou ◽  
Lin Yuan ◽  
Hongwei Wang ◽  
Dan Li ◽  
Hong Chen
Keyword(s):  
Gold Nanoparticle ◽  
Cancer Detection ◽  
Nanoparticle Layer

scholarly journals Composite Structure Based on Gold-Nanoparticle Layer and HMM for Surface-Enhanced Raman Spectroscopy Analysis

Nanomaterials ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 587
Author(s):  
Zirui Wang ◽  
Yanyan Huo ◽  
Tingyin Ning ◽  
Runcheng Liu ◽  
Zhipeng Zha ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Surface Plasmon ◽  
Composite Structure ◽  
Surface Enhanced Raman Spectroscopy ◽  
Localized Surface Plasmon ◽  
Sers Substrate ◽  
Nanoparticle Layer ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Plasmon Polaritons

Hyperbolic metamaterials (HMMs), supporting surface plasmon polaritons (SPPs), and highly confined bulk plasmon polaritons (BPPs) possess promising potential for application as surface-enhanced Raman scattering (SERS) substrates. In the present study, a composite SERS substrate based on a multilayer HMM and gold-nanoparticle (Au-NP) layer was fabricated. A strong electromagnetic field was generated at the nanogaps of the Au NPs under the coupling between localized surface plasmon resonance (LSPR) and a BPP. Additionally, a simulation of the composite structure was assessed using COMSOL; the results complied with those achieved through experiments: the SERS performance was enhanced, while the enhancing rate was downregulated, with the extension of the HMM periods. Furthermore, this structure exhibited high detection performance. During the experiments, rhodamine 6G (R6G) and malachite green (MG) acted as the probe molecules, and the limits of detection of the SERS substrate reached 10−10 and 10−8 M for R6G and MG, respectively. Moreover, the composite structure demonstrated prominent reproducibility and stability. The mentioned promising results reveal that the composite structure could have extensive applications, such as in biosensors and food safety inspection.

scholarly journals Combinatorial investigation of the isolated nanoparticle to coalescent layer transition in a gradient sputtered gold nanoparticle layer on top of polystyrene

2006 ◽  
Vol 88 (2) ◽  
pp. 021910 ◽  
Author(s):  
S. V. Roth ◽  
H. Walter ◽  
M. Burghammer ◽  
C. Riekel ◽  
B. Lengeler ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Layer Transition ◽  
Nanoparticle Layer

Dimeric Gold Nanoparticle Assemblies as Tags for SERS-Based Cancer Detection

2013 ◽  
Vol 2 (10) ◽  
pp. 1370-1376 ◽  
Author(s):  
A. Swarnapali D. S. Indrasekara ◽  
Bryan J. Paladini ◽  
Dominik J. Naczynski ◽  
Valentin Starovoytov ◽  
Prabhas V. Moghe ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Cancer Detection ◽  
Nanoparticle Assemblies

scholarly journals Blocking-Free ELISA Using a Gold Nanoparticle Layer Coated Commercial Microwell Plate

Sensors ◽  
2018 ◽  
Vol 18 (10) ◽  
pp. 3537 ◽  
Author(s):  
Ruijia Huang ◽  
Ke Zhang ◽  
Guoshuai Zhu ◽  
Zhencheng Sun ◽  
Songliang He ◽  
...  
Keyword(s):  
Protein Adsorption ◽  
Gold Nanoparticle ◽  
Clinical Diagnosis ◽  
Protein Biomarkers ◽  
Negative Effects ◽  
Elisa Kit ◽  
Nanoparticle Layer ◽  
Time Required ◽  
Nonspecific Protein Adsorption ◽  
Microwell Plate

Enzyme-linked immunosorbent assays (ELISA) show extensive application in immunoassays, to detect and monitor protein biomarkers in clinical diagnosis. Nevertheless, the time required and its multiple steps limit its application. We take advantage of a polyethyleneimine (PEI) gold nanoparticle (GNP) coated microwell plate to perform blocking-free ELISA, in which no nonspecific protein adsorption appears on the GNP layer. If the PEI-GNP coated microwell plate and immobilization of captured antibodies on the plate are prepared in advance, such as using an ELISA kit, the whole ELISA process can be finished in less than 2 h. Meanwhile, we have ensured that the GNP layer can preserve the precision and good linearity of ELISA without causing negative effects on the plate.

Gold-Nanoparticle Layer Substrate Assisted Transmission-Mode Laser Desorption for Atmospheric Pressure Mass Spectrometry Imaging

2020 ◽  
Vol 12 (10) ◽  
pp. 1517-1523
Author(s):  
Jae Young Kim ◽  
Heejin Lim ◽  
Sun Young Lee ◽  
Gwanjin Lee ◽  
Dong-Kwon Lim ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gold Nanoparticle ◽  
Atmospheric Pressure ◽  
Mass Spectrometry Imaging ◽  
Continuous Wave ◽  
Continuous Wave Laser ◽  
Fresh Tissue ◽  
Mode Laser ◽  
Nanoparticle Layer ◽  
Wave Laser

We demonstrate continuous-wave laser-based ambient mass spectrometry imaging with the use of a gold-nanoparticle layer substrate. When a fresh tissue slice is placed on a gold-nanoparticle layer substrate and irradiated with a 532-nm continuous-wave laser, the transmission-mode laser configuration provides precise desorption performance to facilitate mass spectrometry imaging. The subsequent ionization process with nonthermal atmospheric pressure plasma jets generates sufficient amounts of molecular ions. By using this method, micrometer-spatial-resolution mass spectrometry imaging of humid tissues can be obtained. The gold-nanoparticle layer substrates can be prepared and stored in advance of the experiment, resulting in simplified specimen preparation and an advantage in faster preparing of fresh tissue specimen for analysis.

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