Mass spectrometric analysis of cross-linking sites for the structure of proteins and protein complexes

2008 ◽  
Vol 4 (8) ◽  
pp. 816 ◽  
Author(s):  
Young Jin Lee
Keyword(s):  
Protein Complexes ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Cross Linking ◽  
Spectrometric Analysis ◽  
Structure Of Proteins

A new crosslinker for mass spectrometric analysis of the quaternary structure of protein complexes

2001 ◽  
Vol 12 (2) ◽  
pp. 222-227 ◽  
Author(s):  
J. W. Back ◽  
A. F. Hartog ◽  
H. L. Dekker ◽  
Anton O. Muijsers ◽  
L. J. Koning ◽  
...  
Keyword(s):  
Quaternary Structure ◽  
Protein Complexes ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis

scholarly journals SECAT: Quantifying differential protein-protein interaction states by network-centric analysis

2019 ◽  
Author(s):  
George Rosenberger ◽  
Moritz Heusel ◽  
Isabell Bludau ◽  
Ben Collins ◽  
Claudia Martelli ◽  
...  
Keyword(s):  
Protein Interaction ◽  
Protein Interactions ◽  
Molecular Mechanisms ◽  
Protein Complexes ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Size Exclusion ◽  
Spectrometric Analysis ◽  
Differential Analysis ◽  
Ppi Networks

AbstractProtein-protein interactions (PPIs) play critical functional and regulatory roles in virtually all cellular processes. They are essential for the formation of macromolecular complexes, which in turn constitute the basis for extended protein interaction networks that determine the functional state of a cell. We and others have previously shown that chromatographic fractionation of native protein complexes in combination with bottom-up mass spectrometric analysis of consecutive fractions supports the multiplexed characterization and detection of state-specific changes of protein complexes.In this study, we describe a computational approach that extends the analysis of data from the co-fractionation / mass spectrometric analysis of native complexes to the level of PPI networks, thus enabling a qualitative and quantitative comparison of the proteome organization between samples and states. The Size-Exclusion Chromatography Algorithmic Toolkit (SECAT) implements a novel, network-centric strategy for the scalable and robust differential analysis of PPI networks. SECAT and its underlying statistical framework elucidate differential quantitative abundance and stoichiometry attributes of proteins in the context of their PPIs. We validate algorithm predictions using publicly available datasets and demonstrate that SECAT represents a more scalable and effective methodology to assess protein-network state and that our approach thus obviates the need to explicitly infer individual protein complexes. Further, by differential analysis of PPI networks of HeLa cells in interphase and mitotic state, respectively, we demonstrate the ability of the algorithm to detect PPI network differences and to thus suggest molecular mechanisms that differentiate cellular states.

Bioconjugation of Ribonuclease A:  A Detailed Chromatographic and Mass Spectrometric Analysis of Chemical Modification by a Cross-Linking Reagent

2000 ◽  
Vol 11 (2) ◽  
pp. 182-194 ◽  
Author(s):  
T. Joy Méndez ◽  
Jodie V. Johnson ◽  
Linda S. Nichols ◽  
G. H. Lisa Lang ◽  
John R. Eyler ◽  
...  
Keyword(s):  
Chemical Modification ◽  
Ribonuclease A ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Cross Linking ◽  
Spectrometric Analysis

Computer-assisted mass spectrometric analysis of naturally occurring and artificially introduced cross-links in proteins and protein complexes

FEBS Journal ◽  
2006 ◽  
Vol 273 (2) ◽  
pp. 281-291 ◽  
Author(s):  
Leo J. Koning ◽  
Piotr T. Kasper ◽  
Jaap Willem Back ◽  
Merel A. Nessen ◽  
Frank Vanrobaeys ◽  
...  
Keyword(s):  
Protein Complexes ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis ◽  
Computer Assisted ◽  
Naturally Occurring ◽  
Cross Links

A Questionnaire Survey on General Status and Opinions about Clinical Mass Spectrometric Analysis in Korea (2018)

2019 ◽  
Vol 9 (3) ◽  
pp. 161
Author(s):  
Sung-Eun Cho ◽  
Hyojin Chae ◽  
Hyung-Doo Park ◽  
Sail Chun ◽  
Yong-Wha Lee ◽  
...  
Keyword(s):  
Questionnaire Survey ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis ◽  
General Status

Mechanisms of Dopant Depth Profile Modification During Mass Spectrometric Analysis of Multilayer Structure

2015 ◽  
Vol 60 (6) ◽  
pp. 511-520 ◽  
Author(s):  
A.A. Efremov ◽  
◽  
V.G. Litovchenko ◽  
V.P. Melnik ◽  
O.S. Oberemok ◽  
...  
Keyword(s):  
Depth Profile ◽  
Multilayer Structure ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis ◽  
Profile Modification
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