Targeted proteomics by selected reaction monitoring mass spectrometry: applications to systems biology and biomarker discovery

2011 ◽  
Vol 7 (2) ◽  
pp. 292-303 ◽  
Author(s):  
Sarah Elschenbroich ◽  
Thomas Kislinger
Keyword(s):  
Mass Spectrometry ◽  
Systems Biology ◽  
Biomarker Discovery ◽  
Selected Reaction Monitoring ◽  
Reaction Monitoring ◽  
Targeted Proteomics

scholarly journals High-Throughput Large-Scale Targeted Proteomics Assays for Quantifying Pathway Proteins in Pseudomonas putida KT2440

2020 ◽  
Vol 8 ◽  
Author(s):  
Yuqian Gao ◽  
Thomas L. Fillmore ◽  
Nathalie Munoz ◽  
Gayle J. Bentley ◽  
Christopher W. Johnson ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Systems Biology ◽  
Pseudomonas Putida ◽  
High Throughput ◽  
High Speed ◽  
Large Scale ◽  
Protein Quantification ◽  
Selected Reaction Monitoring ◽  
Targeted Proteomics ◽  
Pseudomonas Putida Kt2440

Targeted proteomics is a mass spectrometry-based protein quantification technique with high sensitivity, accuracy, and reproducibility. As a key component in the multi-omics toolbox of systems biology, targeted liquid chromatography-selected reaction monitoring (LC-SRM) measurements are critical for enzyme and pathway identification and design in metabolic engineering. To fulfill the increasing need for analyzing large sample sets with faster turnaround time in systems biology, high-throughput LC-SRM is greatly needed. Even though nanoflow LC-SRM has better sensitivity, it lacks the speed offered by microflow LC-SRM. Recent advancements in mass spectrometry instrumentation significantly enhance the scan speed and sensitivity of LC-SRM, thereby creating opportunities for applying the high speed of microflow LC-SRM without losing peptide multiplexing power or sacrificing sensitivity. Here, we studied the performance of microflow LC-SRM relative to nanoflow LC-SRM by monitoring 339 peptides representing 132 enzymes in Pseudomonas putida KT2440 grown on various carbon sources. The results from the two LC-SRM platforms are highly correlated. In addition, the response curve study of 248 peptides demonstrates that microflow LC-SRM has comparable sensitivity for the majority of detected peptides and better mass spectrometry signal and chromatography stability than nanoflow LC-SRM.

scholarly journals Selected reaction monitoring mass spectrometry of mastitis milk reveals pathogen-specific regulation of bovine host response proteins

2018 ◽  
Vol 101 (7) ◽  
pp. 6532-6541 ◽  
Author(s):  
Ulrike Kusebauch ◽  
Lorenzo E. Hernández-Castellano ◽  
Stine L. Bislev ◽  
Robert L. Moritz ◽  
Christine M. Røntved ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Host Response ◽  
Selected Reaction Monitoring ◽  
Reaction Monitoring ◽  
Specific Regulation
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