Magnetic bead-based fluorescence immunoassay for aflatoxin B1 in food using biofunctionalized rhodamine B-doped silica nanoparticles

The Analyst ◽  
2010 ◽  
Vol 135 (10) ◽  
pp. 2661 ◽  
Author(s):  
Dianping Tang ◽  
Yongliang Yu ◽  
Reinhard Niessner ◽  
Manuel Miró ◽  
Dietmar Knopp
Keyword(s):  
Silica Nanoparticles ◽  
Aflatoxin B1 ◽  
Rhodamine B ◽  
Magnetic Bead ◽  
Fluorescence Immunoassay

Multifunctional magnetic bead-based electrochemical immunoassay for the detection of aflatoxin B1 in food

The Analyst ◽  
2009 ◽  
Vol 134 (8) ◽  
pp. 1554 ◽  
Author(s):  
Dianping Tang ◽  
Zhaoyang Zhong ◽  
Reinhard Niessner ◽  
Dietmar Knopp
Keyword(s):  
Aflatoxin B1 ◽  
Magnetic Bead ◽  
Electrochemical Immunoassay

scholarly journals Surface-Induced ARGET ATRP for Silicon Nanoparticles with Fluorescent Polymer Brushes

Polymers ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 1228 ◽  
Author(s):  
Chun-Na Yan ◽  
Lin Xu ◽  
Qing-Di Liu ◽  
Wei Zhang ◽  
Rui Jia ◽  
...  
Keyword(s):  
Silica Nanoparticles ◽  
Hybrid Materials ◽  
Rhodamine B ◽  
Methyl Acrylate ◽  
Polymer Brushes ◽  
Diblock Copolymers ◽  
Polymerization Process ◽  
Good Dispersion ◽  
Fluorescence Characteristics ◽  
Arget Atrp

Well-defined polymer brushes attached to nanoparticles offer an elegant opportunity for surface modification because of their excellent mechanical stability, functional versatility, high graft density as well as controllability of surface properties. This study aimed to prepare hybrid materials with good dispersion in different solvents, and to endow this material with certain fluorescence characteristics. Well-defined diblock copolymers poly (styrene)-b-poly (hydroxyethyl methyl acrylate)–co-poly (hydroxyethyl methyl acrylate- rhodamine B) grafted silica nanoparticles (SNPs-g-PS-b-PHEMA-co-PHEMA-RhB) hybrid materials were synthesized via surface-initiated activators regenerated by electron transfer atom transfer radical polymerization (SI-ARGET ATRP). The SNPs surfaces were modified by 3-aminopropyltriethoxysilane (KH-550) firstly, then the initiators 2-Bromoisobutyryl bromide (BIBB) was attached to SNPs surfaces through the esterification of acyl bromide groups and amidogen groups. The synthetic initiators (SNPs-Br) were further used for the SI-ARGET ATRP of styrene (St), hydroxyethyl methyl acrylate (HEMA) and hydroxyethyl methyl acrylate-rhodamine B (HEMA-RhB). The results indicated that the SI-ARGET ATRP initiator had been immobilized onto SNPs surfaces, the Br atom have located at the end of the main polymer chains, and the polymerization process possessed the characteristic of controlled/“living” polymerization. The SNPs-g-PS-b-PHEMA-co-PHEMA-RhB hybrid materials show good fluorescence performance and good dispersion in water and EtOH but aggregated in THF. This study demonstrates that the SI-ARGET ATRP provided a unique way to tune the polymer brushes structure on silica nanoparticles surface and further broaden the application of SI-ARGET ATRP.

