Hairpin DNA coated gold nanoparticles as intracellular mRNA probes for the detection of tyrosinase gene expression in melanoma cells

2010 ◽  
Vol 46 (30) ◽  
pp. 5557 ◽  
Author(s):  
S. Reese Harry ◽  
Donna J. Hicks ◽  
Katayoun I. Amiri ◽  
David W. Wright
Keyword(s):  
Gene Expression ◽  
Gold Nanoparticles ◽  
Melanoma Cells ◽  
Hairpin Dna ◽  
Tyrosinase Gene

Sulforaphane Inhibited Melanin Synthesis by Regulating Tyrosinase Gene Expression in B16 Mouse Melanoma Cells

2010 ◽  
Vol 74 (3) ◽  
pp. 579-582 ◽  
Author(s):  
Ichiro SHIRASUGI ◽  
Miyuki KAMADA ◽  
Takashi MATSUI ◽  
Yoichi SAKAKIBARA ◽  
Ming-Cheh LIU ◽  
...  
Keyword(s):  
Gene Expression ◽  
Melanoma Cells ◽  
Melanin Synthesis ◽  
Mouse Melanoma ◽  
Tyrosinase Gene ◽  
B16 Mouse Melanoma

scholarly journals Regulation of tyrosinase gene expression by cAMP in B16 melanoma cells involves two CATGTG motifs surrounding the TATA box: implication of the microphthalmia gene product.

1996 ◽  
Vol 134 (3) ◽  
pp. 747-755 ◽  
Author(s):  
C Bertolotto ◽  
K Bille ◽  
J P Ortonne ◽  
R Ballotti
Keyword(s):  
Gene Expression ◽  
Transcriptional Activity ◽  
Transcriptional Start Site ◽  
Regulatory Elements ◽  
Melanoma Cells ◽  
Tata Box ◽  
Luciferase Reporter ◽  
Tyrosinase Gene ◽  
Luciferase Reporter Plasmid ◽  
E Box

In melanocytes and in melanoma cells, upregulation of melanogenesis, by cAMP elevating agents, results from a stimulation of tyrosinase activity that has been ascribed to an increase in tyrosinase protein and messenger amount. However, the mechanism by which cAMP elevating agents increase tyrosinase mRNA remains to be elucidated. In this study, using a luciferase reporter plasmid containing the 2.2-kb fragment 5' of the transcriptional start site of the mouse tyrosinase gene, we showed that cAMP elevating agents lead to a strong stimulation (20-fold) of transcriptional activity of the tyrosinase promoter. Deletions and mutations in the mouse tyrosinase promoter showed that the M-box 70-bp upstream from the TATA-box and the E-box located downstream the TATA-box, near to the initiator site, are involved in the regulation of the tyrosinase promoter activity by cAMP. Additionally, we showed that microphthalmia, a b-HLH transcription factor associated with pigmentation disorders in mouse, binds to these regulatory elements and modulates the transcriptional activity of the tyrosinase promoter. Since cAMP stimulates the binding of microphthalmia to the M-box and to the E-box; it is tempting to propose that microphthalmia, through its interaction with cis-acting elements surrounding the TATA-box, plays a key role in the regulation of the mouse tyrosinase gene expression by cAMP.

Glucocorticoid Stimulates Melanogenesis and Tyrosinase Gene Expression in B16 Melanoma Cells

1991 ◽  
Vol 4 (5-6) ◽  
pp. 247-251 ◽  
Author(s):  
AKIRA ITO ◽  
CHIKAKO TANAKA ◽  
TAKUJI TAKEUCHI ◽  
YUTAKA MISHIMAI
Keyword(s):  
Gene Expression ◽  
Melanoma Cells ◽  
B16 Melanoma ◽  
B16 Melanoma Cells ◽  
Tyrosinase Gene

scholarly journals Evaluation of melanogenesis in A-375 melanoma cells treated with 5,7-dimethoxycoumarin and valproic acid

2012 ◽  
Vol 17 (4) ◽  
Author(s):  
Ewa Chodurek ◽  
Arkadiusz Orchel ◽  
Joanna Orchel ◽  
Sławomir Kurkiewicz ◽  
Natalia Gawlik ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mass Spectrometry ◽  
Valproic Acid ◽  
Histone Deacetylase Inhibitors ◽  
Degradation Products ◽  
Melanoma Cells ◽  
Synthetic Compounds ◽  
Tyrosinase Gene ◽  
New Treatment ◽  
Melanoma Malignum

AbstractMalignant melanoma (melanoma malignum) is one of the most dangerous types of tumor. It is very difficult to cure. In recent years, a lot of attention has been given to chemoprevention. This method uses natural and synthetic compounds to interfere with and inhibit the process of carcinogenesis. In this study, a new treatment strategy was proposed consisting of a combination of 5,7-dimethoxycoumarin (DMC), an activator of melanogenesis, and valproic acid (VPA), a well-known drug that is one of the histone deacetylase inhibitors (HDACis). In conjunction with 1 mM VPA, all of the tested concentrations of DMC (10–150 μM) significantly decreased the proliferation of A-375 cells. VPA and DMC also induced the synthesis of melanin and the formation of dendrite and star-shaped cells. Tyrosinase gene expression and tyrosinase activity significantly increased in response to VPA treatment. Pyrolysis with gas chromatography and mass spectrometry (Py-GC/MS) was used to investigate the structure of the isolated melanin. This showed that the quantitative and qualitative components of melanin degradation products are dependent on the type of applied melanogenesis inductor. Products derived from eumelanin were detected in the pyrolytic profile of melanin isolated from A-375 cells stimulated with DMC. Thermal degradation of melanin isolated from melanoma cells after exposure to VPA or a mixture of VPA and DMC revealed the additional presence of products derived from pheomelanin.

Ficus deltoidea (Mas cotek) extract exerted anti-melanogenic activity by preventing tyrosinase activity in vitro and by suppressing tyrosinase gene expression in B16F1 melanoma cells

2010 ◽  
Vol 303 (3) ◽  
pp. 161-170 ◽  
Author(s):  
Myoung-Jin Oh ◽  
Mariani Abdul Hamid ◽  
Sulaiman Ngadiran ◽  
Young-Kwon Seo ◽  
Mohamad Roji Sarmidi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Melanoma Cells ◽  
Tyrosinase Activity ◽  
Ficus Deltoidea ◽  
Tyrosinase Gene

Comparative gene expression study in WM164 melanoma cells treated with dimethylacrylshikonin.

2017 ◽  
Author(s):  
N Kretschmer ◽  
A Deutsch ◽  
B Rinner ◽  
M Scheideler ◽  
R Bauer
Keyword(s):  
Gene Expression ◽  
Melanoma Cells ◽  
Gene Expression Study ◽  
Expression Study
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