Glucosamine hydrochloride functionalized tetraphenylethylene: A novel fluorescent probe for alkaline phosphatase based on the aggregation-induced emission

2010 ◽  
Vol 46 (23) ◽  
pp. 4067 ◽  
Author(s):  
Qi Chen ◽  
Ning Bian ◽  
Chun Cao ◽  
Xi-Long Qiu ◽  
Ai-Di Qi ◽  
...  
RSC Advances ◽  
2018 ◽  
Vol 8 (27) ◽  
pp. 14995-15000 ◽  
Author(s):  
Wenjuan Zhang ◽  
Hanxiao Yang ◽  
Nan Li ◽  
Na Zhao

A sensitive fluorescent probe (TPEQN-P) was designed and synthesized for detecting alkaline phosphatase and monitoring its enzymatic activity based on the specific aggregation-induced emission effect.


Talanta ◽  
2019 ◽  
Vol 205 ◽  
pp. 120143 ◽  
Author(s):  
Yaqian Li ◽  
Ruihua Xie ◽  
Xiao Pang ◽  
Zile Zhou ◽  
Hai Xu ◽  
...  

Talanta ◽  
2018 ◽  
Vol 178 ◽  
pp. 847-853 ◽  
Author(s):  
Chenchen Liu ◽  
Yandi Hang ◽  
Tao Jiang ◽  
Ji Yang ◽  
Xiao Zhang ◽  
...  

2013 ◽  
Vol 96 (2) ◽  
pp. 495-499 ◽  
Author(s):  
De-Xun Xie ◽  
Zhao-Jin Ran ◽  
Zhen Jin ◽  
Xiao-Bing Zhang ◽  
De-Lie An

Luminescence ◽  
2021 ◽  
Author(s):  
Longqi Xue ◽  
Liangqiang Wu ◽  
Yapeng Li ◽  
Qingbiao Yang ◽  
Dazhi Sun ◽  
...  

1992 ◽  
Vol 263 (3) ◽  
pp. G371-G379
Author(s):  
B. L. Black ◽  
J. O. Rogers

The fluorescent probe fura-2 was used to assay Ca2+ levels in epithelial cell suspensions from embryonic and neonatal chick duodenum. Cell preparations maintained high viability, completely hydrolyzed fura-2/AM to fura-2, retained 92% of cellular fura-2 within the cytoplasmic compartment, and gave low autofluorescence values during assay. Fura-2 leakage from loaded cells occurred at all ages, but could be corrected for in subsequent calculations of cellular Ca2+. Cytoplasmic Ca2+ concentration was 76-80 nM in cells from 14- to 16-day embryonic intestine, rose significantly to 92-98 nM at 17-20 days, and reached 209 nM at 1-day post-hatch when assayed in buffers containing 1.3 mM Ca2+. The developmental rise in cytoplasmic Ca2+ was accompanied by an enhanced ability of cells to maintain a constant Ca2+ concentration at increased levels of extracellular Ca2+ and by a highly correlated rise in alkaline phosphatase (ALP) activity. Epithelial Ca2+ subsequently decreased to the "adult" value of 133-142 nM and was constant along the crypt-villus axis of neonatal intestine. These results verify that fura-2 can be used to compare baseline cytoplasmic Ca2+ values of epithelial cells from developing intestine, reveal that significant changes in Ca2+ homeostasis occur during ontogeny, and suggest that epithelial Ca2+ may modulate ALP activity during the differentiation of embryonic enterocytes.


ACS Sensors ◽  
2018 ◽  
Vol 3 (10) ◽  
pp. 2112-2117 ◽  
Author(s):  
Xujie Zhao ◽  
Chendong Ji ◽  
Le Ma ◽  
Zhen Wu ◽  
Wenyu Cheng ◽  
...  

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