Fluorescence resonance energy transfer between an anionic conjugated polymer and a dye-labeled lysozyme aptamer for specific lysozyme detection

2009 ◽  
pp. 2284 ◽  
Author(s):  
Jing Wang ◽  
Bin Liu
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Conjugated Polymer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Fluorescence Resonance

scholarly journals Highly Sensitive Detection of Melamine Based on the Fluorescence Resonance Energy Transfer between Conjugated Polymer Nanoparticles and Gold Nanoparticles

Polymers ◽  
2018 ◽  
Vol 10 (8) ◽  
pp. 873 ◽  
Author(s):  
Cui-jiao Zhang ◽  
Zhi-yan Gao ◽  
Qiu-bo Wang ◽  
Xian Zhang ◽  
Jin-shui Yao ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Conjugated Polymer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Polymer Nanoparticles ◽  
Highly Sensitive ◽  
Conjugated Polymer Nanoparticles ◽  
Fluorescence Resonance

Adding melamine as additives in food products will lead to many diseases and even death. However, the present techniques of melamine detection require time-consuming steps, complicated procedures and expensive analytical apparatus. The fluorescent assay method was facile and highly sensitive. In this work, a fluorescence resonance energy transfer (FRET) system for melamine detection was constructed based on conjugated polymer nanoparticles (CPNs) and gold nanoparticles (AuNPs). The energy transfer efficiency is up to 82.1%, and the system is highly selective and sensitive to melamine detection with a lower detection limit of 1.7 nmol/L. Moreover, the interaction mechanism was explored. The results showed that the fluorescence of CPNs were firstly quenched by AuNPs, and then restored after adding melamine because of reducing FRET between CPNs and AuNPs. Lastly, the proposed method was carried out for melamine detection in powdered infant formula with satisfactory results.

scholarly journals Detection of Streptavidin Based on Terminal Protection and Cationic Conjugated Polymer-Mediated Fluorescence Resonance Energy Transfer

Polymers ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 725 ◽  
Author(s):  
Tingting Hu ◽  
Ying Yan ◽  
Zhenwei Tang ◽  
Xinfa Liu ◽  
Changbei Ma
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Conjugated Polymer ◽  
Electrostatic Interactions ◽  
Detection System ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Dna Probe ◽  
Transfer Energy ◽  
Fluorescence Resonance

In this paper, a fast and simple strategy for sensitive detection of streptavidin (SA) was proposed based on terminal protection of small molecule-linked DNA and cationic conjugated polymer-mediated fluorescence resonance energy transfer (FRET). In principle, we designed a biotin-labelled DNA probe (P1) as the recognitive probe of SA, along with a complementary DNA probe (P2) to form double-stranded DNA (dsDNA) with P1. SYBR Green I (SG I) as a fluorescent dye was further used to specifically bind to dsDNA to emit stronger fluorescence. The cationic poly[(9,9-bis(6′-N,N,N-triethy-lammonium)hexyl) fluorenylene phenylene dibromide] (PFP) acted as the donor to participate in the FRET and transfer energy to the recipient SG I. In the absence of SA, P1 could not hybridize with P2 to form dsDNA and was digested by exonuclease I (Exo I); thus, only a weak FRET signal would be observed. In the presence of SA, biotin could specifically bind to SA, which protected P1 from Exo I cleavage. Then, P1 and P2 were hybridized into dsDNA. Therefore, the addition of SG I and PFP led to obvious FRET signal due to strong electrostatic interactions. Then, SA can be quantitatively detected by monitoring FRET changes. As the whole reagent reaction was carried out in 1.5 mL EP and detected in the colorimetric dish, the operation process of the detection system was relatively simple. The response time for each step was also relatively short. In this detection system, the linear equation was obtained for SA from 0.1 to 20 nM with a low detection limit of 0.068 nM (S/N = 3). In addition, this strategy has also achieved satisfactory results in the application of biological samples, which reveals the application prospect of this method in the future.

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