Water-Soluble Conjugated Polymer as a Platform for Adenosine Deaminase Sensing Based on Fluorescence Resonance Energy Transfer Technique

2014 ◽  
Vol 86 (13) ◽  
pp. 6433-6438 ◽  
Author(s):  
Chun Wang ◽  
Yanli Tang ◽  
Yue Liu ◽  
Yang Guo
Keyword(s):  
Energy Transfer ◽  
Adenosine Deaminase ◽  
Fluorescence Resonance Energy Transfer ◽  
Conjugated Polymer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Water Soluble ◽  
Fluorescence Resonance

scholarly journals Peptide-coated palladium nanoparticle for highly sensitive bioanalysis of trypsin in human urine samples

2018 ◽  
Vol 8 ◽  
pp. 184798041882039 ◽  
Author(s):  
Guohua Zhou ◽  
Huimin Jiang ◽  
Yanfang Zhou ◽  
Peilian Liu ◽  
Yongmei Jia ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Palladium Nanoparticles ◽  
Limit Of Detection ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Water Soluble ◽  
Urine Samples ◽  
Fluorescence Resonance ◽  
Functional Peptide

In recent years, palladium nanoparticles have been proved as energy acceptor candidates in fluorescence resonance energy transfer-based sensors for analytical and biological purposes. In this article, peptide-coated palladium nanoparticles were prepared using a simple one-step preparation method. The peptide Cys-Ala-Leu-Asn-Asn was used as a ligand, whereas hydrazine hydrate was used as a reductant to obtain water-soluble and stable peptide-coated palladium nanoparticles. Additionally, peptide-coated palladium nanoparticles were functionalized by adding the functional peptide CALNNGGARK(FITC) in combination with Cys-Ala-Leu-Asn-Asn during the preparation process. The prepared functionalized peptide-coated palladium nanoparticles were used for trypsin detection based on the fluorescence resonance energy transfer approach. Under optimized conditions, the proposed method can be used for the detection of trypsin concentrations in the range of approximately 0.2–8-μg/mL with a limit of detection of 0.18-μg/mL. The functionalized peptide-coated palladium nanoparticles were successfully applied for the detection of trypsin in urine samples. Our findings also indicated that peptide-coated palladium nanoparticles can highly quench fluorophores and are suitable for the manufacture of off–on state fluorescent sensors. We anticipated that the peptide-coated palladium nanoparticles proposed in this article will have great potential for the detection of trypsin in urine and other analytical, biological, and clinical applications.

FLUORESCENCE RESONANCE ENERGY TRANSFER BETWEEN DYES AND WATER-SOLUBLE QUANTUM DOTS WITH AVIDIN AS A BRIDGE

2004 ◽  
Vol 03 (03) ◽  
pp. 273-280
Author(s):  
QI-DAN CHEN ◽  
ZHANG-BI LIN ◽  
XING-GUANG SU ◽  
HAO ZHANG ◽  
XIAO-HONG HE ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Resonance Energy Transfer ◽  
Fluorescence Emission ◽  
Resonance Energy ◽  
Water Soluble ◽  
Fluorescence Emission Spectrum ◽  
Fluorescence Characteristics ◽  
Fluorescence Resonance

3-Mercaptopropyl acid-capped quantum dots (QDs) synthesized in aqueous solution were coupled to avidin-sulforhodamine, also named avidin-Texas red (ATR), via electrostatic attraction. An intensity reduction in the fluorescence emission spectrum of QDs and an enhanced fluorescence intensity of the dye were observed on account of fluorescence resonance energy transfer from the QD donors to the dye acceptors. In addition, the fluorescence characteristics of the QD-ATR conjugates were strongly-related to the quantity of ATR, pH value and ionic strength.

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