Platinum nanoparticle-facilitated reflective surfaces for non-contact temperature control in microfluidic devices for PCR amplification

Lab on a Chip ◽  
2012 ◽  
Vol 12 (1) ◽  
pp. 127-132 ◽  
Author(s):  
Daniel C. Leslie ◽  
Erkin Seker ◽  
Lindsay A. L. Bazydlo ◽  
Briony C. Strachan ◽  
James P. Landers
Sensors ◽  
2019 ◽  
Vol 19 (11) ◽  
pp. 2621 ◽  
Author(s):  
Junguk Ko ◽  
Jae-Chern Yoo

Polymerase chain reaction (PCR) and the visual inspection of fluorescent amplicons for detection are commonly used procedures in nucleic acid tests. However, it has been extremely challenging to incorporate PCR onto a lab-on-a-disc (PCR–LOD) as it involves controlling the complicated and precise heating steps during thermal cycling and the measurement of reagent temperature. Additionally, a non-contact temperature control system without any connecting attachments needs to be implemented to facilitate the rotation of the PCR–LOD. This study presents a non-contact temperature control system to integrate conventional PCR onto an LOD. The experimental results demonstrate that our proposed system provides one-stop detection capabilities for Salmonella with a stable PCR amplification in a single PCR–LOD.


Lab on a Chip ◽  
2003 ◽  
Vol 3 (1) ◽  
pp. 1 ◽  
Author(s):  
Rosanne M. Guijt ◽  
Arash Dodge ◽  
Gijs W. K. van Dedem ◽  
Nico F. de Rooij ◽  
Elisabeth Verpoorte

2002 ◽  
pp. 617-619
Author(s):  
A. Dodge ◽  
R. M. Guijt ◽  
G. W. K. van Dedem ◽  
N. F. de Rooij ◽  
E. Verpoorte

Soft Matter ◽  
2019 ◽  
Vol 15 (35) ◽  
pp. 6934-6937 ◽  
Author(s):  
Toshimitsu Kanai ◽  
Hiroki Nakai ◽  
Ayaka Yamada ◽  
Masafumi Fukuyama ◽  
David A. Weitz

We report a facile method for preparing monodisperse hybrid smart gel particles with various morphologies by using microfluidic devices.


Lab on a Chip ◽  
2004 ◽  
Vol 4 (5) ◽  
pp. 417 ◽  
Author(s):  
Andrew J. de Mello ◽  
Matthew Habgood ◽  
N. Llewellyn Lancaster ◽  
Tom Welton ◽  
Robert C. R. Wootton

Author(s):  
P.R. Swann ◽  
A.E. Lloyd

Figure 1 shows the design of a specimen stage used for the in situ observation of phase transformations in the temperature range between ambient and −160°C. The design has the following features a high degree of specimen stability during tilting linear tilt actuation about two orthogonal axes for accurate control of tilt angle read-out high angle tilt range for stereo work and habit plane determination simple, robust construction temperature control of better than ±0.5°C minimum thermal drift and transmission of vibration from the cooling system.


1981 ◽  
Vol 26 (8) ◽  
pp. 636-637
Author(s):  
James M. Lipton
Keyword(s):  

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