Physico-chemical and structural properties of hydrogels formed by chitosan, in the presence and absence of poly(vinylpyrrolidone) and sodium decylsulfate

2007 ◽  
Vol 9 (46) ◽  
pp. 6150 ◽  
Author(s):  
Gaetano Mangiapia ◽  
Henrich Frielinghaus ◽  
Gerardino D’Errico ◽  
Ornella Ortona ◽  
Roberto Sartorio ◽  
...  
2019 ◽  
Vol 47 (12) ◽  
pp. 6195-6207 ◽  
Author(s):  
Marius Socol ◽  
Renjie Wang ◽  
Daniel Jost ◽  
Pascal Carrivain ◽  
Cédric Vaillant ◽  
...  

Abstract DNA folding and dynamics along with major nuclear functions are determined by chromosome structural properties, which remain, thus far, elusive in vivo. Here, we combine polymer modeling and single particle tracking experiments to determine the physico-chemical parameters of chromatin in vitro and in living yeast. We find that the motion of reconstituted chromatin fibers can be recapitulated by the Rouse model using mechanical parameters of nucleosome arrays deduced from structural simulations. Conversely, we report that the Rouse model shows some inconsistencies to analyze the motion and structural properties inferred from yeast chromosomes determined with chromosome conformation capture techniques (specifically, Hi-C). We hence introduce the Rouse model with Transient Internal Contacts (RouseTIC), in which random association and dissociation occurs along the chromosome contour. The parametrization of this model by fitting motion and Hi-C data allows us to measure the kinetic parameters of the contact formation reaction. Chromosome contacts appear to be transient; associated to a lifetime of seconds and characterized by an attractive energy of –0.3 to –0.5 kBT. We suggest attributing this energy to the occurrence of histone tail-DNA contacts and notice that its amplitude sets chromosomes in ‘theta’ conditions, in which they are poised for compartmentalization and phase separation.


2019 ◽  
Vol 274 ◽  
pp. 281-290 ◽  
Author(s):  
Maria Paciulli ◽  
Massimiliano Rinaldi ◽  
Margherita Rodolfi ◽  
Tommaso Ganino ◽  
Michele Morbarigazzi ◽  
...  

2007 ◽  
Vol 82 (3) ◽  
pp. 383-394 ◽  
Author(s):  
Lovedeep Kaur ◽  
Jaspreet Singh ◽  
Owen J. McCarthy ◽  
Harmit Singh

2016 ◽  
Vol 87 ◽  
pp. 514-521 ◽  
Author(s):  
Adil Gani ◽  
Amreen Jan ◽  
Asima Shah ◽  
F.A. Masoodi ◽  
Mudasir Ahmad ◽  
...  

2004 ◽  
Vol 7 (2) ◽  
Author(s):  
Amjad H. El-Sheikh ◽  
Maha F. Tutunji ◽  
Ghazi A. W. Derwish ◽  
Salem M. Musleh

AbstractMetal oxides of titanium, vanadium, manganese and iron were deposited on the native Jordanian diatomaceous clay. The chemical and structural properties of the prepared samples were investigated using SEM, XRF, ICP, XRD and FTIR techniques. The diatomaceous clay supported metal oxides were used in preliminary experiments to explore their interaction with aqueous phenol solution under thermal and photochemical conditions, in the presence and absence of hydrogen peroxide. The results showed that the metal oxides have interacted with the diatomaceous clay and caused some structural changes. These were reflected in the surface area of the samples and their activity in the phenol reactions.


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