Immobilization of trypsin on water-soluble dendrimer-modified carbon nanotubes for on-plate proteolysis combined with MALDI-MS analysis

2010 ◽  
Vol 6 (8) ◽  
pp. 1447 ◽  
Author(s):  
Ying Zhang ◽  
Weiman Cao ◽  
Minbo Liu ◽  
Shiping Yang ◽  
Huixia Wu ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Water Soluble ◽  
Maldi Ms ◽  
Ms Analysis ◽  
Modified Carbon Nanotubes

scholarly journals Carbon nanotubes as affinity probes for peptides and proteins in MALDI MS analysis

2004 ◽  
Vol 15 (11) ◽  
pp. 1629-1635 ◽  
Author(s):  
Wei-Yu Chen ◽  
Lung-Shen Wang ◽  
Hsin-Tien Chiu ◽  
Yu-Chie Chen ◽  
Chi-Young Lee
Keyword(s):  
Carbon Nanotubes ◽  
Maldi Ms ◽  
Ms Analysis ◽  
Affinity Probes

scholarly journals Water-Soluble Pyrene Tags Enable the Detection of Carbohydrates by Label-Assisted Laser Desorption/ionisation Mass Spectrometry

2020 ◽  
Author(s):  
Jacob R Hauser ◽  
Edmund T Bergström ◽  
Alexander N. Kulak ◽  
Stuart L Warriner ◽  
Jane E Thomas-Oates ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Biological Samples ◽  
Laser Desorption ◽  
Water Soluble ◽  
Maldi Ms ◽  
Ms Analysis ◽  
Ionisation Mass Spectrometry ◽  
Ionisation Mass ◽  
Reducing Carbohydrates

<div>Matrix-assisted laser desorption/ionisation mass spectrometry (MALDI-MS) is widely used for the analysis of biomolecules. Label-assisted laser desorption/ionisation mass spectrometry (LALDI-MS) is a matrix-free variant of MALDI-MS, in which only analytes covalently attached to a laser desorption/ionisation (LDI) enhancer are detected. LALDI-MS has shown promise in overcoming limitations of MALDI-MS in terms of sample preparation and MS analysis. In this work, we have developed water-soluble pyrene-based LDI reagents (LALDI tags) that can be used for (in situ) labelling and LALDI-MS analysis of reducing carbohydrates from complex (biological) samples without the need for additional chemical derivatisation or purification. We have systematically explored the suitability of four pyrene-based LDI enhancers and three aldehyde-reactive handles, optimised sample preparation, and exemplified the use of a LALDI tag by the detection of lactose in cow’s milk. These results demonstrate that LALDI-MS is a promising technique for the analysis of reducing carbohydrates in biological samples, and pave the way for the development of LALDI-MS for glycomics and diagnostics.</div>

scholarly journals Water-Soluble Pyrene Tags Enable the Detection of Carbohydrates by Label-Assisted Laser Desorption/ionisation Mass Spectrometry

2020 ◽  
Author(s):  
Jacob R Hauser ◽  
Edmund T Bergström ◽  
Alexander N. Kulak ◽  
Stuart L Warriner ◽  
Jane E Thomas-Oates ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Biological Samples ◽  
Laser Desorption ◽  
Water Soluble ◽  
Maldi Ms ◽  
Ms Analysis ◽  
Ionisation Mass Spectrometry ◽  
Ionisation Mass ◽  
Reducing Carbohydrates

<div>Matrix-assisted laser desorption/ionisation mass spectrometry (MALDI-MS) is widely used for the analysis of biomolecules. Label-assisted laser desorption/ionisation mass spectrometry (LALDI-MS) is a matrix-free variant of MALDI-MS, in which only analytes covalently attached to a laser desorption/ionisation (LDI) enhancer are detected. LALDI-MS has shown promise in overcoming limitations of MALDI-MS in terms of sample preparation and MS analysis. In this work, we have developed water-soluble pyrene-based LDI reagents (LALDI tags) that can be used for (in situ) labelling and LALDI-MS analysis of reducing carbohydrates from complex (biological) samples without the need for additional chemical derivatisation or purification. We have systematically explored the suitability of four pyrene-based LDI enhancers and three aldehyde-reactive handles, optimised sample preparation, and exemplified the use of a LALDI tag by the detection of lactose in cow’s milk. These results demonstrate that LALDI-MS is a promising technique for the analysis of reducing carbohydrates in biological samples, and pave the way for the development of LALDI-MS for glycomics and diagnostics.</div>

scholarly journals Water-Soluble Pyrene Tags Enable the Detection of Carbohydrates by Label-Assisted Laser Desorption/ionisation Mass Spectrometry

