Red–Near-Infrared Fluorescent Probe for Time-Resolved in Vivo Alkaline Phosphatase Detection with the Assistance of a Photoresponsive Nanocontainer

2019 ◽  
Vol 91 (20) ◽  
pp. 13174-13182 ◽  
Author(s):  
Xu Jie ◽  
Mei Wu ◽  
Haimei Yang ◽  
Weili Wei
Keyword(s):  
Alkaline Phosphatase ◽  
Fluorescent Probe ◽  
Near Infrared ◽  
Time Resolved

A novel near-infrared fluorescent probe for detecting intracellular alkaline phosphatase and imaging of living cells

2019 ◽  
Vol 7 (8) ◽  
pp. 1284-1291 ◽  
Author(s):  
Longbin Xu ◽  
Xu He ◽  
Yibing Huang ◽  
Pinyi Ma ◽  
Yanxiao Jiang ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Fluorescent Probe ◽  
Near Infrared ◽  
Living Cells ◽  
Design Synthesis

Design, synthesis and application of a fluorescent probe with a novel near-infrared fluorophore for in vivo imaging of alkaline phosphatase.

In vivo imaging of alkaline phosphatase in tumor-bearing mouse model by a promising near-infrared fluorescent probe

Talanta ◽  
2017 ◽  
Vol 175 ◽  
pp. 421-426 ◽  
Author(s):  
Hong-Wen Liu ◽  
Xiao-Xiao Hu ◽  
Longmin Zhu ◽  
Ke Li ◽  
Qiming Rong ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Mouse Model ◽  
Fluorescent Probe ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Tumor Bearing Mouse ◽  
Tumor Bearing

scholarly journals Real-time observation of tetrapyrrole binding to an engineered bacterial phytochrome

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Yusaku Hontani ◽  
Mikhail Baloban ◽  
Francisco Velazquez Escobar ◽  
Swetta A. Jansen ◽  
Daria M. Shcherbakova ◽  
...  
Keyword(s):  
Real Time ◽  
Rational Design ◽  
Near Infrared ◽  
Fluorescent Proteins ◽  
Covalent Bond ◽  
Binding Pocket ◽  
Tissue Imaging ◽  
Covalent Attachment ◽  
Time Resolved

AbstractNear-infrared fluorescent proteins (NIR FPs) engineered from bacterial phytochromes are widely used for structural and functional deep-tissue imaging in vivo. To fluoresce, NIR FPs covalently bind a chromophore, such as biliverdin IXa tetrapyrrole. The efficiency of biliverdin binding directly affects the fluorescence properties, rendering understanding of its molecular mechanism of major importance. miRFP proteins constitute a family of bright monomeric NIR FPs that comprise a Per-ARNT-Sim (PAS) and cGMP-specific phosphodiesterases - Adenylyl cyclases - FhlA (GAF) domain. Here, we structurally analyze biliverdin binding to miRFPs in real time using time-resolved stimulated Raman spectroscopy and quantum mechanics/molecular mechanics (QM/MM) calculations. Biliverdin undergoes isomerization, localization to its binding pocket, and pyrrolenine nitrogen protonation in <1 min, followed by hydrogen bond rearrangement in ~2 min. The covalent attachment to a cysteine in the GAF domain was detected in 4.3 min and 19 min in miRFP670 and its C20A mutant, respectively. In miRFP670, a second C–S covalent bond formation to a cysteine in the PAS domain occurred in 14 min, providing a rigid tetrapyrrole structure with high brightness. Our findings provide insights for the rational design of NIR FPs and a novel method to assess cofactor binding to light-sensitive proteins.

scholarly journals A Versatile Setup for Time-Resolved Functional Near Infrared Spectroscopy Based on Fast-Gated Single-Photon Avalanche Diode and on Four-Wave Mixing Laser

2019 ◽  
Vol 9 (11) ◽  
pp. 2366 ◽  
Author(s):  
Laura Di Sieno ◽  
Alberto Dalla Mora ◽  
Alessandro Torricelli ◽  
Lorenzo Spinelli ◽  
Rebecca Re ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Near Infrared Spectroscopy ◽  
Near Infrared ◽  
Single Photon ◽  
Wave Mixing ◽  
Functional Near Infrared Spectroscopy ◽  
Time Resolved ◽  
Diffusive Medium ◽  
Spectroscopy System

In this paper, a time-domain fast gated near-infrared spectroscopy system is presented. The system is composed of a fiber-based laser providing two pulsed sources and two fast gated detectors. The system is characterized on phantoms and was tested in vivo, showing how the gating approach can improve the contrast and contrast-to-noise-ratio for detection of absorption perturbation inside a diffusive medium, regardless of source-detector separation.

A novel colorimetric and near-infrared fluorescent probe for hydrogen peroxide imaging in vitro and in vivo

RSC Advances ◽  
2015 ◽  
Vol 5 (104) ◽  
pp. 85957-85963 ◽  
Author(s):  
Peng Wang ◽  
Ke Wang ◽  
Dan Chen ◽  
Yibo Mao ◽  
Yueqing Gu
Keyword(s):  
Hydrogen Peroxide ◽  
Fluorescent Probe ◽  
Near Infrared

A novel NIR fluorescent probe (DCM-B2) based on dicyanomethylene-4H-pyran was synthesized for the detection of H2O2.

A near-infrared reversible fluorescent probe for real-time imaging of redox status changes in vivo

2013 ◽  
Vol 4 (3) ◽  
pp. 1079 ◽  
Author(s):  
Kehua Xu ◽  
Mingming Qiang ◽  
Wen Gao ◽  
Ruixian Su ◽  
Na Li ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescent Probe ◽  
Near Infrared ◽  
Redox Status ◽  
Real Time Imaging

scholarly journals <italic>In vivo</italic> imaging of penicillin G acylase in mice via a near-infrared fluorescent probe

2017 ◽  
Vol 47 (8) ◽  
pp. 994-999
Author(s):  
Xiaohua Li ◽  
Xinyuan He ◽  
Wen Shi ◽  
Haolin Zhang ◽  
Huimin Ma
Keyword(s):  
Fluorescent Probe ◽  
Near Infrared ◽  
Penicillin G Acylase ◽  
Penicillin G
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