Lateral flow nucleic acid biosensor for Cu2+ detection in aqueous solution with high sensitivity and selectivity

2010 ◽  
Vol 46 (47) ◽  
pp. 9043 ◽  
Author(s):  
Zhiyuan Fang ◽  
Jing Huang ◽  
Puchang Lie ◽  
Zhuo Xiao ◽  
Chuanyan Ouyang ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Nucleic Acid ◽  
High Sensitivity ◽  
Lateral Flow ◽  
Sensitivity And Selectivity

scholarly journals Nucleic-Acid-Binding Chromophores as Efficient Indicators of Aptamer-Target Interactions

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Kwabena Sarpong ◽  
Bhaskar Datta
Keyword(s):  
Nucleic Acid ◽  
High Sensitivity ◽  
Covalent Modification ◽  
Fluorescence Signal ◽  
Nucleic Acid Binding ◽  
Sensitivity And Selectivity ◽  
Target Molecules ◽  
Diagnostic Applications ◽  
The Stability ◽  
Simple Format

The binding affinity and specificity of nucleic acid aptamers have made them valuable candidates for use as sensors in diagnostic applications. In particular, chromophore-functionalized aptamers offer a relatively simple format for detection and quantification of target molecules. We describe the use of nucleic-acid-staining reagents as an effective tool for detecting and signaling aptamer-target interactions. Aptamers varying in size and structure and targeting a range of molecules have been used in conjunction with commercially available chromophores to indicate and quantify the presence of cognate targets with high sensitivity and selectivity. Our assay precludes the covalent modification of nucleic acids and relies on the differential fluorescence signal of chromophores when complexed with aptamers with or without their cognate target. We also evaluate factors that are critical for the stability of the complex between the aptamer and chromophore in presence or absence of target molecules. Our results indicate the possibility of controlling those factors to enhance the sensitivity of target detection by the aptamers used in such assays.

A lateral flow biosensor for detection of nucleic acids with high sensitivity and selectivity

2012 ◽  
Vol 48 (2) ◽  
pp. 236-238 ◽  
Author(s):  
Puchang Lie ◽  
Jie Liu ◽  
Zhiyuan Fang ◽  
Boying Dun ◽  
Lingwen Zeng
Keyword(s):  
Nucleic Acids ◽  
High Sensitivity ◽  
Lateral Flow ◽  
Sensitivity And Selectivity

Highly Hg2+-sensitive and selective fluorescent sensors in aqueous solution and sensors-encapsulated polymeric membrane

RSC Advances ◽  
2016 ◽  
Vol 6 (13) ◽  
pp. 10401-10411 ◽  
Author(s):  
Sasiwimon Kraithong ◽  
Pattareeya Damrongsak ◽  
Kullatat Suwatpipat ◽  
Jitnapa Sirirak ◽  
Pattanawit Swanglap ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Detection Limits ◽  
High Sensitivity ◽  
Fluorescent Sensors ◽  
Polymeric Membranes ◽  
Polymeric Membrane ◽  
Sensitivity And Selectivity

The sensors in solutions and sensors encapsulated polymeric membranes exhibited high sensitivity and selectivity for Hg2+ detection, with detection limits of 0.2–49 ppb.

scholarly journals Recent Development of Aptamer Sensors for the Quantification of Aflatoxin B1

2019 ◽  
Vol 9 (11) ◽  
pp. 2364 ◽  
Author(s):  
Yongmei Jia ◽  
Guohua Zhou ◽  
Peilian Liu ◽  
Zhiguo Li ◽  
Biao Yu
Keyword(s):  
Nucleic Acid ◽  
Food Quality ◽  
Aflatoxin B1 ◽  
High Selectivity ◽  
High Sensitivity ◽  
Nucleic Acid Sequence ◽  
Recent Developments ◽  
Contaminated Food ◽  
Sensitivity And Selectivity ◽  
Labor Consuming