2020 ◽  
Author(s):  
Jacob R Hauser ◽  
Edmund T Bergström ◽  
Alexander N. Kulak ◽  
Stuart L Warriner ◽  
Jane E Thomas-Oates ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Biological Samples ◽  
Laser Desorption ◽  
Water Soluble ◽  
Maldi Ms ◽  
Ms Analysis ◽  
Ionisation Mass Spectrometry ◽  
Ionisation Mass ◽  
Reducing Carbohydrates

<div>Matrix-assisted laser desorption/ionisation mass spectrometry (MALDI-MS) is widely used for the</div><div>analysis of biomolecules. Label-assisted laser desorption/ionisation mass spectrometry (LALDI-MS) is</div><div>a matrix-free variant of MALDI-MS, in which only analytes covalently attached to a laser</div><div>desorption/ionisation (LDI) enhancer are detected. LALDI-MS has shown promise in overcoming</div><div>limitations of MALDI-MS in terms of sample preparation and MS analysis. In this work, we have</div><div>developed water-soluble pyrene-based LDI reagents (LALDI tags) that can be used for (in situ) labelling</div><div>and LALDI-MS analysis of reducing carbohydrates from complex (biological) samples without the need</div><div>for additional chemical derivatisation or purification. We have systematically explored the suitability</div><div>of four pyrene-based LDI enhancers and three aldehyde-reactive handles, optimised sample</div><div>preparation, and exemplified the use of a LALDI tag by the detection of lactose in cow’s milk. These</div><div>results demonstrate that LALDI-MS is a promising technique for the analysis of reducing carbohydrates</div><div>in biological samples, and pave the way for the development of LALDI-MS for glycomics and</div><div>diagnostics.</div>

Molecularly Imprinted Sensor for Ascorbic Acid Based on Gold Nanoparticles and Multiwalled Carbon Nanotubes

2020 ◽  
Vol 16 (7) ◽  
pp. 905-913
Author(s):  
Youyuan Peng ◽  
Qingshan Miao
Keyword(s):  
Carbon Nanotubes ◽  
Gold Nanoparticles ◽  
Ascorbic Acid ◽  
Multiwalled Carbon Nanotubes ◽  
Ph Value ◽  
Biological Fluids ◽  
Water Soluble ◽  
Multiwalled Carbon ◽  
Experimental Conditions ◽  
Molecularly Imprinted

Background: L-Ascorbic acid (AA) is a kind of water soluble vitamin, which is mainly present in fruits, vegetables and biological fluids. As a low cost antioxidant and effective scavenger of free radicals, AA may help to prevent diseases such as cancer and Parkinson’s disease. Owing to its role in the biological metabolism, AA has also been utilized for the therapy of mental illness, common cold and for improving the immunity. Therefore, it is very necessary and urgent to develop a simple, rapid and selective strategy for the detection of AA in various samples. Methods: The molecularly imprinted poly(o-phenylenediamine) (PoPD) film was prepared for the analysis of L-ascorbic acid (AA) on gold nanoparticles (AuNPs) - multiwalled carbon nanotubes (MWCNTs) modified glass carbon electrode (GCE) by electropolymerization of o-phenylenediamine (oPD) and AA. Experimental parameters including pH value of running buffer and scan rates were optimized. Scanning electron microscope (SEM), fourier-transform infrared (FTIR) spectra, cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were utilized for the characterization of the imprinted polymer film. Results: Under the selected experimental conditions, the DPV peak currents of AA exhibit two distinct linear responses ranging from 0.01 to 2 μmol L-1 and 2 to 100 μmol L-1 towards the concentrations of AA, and the detection limit was 2 nmol L-1 (S/N=3). Conclusion: The proposed electrochemical sensor possesses excellent selectivity for AA, along with good reproducibility and stability. The results obtained from the analysis of AA in real samples demonstrated the applicability of the proposed sensor to practical analysis.

Sign in / Sign up

Export Citation Format

Share Document