Drop-based microfluidic devices for encapsulation of single cells

Lab on a Chip ◽  
2008 ◽  
Vol 8 (7) ◽  
pp. 1110 ◽  
Author(s):  
Sarah Köster ◽  
Francesco E. Angilè ◽  
Honey Duan ◽  
Jeremy J. Agresti ◽  
Anton Wintner ◽  
...  
Keyword(s):  
Single Cells ◽  
Microfluidic Devices

scholarly journals Microfluidic devices for measuring gene network dynamics in single cells

2009 ◽  
Vol 10 (9) ◽  
pp. 628-638 ◽  
Author(s):  
Matthew R. Bennett ◽  
Jeff Hasty
Keyword(s):  
Gene Network ◽  
Single Cells ◽  
Network Dynamics ◽  
Microfluidic Devices

Microfluidic Devices for the Analysis of Single Cells: Leaving No Protein Uncounted

2007 ◽  
Vol 2007 (388) ◽  
pp. pe29-pe29 ◽  
Author(s):  
M. Navratil ◽  
C. E. Whiting ◽  
E. A. Arriaga
Keyword(s):  
Single Cells ◽  
Microfluidic Devices

High-throughput, deterministic single cell trapping and long-term clonal cell culture in microfluidic devices

Lab on a Chip ◽  
2015 ◽  
Vol 15 (4) ◽  
pp. 1072-1083 ◽  
Author(s):  
Huaying Chen ◽  
Jane Sun ◽  
Ernst Wolvetang ◽  
Justin Cooper-White
Keyword(s):  
High Throughput ◽  
Clonal Growth ◽  
Single Cells ◽  
Microfluidic Devices ◽  
Design Development ◽  
Cell Trapping ◽  
Single Cell Trapping ◽  
Development And Validation

In this paper, the design, development and validation of a novel high throughput microfluidic device enabling both the robust and rapid trapping of 100's to 1000's of single cells and their in situ clonal growth is described.

scholarly journals Sequential Cell-Processing System by Integrating Hydrodynamic Purification and Dielectrophoretic Trapping for Analyses of Suspended Cancer Cells

Micromachines ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 47 ◽  
Author(s):  
Jongho Park ◽  
Takayuki Komori ◽  
Toru Uda ◽  
Keiichi Miyajima ◽  
Teruo Fujii ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Lateral Displacement ◽  
Single Cells ◽  
Processing System ◽  
Microfluidic Devices ◽  
Integrated System ◽  
Suspended Cell ◽  
Cell Processing ◽  
Microwell Array ◽  
Complex Preparation

Microfluidic devices employing dielectrophoresis (DEP) have been widely studied and applied in the manipulation and analysis of single cells. However, several pre-processing steps, such as the preparation of purified target samples and buffer exchanges, are necessary to utilize DEP forces for suspended cell samples. In this paper, a sequential cell-processing device, which is composed of pre-processing modules that employ deterministic lateral displacement (DLD) and a single-cell trapping device employing an electroactive microwell array (EMA), is proposed to perform the medium exchange followed by arraying single cells sequentially using DEP. Two original microfluidic devices were efficiently integrated by using the interconnecting substrate containing rubber gaskets that tightly connect the inlet and outlet of each device. Prostate cancer cells (PC3) suspended in phosphate-buffered saline buffer mixed with microbeads were separated and then resuspended into the DEP buffer in the integrated system. Thereafter, purified PC3 cells were trapped in a microwell array by using the positive DEP force. The achieved separation and trapping efficiencies exceeded 94% and 93%, respectively, when using the integrated processing system. This study demonstrates an integrated microfluidic device by processing suspended cell samples, without the requirement of complex preparation steps.

Single-cell assay on microfluidic devices

The Analyst ◽  
2019 ◽  
Vol 144 (3) ◽  
pp. 808-823 ◽  
Author(s):  
Qiushi Huang ◽  
Sifeng Mao ◽  
Mashooq Khan ◽  
Jin-Ming Lin
Keyword(s):  
Single Cell ◽  
Single Cells ◽  
Microfluidic Devices ◽  
Cell Populations ◽  
Cell Assay

Advances in microfluidic techniques have prompted researchers to study the inherent heterogeneity of single cells in cell populations.

Probing single cells using flow in microfluidic devices

2012 ◽  
Vol 204 (1) ◽  
pp. 85-101 ◽  
Author(s):  
D. Qi ◽  
D. J. Hoelzle ◽  
A. C. Rowat
Keyword(s):  
Single Cells ◽  
Microfluidic Devices

scholarly journals Optofluidics for handling and analysis of single living cells

2017 ◽  
Vol 4 (1) ◽  
Author(s):  
Gerardo Perozziello ◽  
Patrizio Candeloro ◽  
Maria Laura Coluccio ◽  
Enzo Di Fabrizio
Keyword(s):  
Analytical Chemistry ◽  
Single Cells ◽  
Microfluidic Devices ◽  
Photonic Devices ◽  
Living Cells ◽  
Optical Elements ◽  
Integrated Optical ◽  
Single Living Cells ◽  
Integrated Optical Sensor ◽  
Single Living

AbstractOptofluidics is a field with important applications in areas such as biotechnology, chemical synthesis and analytical chemistry. Optofluidic devices combine optical elements into microfluidic devices in ways that increase portability and sensitivity of analysis for diagnostic or screening purposes .In fact in these devices fluids give fine adaptability, mobility and accessibility to nanoscale photonic devices which otherwise could not be realized using conventional devices. This review describes several cases inwhich optical or microfluidic approaches are used to trap single cells in proximity of integrated optical sensor for being analysed.

scholarly journals Paper-Thin Multilayer Microfluidic Devices with Integrated Valves

2020 ◽  
Author(s):  
Soohong Kim ◽  
Gabriel Dorlhiac ◽  
Rodrigo Cotrim Chaves ◽  
Mansi Zalavadia ◽  
Aaron Streets
Keyword(s):  
High Resolution ◽  
Raman Scattering ◽  
Soft Lithography ◽  
Stimulated Raman Scattering ◽  
Single Cells ◽  
Microfluidic Devices ◽  
Fluorescent Imaging ◽  
Cover Glass ◽  
On Chip ◽  
Stimulated Raman

Integrated valve microfluidics has an unparalleled capability to automate the rapid delivery of fluids at the nanoliter scale for high-throughput biological experimentation. However, multilayer soft lithography, which is used to fabricate valve-microfluidics, produces devices with a minimum thickness of around five millimeters. This form-factor limitation prevents the use of such devices in experiments with limited sample thickness tolerance such as 4-pi microscopy, stimulated Raman scattering microscopy, and many forms of optical or magnetic tweezer applications. We present a new generation of integrated valve microfluidic devices that are less than 300 μm thick, including the cover-glass substrate, that resolves the thickness limitation. This "thin-chip" was fabricated through a novel soft-lithography technique that produces on-chip micro-valves with the same functionality and reliability of traditional thick valve-microfluidic devices despite the orders of magnitude reduction in thickness. We demonstrated the advantage of using our thin-chip over traditional thick devices to automate fluid control while imaging on a high-resolution inverted microscope. First, we demonstrate that the thin-chip provides improved signal to noise when imaging single cells with two-color stimulated Raman scattering (SRS). We then demonstrated how the thin-chip can be used to simultaneously perform on-chip magnetic manipulation of beads and fluorescent imaging. This study reveals the potential of our thin-chip in high-resolution imaging, sorting, and bead capture-based single-cell multi-omics applications.

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