Microfluidic synthesis of Janus particles by UV-directed phase separation

2011 ◽  
Vol 47 (9) ◽  
pp. 2634 ◽  
Author(s):  
Saifullah Lone ◽  
Sung Hoon Kim ◽  
Seong Won Nam ◽  
Sungsu Park ◽  
Jin Joo ◽  
...  
Keyword(s):  
Phase Separation ◽  
Janus Particles

One-step fabrication of polymeric hybrid particles with core–shell, patchy, patchy Janus and Janus architectures via a microfluidic-assisted phase separation process

RSC Advances ◽  
2015 ◽  
Vol 5 (97) ◽  
pp. 79969-79975 ◽  
Author(s):  
Xiaodong Cao ◽  
Wenxiu Li ◽  
Ting Ma ◽  
Hua Dong
Keyword(s):  
Phase Separation ◽  
Separation Process ◽  
Janus Particles ◽  
Core Shell ◽  
Hybrid Particles ◽  
Phase Separation Process ◽  
One Step

We report in this paper a one-step route for the preparation of core–shell, patchy, patchy Janus and Janus particles via a microfluidic-assisted phase separation process.

Facile fabrication of Fe3O4@PS/PGMA magnetic Janus particles via organic–inorganic dual phase separation

RSC Advances ◽  
2014 ◽  
Vol 4 (52) ◽  
pp. 27152 ◽  
Author(s):  
Lei Tian ◽  
Baoliang Zhang ◽  
Wei Li ◽  
Xiangjie Li ◽  
Xinlong Fan ◽  
...  
Keyword(s):  
Phase Separation ◽  
Janus Particles ◽  
Dual Phase ◽  
Facile Fabrication

One-step preparation of magnetic Janus particles using controlled phase separation of polymer blends and nanoparticles

RSC Advances ◽  
2013 ◽  
Vol 3 (29) ◽  
pp. 11801 ◽  
Author(s):  
Joonwoo Jeong ◽  
Eujin Um ◽  
Je-Kyun Park ◽  
Mahn Won Kim
Keyword(s):  
Phase Separation ◽  
Polymer Blends ◽  
Janus Particles ◽  
One Step

scholarly journals Lipid phase separation in vesicles enhances TRAIL-mediated cytotoxicity

2021 ◽  
Author(s):  
Timothy Vu ◽  
Justin A Peruzzi ◽  
Lucas E Sant'Anna ◽  
Neha Kamat
Keyword(s):  
Phase Separation ◽  
Cancer Cells ◽  
Lipid Vesicles ◽  
Critical Parameters ◽  
Janus Particles ◽  
Lipid Phase ◽  
Trail Receptor ◽  
Spatial Presentation ◽  
Lipid Phase Separation ◽  
Control Protein

Ligand spatial presentation and density play important roles in many signaling pathways mediated by cell receptors and are critical parameters when designing protein-conjugated therapeutic nanoparticles. Currently, Janus particles are most often used to spatially control ligand conjugation, but the technological challenge of manufacturing Janus particles limits adoption for translational applications. Here, we demonstrate that lipid phase separation can be used to spatially control protein presentation onto lipid vesicles. We used this system to study the density dependence of TNF-related apoptosis inducing ligand (TRAIL), a model therapeutic protein that exhibits greater cytotoxicity to cancer cells when conjugated onto a vesicle surface than when administered as a soluble protein. Using assays for apoptosis and caspase activity, we show that phase separated TRAIL vesicles induced higher cytotoxicity to Jurkat cancer cells than uniformly-conjugated TRAIL vesicles, and enhanced cytotoxicity was dependent on the TRAIL domain density. We then assessed this relationship in other cancer cell lines and demonstrated that phase separated TRAIL vesicles only enhanced cytotoxicity through one TRAIL receptor, DR5, while another TRAIL receptor, DR4, was unaffected by the TRAIL density. These results indicate unique signaling requirements for each TRAIL receptor and how TRAIL therapy could be tailored depending on the relative levels of expression for cancer receptors of interest. Overall, this work demonstrates a readily adoptable method to control protein conjugation and density on bilayer vesicles that can be easily adopted to other therapeutic nanoparticle systems to improve receptor signaling of nanoparticles targeted to cancer and diseased cells.

scholarly journals Microfluidic Preparation of Janus Microparticles With Temperature and pH Triggered Degradation Properties

2021 ◽  
Vol 9 ◽  
Author(s):  
Zi-Yi Feng ◽  
Tao-Tao Liu ◽  
Zhen-Tao Sang ◽  
Zhen-Sheng Lin ◽  
Xin Su ◽  
...  
Keyword(s):  
Phase Separation ◽  
Drug Release ◽  
Phase Change ◽  
Phase Change Materials ◽  
Janus Particles ◽  
Experimental Conditions ◽  
Application Range ◽  
Physiological Temperature ◽  
Microfluidic Flow ◽  
Degradation Properties

Based on the phase separation phenomenon in micro-droplets, polymer-lipid Janus particles were prepared on a microfluidic flow focusing chip. Phase separation of droplets was caused by solvent volatilization and Janus morphology was formed under the action of interfacial tension. Because phase change from solid to liquid of the lipid hemisphere could be triggered by physiological temperature, the lipid hemisphere could be used for rapid release of drugs. While the polymer we selected was pH sensitive that the polymer hemisphere could degrade under acidic conditions, making it possible to release drugs in a specific pH environment, such as tumor tissues. Janus particles with different structures were obtained by changing the experimental conditions. To widen the application range of the particles, fatty alcohol and fatty acid-based phase change materials were also employed to prepare the particles, such as 1-tetradecanol, 1-hexadecanol and lauric acid. The melting points of these substances are higher than the physiological temperature, which can be applied in fever triggered drug release or in thermotherapy. The introduction of poly (lactic-co-glycolic acid) enabled the formation of multicompartment particles with three distinct materials. With different degradation properties of each compartment, the particles generated in this work may find applications in programmed and sequential drug release triggered by multiple stimuli.

Synthesis of Janus particles via kinetic control of phase separation in emulsion droplets

2013 ◽  
Vol 49 (84) ◽  
pp. 9746 ◽  
Author(s):  
Bing Liu ◽  
Helmuth Möhwald ◽  
Dayang Wang
Keyword(s):  
Phase Separation ◽  
Janus Particles ◽  
Kinetic Control ◽  
Emulsion Droplets

Bulk standards for low-temperature biological x-ray microanalysis

1984 ◽  
Vol 42 ◽  
pp. 282-283
Author(s):  
P. Echlin ◽  
M. McKoon ◽  
E.S. Taylor ◽  
C.E. Thomas ◽  
K.L. Maloney ◽  
...  
Keyword(s):  
Phase Separation ◽  
Low Temperature ◽  
Analytical Method ◽  
Salt Solutions ◽  
Absolute Concentration ◽  
Cooling Process ◽  
X Ray ◽  
The Absolute ◽  
Analytical Procedures ◽  
Electrical Conductors

Although sections of frozen salt solutions have been used as standards for x-ray microanalysis, such solutions are less useful when analysed in the bulk form. They are poor thermal and electrical conductors and severe phase separation occurs during the cooling process. Following a suggestion by Whitecross et al we have made up a series of salt solutions containing a small amount of graphite to improve the sample conductivity. In addition, we have incorporated a polymer to ensure the formation of microcrystalline ice and a consequent homogenity of salt dispersion within the frozen matrix. The mixtures have been used to standardize the analytical procedures applied to frozen hydrated bulk specimens based on the peak/background analytical method and to measure the absolute concentration of elements in developing roots.

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