Site-Specific Covalent Labeling of RNA by Enzymatic Transglycosylation

2015 ◽  
Vol 137 (40) ◽  
pp. 12756-12759 ◽  
Author(s):  
Seth C. Alexander ◽  
Kayla N. Busby ◽  
Christian M. Cole ◽  
Cun Yu Zhou ◽  
Neal K. Devaraj
Keyword(s):  
Covalent Labeling ◽  
Site Specific

scholarly journals An Efficient Site-Specific Method for Irreversible Covalent Labeling of Proteins with a Fluorophore

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Jiaquan Liu ◽  
Jeungphill Hanne ◽  
Brooke M. Britton ◽  
Matthew Shoffner ◽  
Aaron E. Albers ◽  
...  
Keyword(s):  
Specific Method ◽  
Covalent Labeling ◽  
Site Specific

scholarly journals Site-specific covalent labeling of His-tag fused proteins with N-acyl-N-alkyl sulfonamide reagent

2021 ◽  
Vol 30 ◽  
pp. 115947
Author(s):  
Vikram Thimaradka ◽  
Jae Hoon Oh ◽  
Christina Heroven ◽  
A. Radu Aricescu ◽  
Michisuke Yuzaki ◽  
...  
Keyword(s):  
Covalent Labeling ◽  
Site Specific ◽  
His Tag

Site-Specific, Covalent Labeling of Recombinant Antibody Fragments via Fusion to an Engineered Version of 6-O-Alkylguanine DNA Alkyltransferase

2009 ◽  
Vol 20 (5) ◽  
pp. 1010-1015 ◽  
Author(s):  
Florian Kampmeier ◽  
Markus Ribbert ◽  
Thomas Nachreiner ◽  
Sofia Dembski ◽  
Florent Beaufils ◽  
...  
Keyword(s):  
Recombinant Antibody ◽  
Antibody Fragments ◽  
Covalent Labeling ◽  
Site Specific ◽  
Recombinant Antibody Fragments ◽  
Dna Alkyltransferase

Site-specific covalent labeling of proteins inside live cells using small molecule probes

2009 ◽  
Vol 17 (3) ◽  
pp. 981-989 ◽  
Author(s):  
Souvik Chattopadhaya ◽  
Rajavel Srinivasan ◽  
Dawn S.Y. Yeo ◽  
Grace Y.J. Chen ◽  
Shao Q. Yao
Keyword(s):  
Small Molecule ◽  
Live Cells ◽  
Covalent Labeling ◽  
Site Specific ◽  
Small Molecule Probes

scholarly journals Imidazolium-tagged glycan probes for non-covalent labeling of live cells

2016 ◽  
Vol 52 (27) ◽  
pp. 4906-4909 ◽  
Author(s):  
David Benito-Alifonso ◽  
Shirley Tremell ◽  
Joanna C. Sadler ◽  
Monica Berry ◽  
M. Carmen Galan
Keyword(s):  
Sialic Acid ◽  
Live Cells ◽  
Covalent Labeling ◽  
Site Specific

The use imidazolium tagged-mannosamine derivative for the non-covalent, rapid and site-specific labeling of sialic acid containing glycoproteins using commercial N-nitrilotriacetate fluorescent reagents in a range of live cells is reported.

Site-specific covalent labeling of His-tag fused proteins with a reactive Ni(ii)–NTA probe

2009 ◽  
pp. 5880 ◽  
Author(s):  
Sho-hei Uchinomiya ◽  
Hiroshi Nonaka ◽  
Sho-hei Fujishima ◽  
Shinya Tsukiji ◽  
Akio Ojida ◽  
...  
Keyword(s):  
Covalent Labeling ◽  
Site Specific ◽  
His Tag

Large-cluster and combined fluorescent and gold immunoprobes

1996 ◽  
Vol 54 ◽  
pp. 892-893
Author(s):  
Richard D. Powell ◽  
James F. Hainfeld ◽  
Carol M. R. Halsey ◽  
David L. Spector ◽  
Shelley Kaurin ◽  
...  
Keyword(s):  
Metal Cluster ◽  
Sulfonyl Chloride ◽  
Gel Filtration ◽  
Cross Linking ◽  
Site Specific ◽  
Fab Fragments ◽  
Cluster Label ◽  
Covalently Linked ◽  
Texas Red ◽  
Fold Excess

Two new types of covalently linked, site-specific immunoprobes have been prepared using metal cluster labels, and used to stain components of cells. Combined fluorescein and 1.4 nm “Nanogold” labels were prepared by using the fluorescein-conjugated tris (aryl) phosphine ligand and the amino-substituted ligand in the synthesis of the Nanogold cluster. This cluster label was activated by reaction with a 60-fold excess of (sulfo-Succinimidyl-4-N-maleiniido-cyclohexane-l-carboxylate (sulfo-SMCC) at pH 7.5, separated from excess cross-linking reagent by gel filtration, and mixed in ten-fold excess with Goat Fab’ fragments against mouse IgG (obtained by reduction of F(ab’)2 fragments with 50 mM mercaptoethylamine hydrochloride). Labeled Fab’ fragments were isolated by gel filtration HPLC (Superose-12, Pharmacia). A combined Nanogold and Texas Red label was also prepared, using a Nanogold cluster derivatized with both and its protected analog: the cluster was reacted with an eight-fold excess of Texas Red sulfonyl chloride at pH 9.0, separated from excess Texas Red by gel filtration, then deprotected with HC1 in methanol to yield the amino-substituted label.

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