Effect of monovalent cations (Li+, Na+, K+, Cs+) on self-assembly of thiol-modified double-stranded and single-stranded DNA on gold electrode

The Analyst ◽  
2012 ◽  
Vol 137 (7) ◽  
pp. 1680 ◽  
Author(s):  
Zhiguo Li ◽  
Tianxing Niu ◽  
Zhenjiang Zhang ◽  
Guiying Feng ◽  
Shuping Bi
Keyword(s):  
Self Assembly ◽  
Gold Electrode ◽  
Monovalent Cations ◽  
Single Stranded Dna

Electrochemical Characteristics of Aptasensor Based on MEMS Thin-Film Gold Electrode

2013 ◽  
Vol 562-565 ◽  
pp. 441-445
Author(s):  
Yan Xia Yan ◽  
Li Ying Jiang ◽  
Fen Fen Wang ◽  
Yan Zhang ◽  
Jie Hu
Keyword(s):  
Thin Film ◽  
Self Assembly ◽  
Gold Electrode ◽  
Ac Impedance ◽  
Optimum Concentration ◽  
Electrochemical Characteristics ◽  
Universal Method

A new fabrication methods of thin-film gold electrode is reported. Electrochemical characteristics of self-assembly aptasensor based on MEMS thin-film gold electrode has been studied by lots of experiments, characteristics include immobilizing time, AC impedance, CV curves. Experiments indicate immobilizing time of DNA-SH on gold electrode is more than 15 hours. CV curves and AC impedance of MEMS thin-film gold electrode show the same conclusion that 20μm/L DNA is optimum concentration when DNA is immobilized on gold electrode. Those results present a potential universal method for other aptasensors.

scholarly journals Linker-mediated self-assembly of mobile DNA-coated colloids

2020 ◽  
Vol 6 (21) ◽  
pp. eaaz6921 ◽  
Author(s):  
Xiuyang Xia ◽  
Hao Hu ◽  
Massimo Pica Ciamarra ◽  
Ran Ni
Keyword(s):  
Self Assembly ◽  
Effective Interaction ◽  
Mean Field Theory ◽  
Mean Field ◽  
Mobile Dna ◽  
Complementary Sequence ◽  
Single Stranded Dna ◽  
Construction Methods ◽  
Free Dna ◽  
Innovative System

Developing construction methods of materials tailored for given applications with absolute control over building block placement poses an immense challenge. DNA-coated colloids offer the possibility of realising programmable self-assembly, which, in principle, can assemble almost any structure in equilibrium, but remains challenging experimentally. Here, we propose an innovative system of linker-mediated mobile DNA-coated colloids (mDNACCs), in which mDNACCs are bridged by the free DNA linkers in solution, whose two single-stranded DNA tails can bind with specific single-stranded DNA receptors of complementary sequence coated on colloids. We formulate a mean-field theory efficiently calculating the effective interaction between mDNACCs, where the entropy of DNA linkers plays a nontrivial role. Particularly, when the binding between free DNA linkers in solution and the corresponding receptors on mDNACCs is strong, the linker-mediated colloidal interaction is determined by the linker entropy depending on the linker concentration.

Oligomer Length Defines the Self-Assembly of Single-Stranded DNA–Collagen Complex Fibers

2019 ◽  
Vol 6 (1) ◽  
pp. 213-218 ◽  
Author(s):  
Bryan D. James ◽  
Sophia Saenz ◽  
Anastacia van Gent ◽  
Josephine B. Allen
Keyword(s):  
Self Assembly ◽  
The Self ◽  
Single Stranded Dna

Catalytic Oxidation of NADH on Gold Electrode Modified by Layer-by- Layer Self-Assembly of Thermostable Diaphorase and Redox Polymer

2011 ◽  
Vol 675-677 ◽  
pp. 231-234 ◽  
Author(s):  
Wen Juan Zheng ◽  
Hai Tao Zheng ◽  
Tao Sun ◽  
Pu Liu ◽  
Shinichiro Suye
Keyword(s):  
Self Assembly ◽  
Multilayer Structure ◽  
Gold Electrode ◽  
Multilayer Film ◽  
Water Soluble ◽  
Current Response ◽  
Layer By Layer ◽  
Redox Polymer ◽  
Relative Response ◽  
Reduced Nicotinamide Adenine Dinucleotide

A redox polymer, poly(ethylenimine)ferrocene (PEI-Fc) was synthesized by attaching ferrocene groups to the backbone of water soluble poly(ethylenimine), and multilayer film in nanoscale was assembled on gold electrode by alternate layer-by-layer adsorption (LBL) of the positively charged PEI-Fc and the negatively charged thermostable diaphorase (DI) from B.Stearothermophilus. The LBL process was monitored and analyzed by quartz crystal microbalance (QCM) technique, which confirmed the formation of the multilayer structure. The electrochemical oxidation of coenzyme (reduced nicotinamide adenine dinucleotide, NADH) was observed on the electrode fabricated with PEI-Fc/DI multilayer film, and the influence of layer number on current response was investigated. The modified electrode retained ca. 65% relative response after storage in buffer for two months and 50% relative response after incubation at 80 °C for 3 min, which inferred that the multilayer structure was unique stable. A NAD-dependent glucose-6-phosphate dehydrogenase (G6PDH) was also immobilized via the same LBL technique, and electrode modified with PEI-Fc/DI/G6PDH film exhibited current response to glucose-6- phosphate in the presence of free NAD+.

An electrochemical biosensor based on single-stranded DNA modified gold electrode for acrylamide determination

2016 ◽  
Vol 224 ◽  
pp. 22-30 ◽  
Author(s):  
Shan Huang ◽  
Shuangyan Lu ◽  
Chusheng Huang ◽  
Jiarong Sheng ◽  
Lixia Zhang ◽  
...  
Keyword(s):  
Gold Electrode ◽  
Electrochemical Biosensor ◽  
Single Stranded Dna

Evidence of Impurities in Thiolated Single-Stranded DNA Oligomers and Their Effect on DNA Self-Assembly on Gold

Langmuir ◽  
2005 ◽  
Vol 21 (11) ◽  
pp. 5134-5141 ◽  
Author(s):  
Chi-Ying Lee ◽  
Heather E. Canavan ◽  
Lara J. Gamble ◽  
David G. Castner
Keyword(s):  
Self Assembly ◽  
Single Stranded Dna ◽  
Dna Oligomers
Sign in / Sign up

Export Citation Format

Share Document