Aflatoxin B1 (AFB1) is one of the most frequently-found mycotoxins in contaminated food. As the content of mycotoxins is particularly low in food, the development of probes to detect AFB1 in foods with high sensitivity and selectivity is an urgent social need for the evaluation of food quality. Numerous techniques have been developed to monitor AFB1. Nevertheless, most of them require cumbersome, labor-consuming, and sophisticated instruments, which have limited their application. An aptamer is a single, short nucleic acid sequence that is capable of recognizing different targets. Owing to their unique properties, aptamers have been considered as alternatives to antibodies. Aptasensors are considered to be an emerging strategy for the quantification of aflatoxin B1 with high selectivity and sensitivity. In this review, we summarize recent developments in colormetric, electrochemical, SERS, and fluorescent aptasensors for the quantification of AFB1. Finally, the perspectives and current challenges of aptasensors for AFB1 are outlined.

scholarly journals Femtomolar and locus-specific detection of N6-methyladenine in DNA by integrating double-hindered replication and nucleic acid-functionalized MB@Zr-MOF

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Qingyuan Zheng ◽  
Tong Wang ◽  
Xinmin Li ◽  
Husun Qian ◽  
Xintong Bian ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Dna Polymerase ◽  
Dna Analysis ◽  
Electrochemical Biosensor ◽  
High Sensitivity ◽  
High Porosity ◽  
Specific Detection ◽  
Sensitivity And Selectivity ◽  
Negative Surface ◽  
A Site

AbstractIn this study, a novel electrochemical biosensor was constructed for ultrasensitive and locus-specific detection of N6-Methyladenine (m6A) in DNA using double-hindered replication and nucleic acid-coated methylene blue (MB)@Zr-MOF. Based on the combination of m6A-impeded replication and AgI-mediated mismatch replication, this mode could effectively stop the extension of the strand once DNA polymerase encountered m6A site, which specifically distinguish the m6A site from natural A site in DNA. Also, Zr-MOF with high porosity and negative surface potential features was carefully chose to load cationic MB, resulting a stable and robust MB@Zr-MOF electrochemical tag. As a result, the developed biosensor exhibited a wide linear range from 1 fM to 1 nM with detection limit down to 0.89 fM. Profiting from the high sensitivity and selectivity, the biosensing strategy revealed good applicability, which had been demonstrated by quantitating m6A DNA at specific site in biological matrix. Thus, the biosensor provides a promising platform for locus-specific m6A DNA analysis. Graphical Abstract

scholarly journals Comparative Study of Gold and Carbon Nanoparticles in Nucleic Acid Lateral Flow Assay

Nanomaterials ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 741
Author(s):  
Juan Carlos Porras ◽  
Mireia Bernuz ◽  
Jennifer Marfa ◽  
Arnau Pallares-Rusiñol ◽  
Mercè Martí ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Comparative Study ◽  
Carbon Nanoparticles ◽  
Limit Of Detection ◽  
Early Stage ◽  
High Sensitivity ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Complex Samples ◽  
Increased Sensitivity

A lateral flow assay (LFA) is a paper-based, point-of-need test designed to detect a specific analyte in complex samples in low-resource settings. Although LFA has been successfully used in different applications, its use is still limited when high sensitivity is required, especially in the diagnosis of an early-stage condition. The limit of detection (LOD) is clearly related to the signal-generating system used to achieve the visual readout, in many cases involving nanoparticles coupled to a biomolecule, which, when combined, provides sensitivity and specificity, respectively. While colloidal gold is currently the most-used label, other detection systems are being developed. Carbon nanoparticles (CNPs) demonstrate outstanding features to improve the sensitivity of this technology by producing an increased contrast in the paper background. Based on the necessity of sensitivity improvement, the aim of this work is a comparative study, in terms of analytical performance, between commercial streptavidin gold nanoparticles (streptAv-AuNPs) and avidin carbon nanoparticles (Av-CNPs) in a nucleic acid lateral flow assay. The visual LOD of the method was calculated by serial dilution of the DNA template, ranging from 0.0 to 7 pg μL−1/1.5 × 104 CFU mL−1). The LFA achieved visual detection of as low as 2.2 × 10−2 pg μL−1 using Av-CNPs and 8.4 × 10−2 pg μL−1 using streptAv-AuNPs. These LODs could be obtained without the assistance of any instrumentation. The results demonstrate that CNPs showed an increased sensitivity, achieving the nanomolar range even by visual inspection. Furthermore, CNPs are the cheapest labels, and the suspensions are very stable and easy to modify.

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