One step visual detection of PCR products with gold nanoparticles and a nucleic acid lateral flow (NALF) device

2007 ◽  
pp. 4251 ◽  
Author(s):  
Jenny Aveyard ◽  
Maryam Mehrabi ◽  
Andrew Cossins ◽  
Helen Braven ◽  
Robert Wilson
Keyword(s):  
Gold Nanoparticles ◽  
Nucleic Acid ◽  
Visual Detection ◽  
Lateral Flow ◽  
Pcr Products ◽  
One Step

Development of a nucleic acid lateral flow strip for rapid, visual detection of Nosema bombycis in silkworm eggs

2019 ◽  
Vol 164 ◽  
pp. 59-65 ◽  
Author(s):  
Zhangshuai He ◽  
Qi Ni ◽  
Yue Song ◽  
Rui Wang ◽  
Yunlin Tang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Visual Detection ◽  
Lateral Flow ◽  
Lateral Flow Strip ◽  
Nosema Bombycis

scholarly journals Rapid label-free visual detection of KRAS mutations using peptide nucleic acid and unmodified gold nanoparticles

RSC Advances ◽  
2017 ◽  
Vol 7 (77) ◽  
pp. 48554-48560 ◽  
Author(s):  
Xihong Zhao ◽  
Chii-Wann Lin
Keyword(s):  
Colorectal Cancer ◽  
Gold Nanoparticles ◽  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Visual Detection ◽  
Label Free ◽  
Kras Mutations

Colorectal cancer (CRC) is among the most commonly diagnosed cancers worldwide.

Ultrasensitive visual detection of Hg2+ ions via the Tyndall effect of gold nanoparticles

2021 ◽  
Author(s):  
Zihao Deng ◽  
Wenying Jin ◽  
Qiaobo Yin ◽  
Jinkun Huang ◽  
Ziheng Huang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Visual Detection ◽  
Eye Detection ◽  
Naked Eye ◽  
Tyndall Effect ◽  
One Step ◽  
Naked Eye Detection

This work describes a new nanosensor for one-step ultrasensitive naked-eye detection of Hg2+ ions based on the target-triggered aggregation of gold nanoparticles showing a dramatically enhanced Tyndall effect.

scholarly journals Visual Detection of Dengue-1 RNA Using Gold Nanoparticle-Based Lateral Flow Biosensor

Diagnostics ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 74 ◽  
Author(s):  
Flora M. Yrad ◽  
Josephine M. Castañares ◽  
Evangelyn C. Alocilja
Keyword(s):  
Nucleic Acid ◽  
Gold Nanoparticle ◽  
Disease Surveillance ◽  
Visual Detection ◽  
Dengue Infection ◽  
Viral Disease ◽  
Lateral Flow ◽  
Resource Limited ◽  
Sandwich Type ◽  
Human Sera

Dengue is a rapidly spreading mosquito-borne viral disease. Early diagnosis is important for clinical screening, medical management, and disease surveillance. The objective of this study was to develop a colorimetric lateral flow biosensor (LFB) for the visual detection of dengue-1 RNA using dextrin-capped gold nanoparticle (AuNP) as label. The detection was based on nucleic acid sandwich-type hybridization among AuNP-labeled DNA reporter probe, dengue-1 target RNA, and dengue-1 specific DNA capture probe immobilized on the nitrocellulose membrane. Positive test generated a red test line on the LFB strip, which enabled visual detection. The optimized biosensor has a cut-off value of 0.01 µM using synthetic dengue-1 target. Proof-of-concept application of the biosensor detected dengue-1 virus in pooled human sera with a cut-off value of 1.2 × 104 pfu/mL. The extracted viral RNA, when coupled with nucleic acid sequence-based amplification (NASBA), was detected on the LFB in 20 min. This study first demonstrates the applicability of dextrin-capped AuNP as label for lateral flow assay. The biosensor being developed provides a promising diagnostic platform for early detection of dengue infection in high-risk resource-limited areas.

scholarly journals Simultaneous Detection of Nucleic Acid and Protein Using Gold Nanoparticles and Lateral Flow Device

2014 ◽  
Vol 30 (6) ◽  
pp. 637-642 ◽  
Author(s):  
Xun MAO ◽  
Anant GURUNG ◽  
Hui XU ◽  
Meenu BALODA ◽  
Yuqing HE ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Nucleic Acid ◽  
Simultaneous Detection ◽  
Lateral Flow ◽  
Lateral Flow Device ◽  
Flow Device

Point-of-care and visual detection of P. aeruginosa and its toxin genes by multiple LAMP and lateral flow nucleic acid biosensor

2016 ◽  
Vol 81 ◽  
pp. 317-323 ◽  
Author(s):  
Yuting Chen ◽  
Nan Cheng ◽  
Yuancong Xu ◽  
Kunlun Huang ◽  
Yunbo Luo ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Visual Detection ◽  
Point Of Care ◽  
Lateral Flow ◽  
Toxin Genes
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