Neomycin- and hygromycin-resistance expression cassettes containing an artificial triple-helix site and a synthetic lac operator facilitate restriction endonuclease cleavage at pre-defined sites and recovery of specific fragments from mammalian genomes

1994 ◽  
Vol 5 (3) ◽  
pp. 183-186 ◽  
Author(s):  
M. C. Nehls ◽  
S. Krause ◽  
T. Boehm
Keyword(s):  
Restriction Endonuclease ◽  
Triple Helix ◽  
Hygromycin Resistance ◽  
Lac Operator ◽  
Mammalian Genomes ◽  
Resistance Expression ◽  
Restriction Endonuclease Cleavage

Inhibition of Restriction Endonuclease Cleavage by Triple Helix Formation with RNA and 2‘-O-Methyl RNA Oligonucleotides Containing 8-Oxo-adenosine in Place of Cytidine†

Biochemistry ◽  
1999 ◽  
Vol 38 (20) ◽  
pp. 6570-6575 ◽  
Author(s):  
Kaoru Ushijima ◽  
Toshiaki Ishibashi ◽  
Hidefumi Yamakawa ◽  
Satoru Tsukahara ◽  
Kazuyuki Takai ◽  
...  
Keyword(s):  
Restriction Endonuclease ◽  
Triple Helix ◽  
Helix Formation ◽  
Triple Helix Formation ◽  
Restriction Endonuclease Cleavage

Inhibition of restriction endonuclease cleavage via triple helix formation by homopyrimidine oligonucleotides

Biochemistry ◽  
1989 ◽  
Vol 28 (25) ◽  
pp. 9617-9619 ◽  
Author(s):  
Jean Christophe Francois ◽  
Tula Saison-Behmoaras ◽  
Nguyen Thanh Thuong ◽  
Claude Helene
Keyword(s):  
Restriction Endonuclease ◽  
Triple Helix ◽  
Helix Formation ◽  
Triple Helix Formation ◽  
Restriction Endonuclease Cleavage

Localization of kinetoplast DNA maxicircle transcripts in bloodstream and procyclic form Trypanosoma brucei

1982 ◽  
Vol 2 (7) ◽  
pp. 845-852
Author(s):  
K D Stuart ◽  
S B Gelvin
Keyword(s):  
Restriction Endonuclease ◽  
Trypanosoma Brucei ◽  
Variable Region ◽  
Cleavage Sites ◽  
Kinetoplast Dna ◽  
Coding Sequences ◽  
Procyclic Form ◽  
Restriction Endonuclease Cleavage ◽  
Mitochondrial Respiratory

Over 80% of the maxicircle and numerous minicircles of Trypanosoma brucei kinetoplast DNA have been cloned. The uncloned maxicircle segment contains few restriction endonuclease cleavage sites, varies in size among strains, and may be unstable in conventional cloning systems. cDNA prepared to bloodstream or procyclic trypomastigote RNA hybridized to all but one maxicircle segment, but did not hybridize to minicircles. Fourteen maxicircle transcripts were detected in RNA from both bloodstream and procyclic trypomastigotes. The coding sequences for these transcripts were localized and account for most of the maxicircle. One region of the maxicircle, which borders the variable region, was not found to be transcribed. We conclude that the maxicircle is largely but not completely transcribed in both bloodstream and procyclic trypomastigotes, whereas minicircle transcription is minimal or absent in these stages. Qualitative transcriptional differences which could account for mitochondrial respiratory differences between the bloodstream and procyclic trypomastigotes were not observed.

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