Site-specific covalent labeling of His-tag fused proteins with a reactive Ni(ii)–NTA probe

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Keyword(s):  

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The use imidazolium tagged-mannosamine derivative for the non-covalent, rapid and site-specific labeling of sialic acid containing glycoproteins using commercial N-nitrilotriacetate fluorescent reagents in a range of live cells is reported.


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