Microfluidic sample preparation: cell lysis and nucleic acid purification

2009 ◽  
Vol 1 (10) ◽  
pp. 574 ◽  
Author(s):  
Jungkyu Kim ◽  
Michael Johnson ◽  
Parker Hill ◽  
Bruce K. Gale
Keyword(s):  
Nucleic Acid ◽  
Sample Preparation ◽  
Cell Lysis ◽  
Nucleic Acid Purification

scholarly journals The implementation of a rapid sample preparation method for the detection of SARS-CoV-2 in a diagnostic laboratory in South Africa

2020 ◽  
Author(s):  
Gert Johannes Kruger Marais ◽  
Michelle Naidoo ◽  
Nei-yuan Hsiao ◽  
Ziyaad Valley-Omar ◽  
Heidi Smuts ◽  
...  
Keyword(s):  
South Africa ◽  
Nucleic Acid ◽  
Sample Preparation ◽  
Preparation Method ◽  
Preparation Technique ◽  
Rt Pcr ◽  
Sample Preparation Method ◽  
Diagnostic Laboratory ◽  
Percent Agreement ◽  
Nucleic Acid Purification

The SARS-CoV-2 pandemic has resulted in shortages of both critical reagents for nucleic acid purification and highly trained staff as supply chains are strained by high demand, public health measures and frequent quarantining and isolation of staff. This created the need for alternate workflows with limited reliance on specialised reagents, equipment and staff. We present here the validation and implementation of such a workflow for preparing samples for downstream SARS-CoV-2 RT-PCR using liquid handling robots. The rapid sample preparation technique evaluated, which included sample centrifugation and heating prior to RT-PCR, showed a 97.37% (95% CI: 92.55-99.28%) positive percent agreement and 97.30% (95% CI: 90.67-99.52%) negative percent agreement compared to nucleic acid purification-based testing. This method was subsequently adopted as the primary sample preparation method in the Groote Schuur Hospital Virology Diagnostic Laboratory in Cape Town, South Africa.

scholarly journals Nitrocellulose-bound achromopeptidase for point-of-care nucleic acid tests

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Georgios Chondrogiannis ◽  
Shirin Khaliliazar ◽  
Anna Toldrà ◽  
Pedro Réu ◽  
Mahiar M. Hamedi
Keyword(s):  
Nucleic Acid ◽  
Sample Preparation ◽  
Point Of Care ◽  
Dna Amplification ◽  
Recombinase Polymerase Amplification ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Enzymatic Function ◽  
Modern Biotechnology ◽  
Nucleic Acid Tests

AbstractEnzymes are the cornerstone of modern biotechnology. Achromopeptidase (ACP) is a well-known enzyme that hydrolyzes a number of proteins, notably proteins on the surface of Gram-positive bacteria. It is therefore used for sample preparation in nucleic acid tests. However, ACP inhibits DNA amplification which makes its integration difficult. Heat is commonly used to inactivate ACP, but it can be challenging to integrate heating into point-of-care devices. Here, we use recombinase polymerase amplification (RPA) together with ACP, and show that when ACP is immobilized on nitrocellulose paper, it retains its enzymatic function and can easily and rapidly be activated using agitation. The nitrocellulose-bound ACP does, however, not leak into the solution, preventing the need for deactivation through heat or by other means. Nitrocellulose-bound ACP thus opens new possibilities for paper-based Point-of-Care (POC) devices.

scholarly journals Final Report Nucleic Acid System - PCR, Multiplex Assays and Sample Preparation Project

2001 ◽  
Author(s):  
R P Koopman ◽  
R G Langlois ◽  
S Nasarabadi ◽  
W J Benett ◽  
J B Richards ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Sample Preparation ◽  
Final Report ◽  
Acid System ◽  
Multiplex Assays ◽  
Pcr Multiplex

scholarly journals Simple Synthesis of Functionalized Paramagnetic Beads for Nucleic Acid Purification and Manipulation

BIO-PROTOCOL ◽  
2019 ◽  
Vol 9 (20) ◽  
Author(s):  
Phil Oberacker ◽  
Peter Stepper ◽  
Donna Bond ◽  
Katharina Hipp ◽  
Timothy Hore ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Simple Synthesis ◽  
Nucleic Acid Purification ◽  
Paramagnetic Beads

Recent developments of magnetic beads for use in nucleic acid purification

1998 ◽  
Vol 816 (1) ◽  
pp. 107-111 ◽  
Author(s):  
Peter R Levison ◽  
Stephen E Badger ◽  
Jon Dennis ◽  
Prit Hathi ◽  
Martin J Davies ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Magnetic Beads ◽  
Recent Developments ◽  
Nucleic Acid Purification
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