scholarly journals Final Report Nucleic Acid System - PCR, Multiplex Assays and Sample Preparation Project

2001 ◽  
Author(s):  
R P Koopman ◽  
R G Langlois ◽  
S Nasarabadi ◽  
W J Benett ◽  
J B Richards ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Sample Preparation ◽  
Final Report ◽  
Acid System ◽  
Multiplex Assays ◽  
Pcr Multiplex

scholarly journals Final Report Nucleic Acid System - Hybrid PCR and Multiplex Assay Project Phase 2

2002 ◽  
Author(s):  
R P Koopman ◽  
R G Langlois ◽  
S Nasarabadi ◽  
W J Benett ◽  
B W Colston ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Multiplex Assay ◽  
Final Report ◽  
Phase 2 ◽  
Acid System

scholarly journals Nitrocellulose-bound achromopeptidase for point-of-care nucleic acid tests

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Georgios Chondrogiannis ◽  
Shirin Khaliliazar ◽  
Anna Toldrà ◽  
Pedro Réu ◽  
Mahiar M. Hamedi
Keyword(s):  
Nucleic Acid ◽  
Sample Preparation ◽  
Point Of Care ◽  
Dna Amplification ◽  
Recombinase Polymerase Amplification ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Enzymatic Function ◽  
Modern Biotechnology ◽  
Nucleic Acid Tests

AbstractEnzymes are the cornerstone of modern biotechnology. Achromopeptidase (ACP) is a well-known enzyme that hydrolyzes a number of proteins, notably proteins on the surface of Gram-positive bacteria. It is therefore used for sample preparation in nucleic acid tests. However, ACP inhibits DNA amplification which makes its integration difficult. Heat is commonly used to inactivate ACP, but it can be challenging to integrate heating into point-of-care devices. Here, we use recombinase polymerase amplification (RPA) together with ACP, and show that when ACP is immobilized on nitrocellulose paper, it retains its enzymatic function and can easily and rapidly be activated using agitation. The nitrocellulose-bound ACP does, however, not leak into the solution, preventing the need for deactivation through heat or by other means. Nitrocellulose-bound ACP thus opens new possibilities for paper-based Point-of-Care (POC) devices.

scholarly journals Magnetic Particles for Advanced Molecular Diagnosis

Materials ◽  
2019 ◽  
Vol 12 (13) ◽  
pp. 2158 ◽  
Author(s):  
Cristina Chircov ◽  
Alexandru Mihai Grumezescu ◽  
Alina Maria Holban
Keyword(s):  
Gene Expression ◽  
Infectious Disease ◽  
Nucleic Acid ◽  
Sample Preparation ◽  
Molecular Diagnosis ◽  
Magnetic Particles ◽  
Early Stage ◽  
Disease Diagnosis ◽  
Laboratory Medicine ◽  
Clinical Reality

Molecular diagnosis is the field that aims to develop nucleic-acid-based analytical methods for biological markers and gene expression assessments by combining laboratory medicine and molecular genetics. As it gradually becomes a clinical reality, molecular diagnosis could benefit from improvements resulting from thorough studies that could enhance the accuracy of these methods. The application of magnetic particles in molecular diagnosis tools has led to tremendous breakthroughs in terms of specificity, sensitivity, and discrimination in bioassays. Therefore, the aim of this review is to highlight the principles involved in the implementation of magnetic particles for sample preparation and targeted analyte isolation, purification, and extraction. Furthermore, the most recent advancements in the area of cancer and infectious disease diagnosis are presented, with an emphasis on screening and early stage detection.

P917 A new fast automated magnetic bead based nucleic acid sample preparation for STI diagnostics combined with BD ProbeTec TM ET

2007 ◽  
Vol 29 ◽  
pp. S239
Author(s):  
T. Engen ◽  
M. Anglès d'Auriac ◽  
S. Harja ◽  
M. Tauvo-Kallio ◽  
K. Rantakokko-Jalava ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Sample Preparation ◽  
Magnetic Bead ◽  
Acid Sample

The fluorescence enhancement of quercetin-nucleic acid system and the analytical application

Luminescence ◽  
2009 ◽  
pp. n/a-n/a
Author(s):  
Yuan Liu ◽  
Xia Wu ◽  
Haiping Zhou ◽  
Xiaoyu Liu ◽  
Fengju Zhang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Fluorescence Enhancement ◽  
Analytical Application ◽  
Acid System
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