Mediator-free amperometric glucose biosensor based on glucose oxidase entrapped in poly(vinyl alcohol) matrix

The Analyst ◽  
2011 ◽  
Vol 136 (1) ◽  
pp. 164-173 ◽  
Author(s):  
Maria Rachele Guascito ◽  
Daniela Chirizzi ◽  
Cosimino Malitesta ◽  
Elisabetta Mazzotta
Keyword(s):  
Glucose Oxidase ◽  
Vinyl Alcohol ◽  
Glucose Biosensor ◽  
Poly Vinyl Alcohol

Immobilization Of Glucose Oxidase In Freeze – Thawed PVA And PVA/TMOS Membrane

2012 ◽  
Author(s):  
Azila Abdul Aziz ◽  
Norhana Jusoh ◽  
Mohd. Azizul Ashari ◽  
Norhasliza Hassan
Keyword(s):  
Enzyme Activity ◽  
Glucose Oxidase ◽  
Key Words ◽  
Vinyl Alcohol ◽  
Glucose Biosensor ◽  
Poly Vinyl Alcohol ◽  
Immobilization Method

Dalam projek ini, dua kaedah penyekat–gerakan enzim glukosa oksidase (GOD) bagi biosensor glukosa amperometrik berdasarkan peroksida telah dikaji. Bagi kaedah penyekat–gerakan yang pertama, glukosa oksidase telah disekat–gerak dalam polivinilalkohol (PNA) dibeku–cair pada tiga kepekatan PVA yang berbeza (5%, 10% dan 15% PVA). Kepekatan PVA yang lebih tinggi mampu menyekat–gerak enzim di dalam membran dengan lebih baik. Namun, kepekatan PVA yang lebih tinggi tidak semestinya mempunyai korelasi yang baik dengan aktiviti enzim. Membran PVA–GOD dibeku–cair dengan 10% PVA menunjukkan sensitiviti yang paling baik kepada glukosa. Prestasi membran PVA–GOD dibeku–cair dengan 15% PVA dan 5% PVA adalah hampir sama. Bagi kaedah penyekat–gerakan yang kedua, GOD disekat–gerak dalam PVA tetrametoksisilan (TMOS) pada kepekatan (3–glisidoksipropildimetiletoksi) silan yang berbeza. Bagi membran yang disediakan menggunakan 1:1 (TMOS: 3GPDES), peratusan enzim aktiviti yang tinggal selepas hari ke 40 ialah lebih kurang 51%. Membran yang disediakan menggunakan 1:2 (TMOS: 3GPDES) dan 1:3 (TMOS:3GPDES) adalah agak stabil dengan masing–masing sebanyak 69% dan 58% peratusan enzim aktiviti yang tinggal selepas hari ke 40. Keseluruhannya, biosensor glukosa telah menunjukkan prestasi yang memberangsangkan. Kata kunci: Biosensor; glukosa; peroksida; kepekatan In this work, two methods of glucose oxidase (GOD) immobilization for peroxide based glucose biosensor had been investigated. For the first immobilization method, GOD was immobilized in freeze–thawed poly (vinyl alcohol) (PVA) at three different PVA concentrations (5%, 10% and 15% PVA). The higher the PVA concentration used for immobilization, the better the retention of the enzyme in the membrane. Nevertheless, higher PVA concentration did not necessarily correlate well with enzyme activity. Freeze–thawed PVA–GOD membranes with 10% PVA showed the highest sensitivity to glucose. The performances of freeze–thawed PVA–GOD membranes with 15% and 5% PVA was comparable. For the second immobilization method, GOD was immobilized within PVA/tetramethoxysilane (TMOS) matrix with different (3–glycidoxypropyldimenthylethoxy) silane (3GPDES) concentrations. For the membranes prepared with 1:1 (TMOS: 3GPDES) volumetric ratio, the percentage of enzyme activity remaining at day 40 was about 51%. Meanwhile, membrane prepared with 1:2 (TMOS: 3GPDES) and 1:3 (TMOS: 3GPDES) were quite stable with 69% and 58% of enzyme activity remaining at day 40, respectively. Overall, the glucose biosensor showed very promising performances. Key words: Biosensor; peroxide; temperature; concentration

Immobilization of invertase and glucose oxidase in conducting copolymers of thiophene functionalized poly(vinyl alcohol) with pyrrole

2006 ◽  
Vol 66 (3) ◽  
pp. 365-371 ◽  
Author(s):  
Ertugrul Sahmetlioglu ◽  
Hüseyin Yürük ◽  
Levent Toppare ◽  
Ioan Cianga ◽  
Yusuf Yagci
Keyword(s):  
Glucose Oxidase ◽  
Vinyl Alcohol ◽  
Poly Vinyl Alcohol ◽  
Conducting Copolymers

Glucose-induced decomposition of layer-by-layer films composed of phenylboronic acid-bearing poly(allylamine) and poly(vinyl alcohol) under physiological conditions

2015 ◽  
Vol 3 (39) ◽  
pp. 7796-7802 ◽  
Author(s):  
Katsuhiko Sato ◽  
Mao Takahashi ◽  
Megumi Ito ◽  
Eiichi Abe ◽  
Jun-Ichi Anzai
Keyword(s):  
Glucose Oxidase ◽  
Vinyl Alcohol ◽  
Phenylboronic Acid ◽  
Poly Vinyl Alcohol ◽  
Layer By Layer ◽  
Physiological Conditions ◽  
Induced Decomposition

Phenylboronic acid-bearing poly(allylamine)/poly(vinyl alcohol) layer-by-layer films coupled with glucose oxidase decomposed in the presence of glucose under physiological conditions.

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