scholarly journals Exploring both sequence detection and restriction endonuclease cleavage kinetics by recognition site via single-molecule microfluidic trapping

Lab on a Chip ◽  
2011 ◽  
Vol 11 (3) ◽  
pp. 435-442 ◽  
Author(s):  
Weilin Xu ◽  
Susan J. Muller
Keyword(s):  
Restriction Endonuclease ◽  
Single Molecule ◽  
Recognition Site ◽  
Sequence Detection ◽  
Restriction Endonuclease Cleavage

scholarly journals Nanopore Technology for the Application of Protein Detection

Nanomaterials ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1942
Author(s):  
Xiaoqing Zeng ◽  
Yang Xiang ◽  
Qianshan Liu ◽  
Liang Wang ◽  
Qianyun Ma ◽  
...  
Keyword(s):  
Single Molecule ◽  
Protein Detection ◽  
High Sensitivity ◽  
Single Molecule Detection ◽  
Sequence Detection ◽  
Fast Detection ◽  
Material Basis ◽  
Sensing Technology ◽  
Detection Technology ◽  
Structure Recognition

Protein is an important component of all the cells and tissues of the human body and is the material basis of life. Its content, sequence, and spatial structure have a great impact on proteomics and human biology. It can reflect the important information of normal or pathophysiological processes and promote the development of new diagnoses and treatment methods. However, the current techniques of proteomics for protein analysis are limited by chemical modifications, large sample sizes, or cumbersome operations. Solving this problem requires overcoming huge challenges. Nanopore single molecule detection technology overcomes this shortcoming. As a new sensing technology, it has the advantages of no labeling, high sensitivity, fast detection speed, real-time monitoring, and simple operation. It is widely used in gene sequencing, detection of peptides and proteins, markers and microorganisms, and other biomolecules and metal ions. Therefore, based on the advantages of novel nanopore single-molecule detection technology, its application to protein sequence detection and structure recognition has also been proposed and developed. In this paper, the application of nanopore single-molecule detection technology in protein detection in recent years is reviewed, and its development prospect is investigated.

Localization of kinetoplast DNA maxicircle transcripts in bloodstream and procyclic form Trypanosoma brucei

1982 ◽  
Vol 2 (7) ◽  
pp. 845-852
Author(s):  
K D Stuart ◽  
S B Gelvin
Keyword(s):  
Restriction Endonuclease ◽  
Trypanosoma Brucei ◽  
Variable Region ◽  
Cleavage Sites ◽  
Kinetoplast Dna ◽  
Coding Sequences ◽  
Procyclic Form ◽  
Restriction Endonuclease Cleavage ◽  
Mitochondrial Respiratory

Over 80% of the maxicircle and numerous minicircles of Trypanosoma brucei kinetoplast DNA have been cloned. The uncloned maxicircle segment contains few restriction endonuclease cleavage sites, varies in size among strains, and may be unstable in conventional cloning systems. cDNA prepared to bloodstream or procyclic trypomastigote RNA hybridized to all but one maxicircle segment, but did not hybridize to minicircles. Fourteen maxicircle transcripts were detected in RNA from both bloodstream and procyclic trypomastigotes. The coding sequences for these transcripts were localized and account for most of the maxicircle. One region of the maxicircle, which borders the variable region, was not found to be transcribed. We conclude that the maxicircle is largely but not completely transcribed in both bloodstream and procyclic trypomastigotes, whereas minicircle transcription is minimal or absent in these stages. Qualitative transcriptional differences which could account for mitochondrial respiratory differences between the bloodstream and procyclic trypomastigotes were not observed.

Restriction endonuclease PshAI from Plesiomonas shigelloides with the novel recognition site 5′-GACNN/NNGTC

Gene ◽  
1990 ◽  
Vol 87 (1) ◽  
pp. 119-122 ◽  
Author(s):  
Michiko Miyahara ◽  
Katsuhisa Nakajima ◽  
Toshio Shimada ◽  
Katsutoshi Mise
Keyword(s):  
Restriction Endonuclease ◽  
Recognition Site ◽  
The Novel ◽  
Plesiomonas Shigelloides
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