scholarly journals Silver metallation of hen egg white lysozyme: X-ray crystal structure and NMR studies

2011 ◽  
Vol 47 (46) ◽  
pp. 12479 ◽  
Author(s):  
Matthew J. Panzner ◽  
Stephanie M. Bilinovich ◽  
Wiley J. Youngs ◽  
Thomas C. Leeper
Keyword(s):  
Crystal Structure ◽  
Egg White ◽  
Hen Egg White Lysozyme ◽  
Nmr Studies ◽  
X Ray ◽  
Egg White Lysozyme ◽  
Hen Egg White ◽  
Hen Egg

Analysis of polycrystallinity in hen egg-white lysozyme using a pnCCD

2012 ◽  
Vol 45 (3) ◽  
pp. 517-522 ◽  
Author(s):  
Sebastian Send ◽  
Ali Abboud ◽  
Wolfram Leitenberger ◽  
Manfred S. Weiss ◽  
Robert Hartmann ◽  
...  
Keyword(s):  
Sample Surface ◽  
Egg White ◽  
Hen Egg White Lysozyme ◽  
X Ray ◽  
Lattice Constants ◽  
Egg White Lysozyme ◽  
Hen Egg White ◽  
Diffraction Patterns ◽  
Crystalline Domains ◽  
Hen Egg

A crystal of hen egg-white lysozyme was analyzed by means of energy-dispersive X-ray Laue diffraction with white synchrotron radiation at 2.7 Å resolution using a pnCCD detector. From Laue spots measured in a single exposure of the arbitrarily oriented crystal, the lattice constants of the tetragonal unit cell could be extracted with an accuracy of about 2.5%. Scanning across the sample surface, Laue images with split reflections were recorded at various positions. The corresponding diffraction patterns were generated by two crystalline domains with a tilt of about 1° relative to each other. The obtained results demonstrate the potential of the pnCCD for fast X-ray screening of crystals of macromolecules or proteins prior to conventional X-ray structure analysis. The described experiment can be automatized to quantitatively characterize imperfect single crystals or polycrystals.

Crystal distortion of monoclinic hen egg-white lysozyme crystals using X-ray digital topography

2014 ◽  
Vol 401 ◽  
pp. 238-241
Author(s):  
Kei Wako ◽  
Daiki Fujii ◽  
Shiro Tsukashima ◽  
Takeharu Kishi ◽  
Masaru Tachibana ◽  
...  
Keyword(s):  
Egg White ◽  
Hen Egg White Lysozyme ◽  
X Ray ◽  
Egg White Lysozyme ◽  
Hen Egg White ◽  
Lysozyme Crystals ◽  
Hen Egg

scholarly journals Hydrogen/deuterium exchange behavior in tetragonal hen egg-white lysozyme crystals affected by solution state

2020 ◽  
Vol 53 (3) ◽  
pp. 837-840
Author(s):  
Akiko Kita ◽  
Yukio Morimoto
Keyword(s):  
Egg White ◽  
Deuterium Exchange ◽  
Hydrogen Deuterium Exchange ◽  
Hen Egg White Lysozyme ◽  
Nmr Studies ◽  
Egg White Lysozyme ◽  
Hydrogen Deuterium ◽  
Hen Egg White ◽  
Lysozyme Crystals ◽  
Hen Egg

Neutron diffraction studies of hydrogen/deuterium-exchanged hen egg-white lysozyme were performed by a joint X-ray and neutron refinement to elucidate the hydrogen/deuterium exchange behavior. Large crystals for neutron work, consisting of molecules that were exchanged before crystallization, were obtained by repeatedly adding protein solution to the crystal batch using deuterated precipitant reagent. There are differences in hydrogen/deuterium exchange behavior compared with previous crystallographic or NMR studies, which could be due to intermolecular interactions in the crystal or to different lengths of exchange period.

The metalation of hen egg white lysozyme impacts protein stability as shown by ion mobility mass spectrometry, differential scanning calorimetry, and X-ray crystallography

2017 ◽  
Vol 53 (30) ◽  
pp. 4246-4249 ◽  
Author(s):  
Matthew P. Sullivan ◽  
Michael Groessl ◽  
Samuel M. Meier ◽  
Richard L. Kingston ◽  
David C. Goldstone ◽  
...  
Keyword(s):  
Ion Mobility ◽  
Egg White ◽  
Ion Mobility Mass Spectrometry ◽  
Hen Egg White Lysozyme ◽  
Scanning Calorimetry ◽  
X Ray ◽  
X Ray Crystallography ◽  
Egg White Lysozyme ◽  
Hen Egg White ◽  
Hen Egg

Metalation of lysozyme with anticancer organometallics results in protein destabilisation, probably relevant in metallodrug mode of action.

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