In vitro biotransformation of an arsenosugar by mouse anaerobic cecal microflora and cecal tissue as examined using IC-ICP-MS and LC-ESI-MS/MS

The Analyst ◽  
2006 ◽  
Vol 131 (5) ◽  
pp. 648 ◽  
Author(s):  
Sean D. Conklin ◽  
Amanda H. Ackerman ◽  
Michael W. Fricke ◽  
Patricia A. Creed ◽  
John T. Creed ◽  
...  
Keyword(s):  
Esi Ms ◽  
Icp Ms ◽  
Cecal Microflora

Two-dimensional HPLC coupled to ICP-MS and electrospray ionisation (ESI)-MS/MS for investigating the bioavailability in vitro of arsenic species from edible seaweed

2011 ◽  
Vol 402 (10) ◽  
pp. 3359-3369 ◽  
Author(s):  
Cristina Garcia-Sartal ◽  
Sutthinun Taebunpakul ◽  
Emma Stokes ◽  
María del Carmen Barciela-Alonso ◽  
Pilar Bermejo-Barrera ◽  
...  
Keyword(s):  
Arsenic Species ◽  
Two Dimensional ◽  
Edible Seaweed ◽  
Esi Ms ◽  
Electrospray Ionisation ◽  
Icp Ms

In vitro biotransformation of dimethylarsinic acid and trimethylarsine oxide by anaerobic microflora of mouse cecum analyzed by HPLC-ICP-MS and HPLC-ESI-MS

2009 ◽  
Vol 24 (8) ◽  
pp. 1062 ◽  
Author(s):  
Kevin M. Kubachka ◽  
Michael C. Kohan ◽  
Sean D. Conklin ◽  
Karen Herbin-Davis ◽  
John T. Creed ◽  
...  
Keyword(s):  
Dimethylarsinic Acid ◽  
Esi Ms ◽  
Icp Ms ◽  
Mouse Cecum ◽  
Trimethylarsine Oxide ◽  
Anaerobic Microflora

Exploring the in vitro formation of trimethylarsine sulfide from dimethylthioarsinic acid in anaerobic microflora of mouse cecum using HPLC–ICP-MS and HPLC–ESI-MS

2009 ◽  
Vol 239 (2) ◽  
pp. 137-143 ◽  
Author(s):  
Kevin M. Kubachka ◽  
Michael C. Kohan ◽  
Karen Herbin-Davis ◽  
John T. Creed ◽  
David J. Thomas
Keyword(s):  
Esi Ms ◽  
Icp Ms ◽  
Mouse Cecum ◽  
Anaerobic Microflora

scholarly journals Pharmacoinformatics and UPLC-QTOF/ESI-MS-Based Phytochemical Screening of Combretum indicum against Oxidative Stress and Alloxan-Induced Diabetes in Long–Evans Rats

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4634
Author(s):  
Md. Shaekh Forid ◽  
Md. Atiar Rahman ◽  
Mohd Fadhlizil Fasihi Mohd Aluwi ◽  
Md. Nazim Uddin ◽  
Tapashi Ghosh Roy ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Immune Modulation ◽  
Computational Study ◽  
Density Lipoprotein ◽  
Control Group ◽  
Esi Ms ◽  
Long Evans ◽  
Antidiabetic Effects

This research investigated a UPLC-QTOF/ESI-MS-based phytochemical profiling of Combretum indicum leaf extract (CILEx), and explored its in vitro antioxidant and in vivo antidiabetic effects in a Long–Evans rat model. After a one-week intervention, the animals’ blood glucose, lipid profile, and pancreatic architectures were evaluated. UPLC-QTOF/ESI-MS fragmentation of CILEx and its eight docking-guided compounds were further dissected to evaluate their roles using bioinformatics-based network pharmacological tools. Results showed a very promising antioxidative effect of CILEx. Both doses of CILEx were found to significantly (p < 0.05) reduce blood glucose, low-density lipoprotein (LDL), and total cholesterol (TC), and increase high-density lipoprotein (HDL). Pancreatic tissue architectures were much improved compared to the diabetic control group. A computational approach revealed that schizonepetoside E, melianol, leucodelphinidin, and arbutin were highly suitable for further therapeutic assessment. Arbutin, in a Gene Ontology and PPI network study, evolved as the most prospective constituent for 203 target proteins of 48 KEGG pathways regulating immune modulation and insulin secretion to control diabetes. The fragmentation mechanisms of the compounds are consistent with the obtained effects for CILEx. Results show that the natural compounds from CILEx could exert potential antidiabetic effects through in vivo and computational study.

scholarly journals An ICP-MS, ESI-MS and molecular modelling investigation of homogeneous gallium affinity tagging (HMAT) of phosphopeptides

2013 ◽  
Vol 341-342 ◽  
pp. 18-27 ◽  
Author(s):  
Claire L. Camp ◽  
Eslam M. Moustafa ◽  
Helen J. Reid ◽  
Barry L. Sharp ◽  
Tamer Shoeib
Keyword(s):  
Molecular Modelling ◽  
Esi Ms ◽  
Icp Ms

scholarly journals Determination of Specific Iron Chelator by Using LC-ICP-MS and LC-ESI-MS