Highly sensitive FRET-based fluorescence immunoassay for aflatoxin B1 using cadmium telluride quantum dots

2013 ◽  
Vol 180 (13-14) ◽  
pp. 1217-1223 ◽  
Author(s):  
Roya Zekavati ◽  
Shahabeddin Safi ◽  
Seyed Jamal Hashemi ◽  
Tavoos Rahmani-Cherati ◽  
Meisam Tabatabaei ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Cadmium Telluride ◽  
Aflatoxin B1 ◽  
Fluorescence Immunoassay ◽  
Highly Sensitive ◽  
Cadmium Telluride Quantum Dots

Fluorescence resonance energy transfer (FRET)-based nanoarchitecture for monitoring deubiquitinating enzyme activity

2020 ◽  
Vol 56 (21) ◽  
pp. 3183-3186 ◽  
Author(s):  
Yan-Yan Liang ◽  
Jie Zhang ◽  
Hui Cui ◽  
Zhen-Shu Shao ◽  
Chen Cheng ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Energy Transfer ◽  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Fluorescence Resonance Energy Transfer ◽  
Rhodamine B ◽  
Mesoporous Silica Nanoparticles ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Deubiquitinating Enzyme

A novel nanoarchitecture (MSN-Tb-UbR) was prepared by modifying rhodamine B-labelled Ubs (Ub-Rs) on the surface of mesoporous silica nanoparticles (MSNs) loaded with Tb3+-complexes.

Optimized Rhodamine B labeled mesoporous silica nanoparticles as fluorescent scaffolds for the immobilization of photosensitizers: a theranostic platform for optical imaging and photodynamic therapy

2016 ◽  
Vol 18 (13) ◽  
pp. 9042-9052 ◽  
Author(s):  
B. Martins Estevão ◽  
I. Miletto ◽  
L. Marchese ◽  
E. Gianotti
Keyword(s):  
Photodynamic Therapy ◽  
Mesoporous Silica ◽  
Contrast Agent ◽  
Optical Imaging ◽  
Silica Nanoparticles ◽  
Rhodamine B ◽  
Mesoporous Silica Nanoparticles

A theranostic nanoplatform coupling optical-imaging with photodynamic-therapy was prepared by using Rhodamine B as a contrast agent and verteporfin as a photosensitizer.

scholarly journals Hybrid inorganic-organic fluorescent silica nanoparticles—influence of dye binding modes on dye leaching

2021 ◽  
Author(s):  
Nadja Klippel ◽  
Gregor Jung ◽  
Guido Kickelbick
Keyword(s):  
Silica Nanoparticles ◽  
Rhodamine B ◽  
Fluorescent Dyes ◽  
Matrix Material ◽  
Fluorescein Isothiocyanate ◽  
Silica Matrix ◽  
Physical Interaction ◽  
Binding Modes ◽  
Xanthene Dyes ◽  
Rhodamine B Isothiocyanate

AbstractSilica nanoparticles with embedded fluorescent dyes represent an important class of markers for example in biological imaging. We systematically studied the various incorporation mechanisms of fluorescent xanthene dyes in 30–40 nm silica nanoparticles. An important parameter was the interaction of the dye with the matrix material, either by weak electrostatic or strong covalent interactions, which also has implications on the stability of fluorescence and brightness of the dyes. Factors that can influence leaching of dyes such as the position of the dyes in particles and the intensity of the particle-dye interaction were investigated by using the solvatochromic effect of xanthene dyes and by stationary fluorescence anisotropy measurements. We compared uranine and rhodamine B, which were physically embedded, with modified fluorescein isothiocyanate and rhodamine B isothiocyanate, which were covalently bound to the silica matrix within a usual Stöber synthesis. Systematic leaching studies of time spans up to 4 days revealed that covalent bonding of dyes like fluorescein isothiocyanate or rhodamine B isothiocyanate is necessary for fluorescence stability, since dyes bound by physical interaction tend to leach out of porous silica networks. Coverage of silica particles with hydrophobic protection layers of alkyltrialkoxysilanes or hydrophilic polyethylene glycol (PEG) groups resulted in a better retention of physisorbed dyes and provides the possibility to adapt the particles to the polarity of the medium. Best results were archived with PEG groups, but even small trimethylsilyl (TMS) groups already reduce leaching.

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