Perdeuterated pyridinium molten salt (ionic liquid) for direct dissolution and NMR analysis of plant cell walls

2009 ◽  
Vol 11 (11) ◽  
pp. 1762 ◽  
Author(s):  
Nan Jiang ◽  
Yunqiao Pu ◽  
Reichel Samuel ◽  
Arthur J. Ragauskas
Keyword(s):  
Ionic Liquid ◽  
Molten Salt ◽  
Plant Cell ◽  
Cell Walls ◽  
Plant Cell Walls ◽  
Nmr Analysis ◽  
Direct Dissolution

Dissolution and acetylation of ball-milled lignocellulosic biomass in ionic liquids at room temperature: application to nuclear magnetic resonance analysis of cell-wall components

Holzforschung ◽  
2013 ◽  
Vol 67 (1) ◽  
pp. 25-32 ◽  
Author(s):  
Chen Qu ◽  
Takao Kishimoto ◽  
Masahiro Hamada ◽  
Noriyuki Nakajima
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Lignocellulosic Biomass ◽  
Plant Cell ◽  
Cell Walls ◽  
Nuclear Magnetic Resonance Analysis ◽  
Plant Cell Walls ◽  
Nmr Analysis ◽  
Entire Plant ◽  
Nuclear Magnetic

Abstract Nuclear magnetic resonance (NMR) analysis of entire plant cell walls without isolating their components gained importance over the last decade. Recently, a dissolution method and an NMR analysis of entire plant cell walls were reported, in which finely ball-milled lignocellulosic biomass was dissolved at 100°C in the ionic liquid (IL) 1-butyl-3-methylimidazolium chloride. In the present study, several ILs were examined with or without co-solvent to find milder dissolution conditions to mitigate possible degradation processes. Co-solvents, including N,N-dimethylacetamide, pyridine, and dimethyl sulfoxide, increased the solubilization efficiency of ILs. For example, ball-milled fir wood was completely dissolved in 1-allyl-3-methylimidazolium chloride ([Amim]Cl) at 30°C in the presence of these co-solvents. The heteronuclear single-quantum coherence NMR spectra of acetylated fir, birch, and bamboo cell walls, which were recovered from [Amim]Cl/pyridine (1:1, w/w) solution, had a high analytical power.

Exploration of cell wall architecture with the rapid-freeze deep-etch technique

1993 ◽  
Vol 51 ◽  
pp. 128-129
Author(s):  
Béatrice Satiat-Jeunemaitre ◽  
Chris Hawes
Keyword(s):  
Plant Cell ◽  
Cell Walls ◽  
Cell Biology ◽  
Molecular Model ◽  
Three Dimensional ◽  
Secretory Activity ◽  
Wall Structure ◽  
Specimen Preparation ◽  
Molecular Architecture ◽  
Plant Cell Walls

The comprehension of the molecular architecture of plant cell walls is one of the best examples in cell biology which illustrates how developments in microscopy have extended the frontiers of a topic. Indeed from the first electron microscope observation of cell walls it has become apparent that our understanding of wall structure has advanced hand in hand with improvements in the technology of specimen preparation for electron microscopy. Cell walls are sub-cellular compartments outside the peripheral plasma membrane, the construction of which depends on a complex cellular biosynthetic and secretory activity (1). They are composed of interwoven polymers, synthesised independently, which together perform a number of varied functions. Biochemical studies have provided us with much data on the varied molecular composition of plant cell walls. However, the detailed intermolecular relationships and the three dimensional arrangement of the polymers in situ remains a mystery. The difficulty in establishing a general molecular model for plant cell walls is also complicated by the vast diversity in wall composition among plant species.

Wheat cell walls and constituent polysaccharides induce similar microbiota profiles upon in vitro fermentation despite different short chain fatty acid end-product levels.

2021 ◽  
Author(s):  
Shiyi Lu ◽  
Deirdre Mikkelsen ◽  
Hong Yao ◽  
Barbara Williams ◽  
Bernadine Flanagan ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Gut Microbiota ◽  
Plant Cell ◽  
Cell Walls ◽  
Short Chain Fatty Acid ◽  
Chain Fatty Acid ◽  
Plant Cell Walls ◽  
Human Gut ◽  
In Vitro Fermentation

Plant cell walls as well as their component polysaccharides in foods can be utilized to alter and maintain a beneficial human gut microbiota, but it is not known whether the...

scholarly journals Plant Cell Wall Hydration and Plant Physiology: An Exploration of the Consequences of Direct Effects of Water Deficit on the Plant Cell Wall

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1263
Author(s):  
David Stuart Thompson ◽  
Azharul Islam
Keyword(s):  
Cell Wall ◽  
Water Content ◽  
Bacterial Cellulose ◽  
Plant Cell ◽  
Cell Walls ◽  
Plant Cell Wall ◽  
Plant Cell Walls ◽  
Cell Wall Polysaccharides ◽  
Degree Of Hydration ◽  
Cellulose Composites

The extensibility of synthetic polymers is routinely modulated by the addition of lower molecular weight spacing molecules known as plasticizers, and there is some evidence that water may have similar effects on plant cell walls. Furthermore, it appears that changes in wall hydration could affect wall behavior to a degree that seems likely to have physiological consequences at water potentials that many plants would experience under field conditions. Osmotica large enough to be excluded from plant cell walls and bacterial cellulose composites with other cell wall polysaccharides were used to alter their water content and to demonstrate that the relationship between water potential and degree of hydration of these materials is affected by their composition. Additionally, it was found that expansins facilitate rehydration of bacterial cellulose and cellulose composites and cause swelling of plant cell wall fragments in suspension and that these responses are also affected by polysaccharide composition. Given these observations, it seems probable that plant environmental responses include measures to regulate cell wall water content or mitigate the consequences of changes in wall hydration and that it may be possible to exploit such mechanisms to improve crop resilience.

Glycoprotein conformation in plant cell walls

Planta ◽  
1979 ◽  
Vol 146 (2) ◽  
pp. 217-222 ◽  
Author(s):  
Roger B. Homer ◽  
Keith Roberts
Keyword(s):  
Plant Cell ◽  
Cell Walls ◽  
Plant Cell Walls

The Fine Structure of Fossil Plant Cell Walls

Science ◽  
1984 ◽  
Vol 225 (4662) ◽  
pp. 621-623 ◽  
Author(s):  
E. L. SMOOT ◽  
T. N. TAYLOR
Keyword(s):  
Fine Structure ◽  
Plant Cell ◽  
Cell Walls ◽  
Plant Cell Walls ◽  
Fossil Plant
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