2015 ◽  
Vol 30 ◽  
pp. 256-261 ◽  
Author(s):  
Khairul Nizam Mohamed ◽  
Martha Gledhill
Keyword(s):  
Iron Chelator ◽  
Esi Ms ◽  
Icp Ms

GC-MS and LC-ESI-MS analysis of biologically active fractions from Verbascum letourneuxii; efficient protocol for in vitro propagation

2020 ◽  
Vol 29 ◽  
pp. 101817
Author(s):  
Mai M. Farid ◽  
Alia Y. Ragheb ◽  
Mona El-Shabrawy ◽  
Mona M. Marzouk ◽  
Sameh R. Hussein ◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Biologically Active ◽  
Esi Ms ◽  
Ms Analysis

scholarly journals In Vitro Anti-Diabetic Activities and UHPLC-ESI-MS/MS Profile of Muntingia calabura Leaves Extract

Molecules ◽  
2022 ◽  
Vol 27 (1) ◽  
pp. 287
Author(s):  
Nur Khaleeda Zulaikha Zolkeflee ◽  
Nurul Shazini Ramli ◽  
Azrina Azlan ◽  
Faridah Abas
Keyword(s):  
Published Data ◽  
Drying Methods ◽  
Esi Ms ◽  
Synthetic Drugs ◽  
Ic50 Values ◽  
Phytochemical Profile ◽  
Ultrahigh Performance Liquid Chromatography ◽  
Diabetic Treatment ◽  
Muntingia Calabura

Anti-diabetic compounds from natural sources are now being preferred to prevent or treat diabetes due to adverse effects of synthetic drugs. The decoction of Muntingia calabura leaves was traditionally consumed for diabetes treatment. However, there has not been any published data currently available on the processing effects on this plant’s biological activity and phytochemical profile. Therefore, this study aims to evaluate the effect of three drying methods (freeze-drying (FD), air-drying (AD), and oven-drying (OD)) and ethanol:water ratios (0, 50, and 100%) on in vitro anti-diabetic activities of M. calabura leaves. In addition, an ultrahigh-performance-liquid chromatography–electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS) method was used to characterize the metabolites in the active extract. The FD M. calabura leaves, extracted with 50% ethanol, is the most active extract that exhibits a high α-glucosidase and α-amylase inhibitory activities with IC50 values of 0.46 ± 0.05 and 26.39 ± 3.93 µg/mL, respectively. Sixty-one compounds were tentatively identified by using UHPLC-ESI-MS/MS from the most active extract. Quantitative analysis, by using UHPLC, revealed that geniposide, daidzein, quercitrin, 6-hydroxyflavanone, kaempferol, and formononetin were predominant compounds identified from the active extract. The results have laid down preliminary steps toward developing M. calabura leaves extract as a potential source of bioactive compounds for diabetic treatment.

scholarly journals Evaluation of anticancer activity in vitro of a stable copper(I) complex with phosphine-peptide conjugate

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Urszula K. Komarnicka ◽  
Barbara Pucelik ◽  
Daria Wojtala ◽  
Monika K. Lesiów ◽  
Grażyna Stochel ◽  
...  
Keyword(s):  
A549 Cells ◽  
Ros Generation ◽  
Dependent Manner ◽  
Hacat Cells ◽  
Icp Ms ◽  
Intracellular Ros ◽  
M Phase ◽  
Lung Adenocarcinoma A549 ◽  
Induced Apoptosis

Abstract[CuI(2,9-dimethyl-1,10-phenanthroline)P(p-OCH3-Ph)2CH2SarcosineGlycine] (1-MPSG), highly stable in physiological media phosphino copper(I) complex—is proposed herein as a viable alternative to anticancer platinum-based drugs. It is noteworthy that, 1-MPSG significantly and selectively reduced cell viability in a 3D spheroidal model of human lung adenocarcinoma (A549), in comparison with non-cancerous HaCaT cells. Confocal microscopy and an ICP-MS analysis showed that 1-MPSG effectively accumulates inside A549 cells with colocalization in mitochondria and nuclei. A precise cytometric analysis revealed a predominance of apoptosis over the other types of cell death. In the case of HaCaT cells, the overall cytotoxicity was significantly lower, indicating the selective activity of 1-MPSG towards cancer cells. Apoptosis also manifested itself in a decrease in mitochondrial membrane potential along with the activation of caspases-3/9. Moreover, the caspase inhibitor (Z-VAD-FMK) pretreatment led to decreased level of apoptosis (more pronouncedly in A549 cells than in non-cancerous HaCaT cells) and further validated the caspases dependence in 1-MPSG-induced apoptosis. Furthermore, the 1-MPSG complex presumably induces the changes in the cell cycle leading to G2/M phase arrest in a dose-dependent manner. It was also observed that the 1-MPSG mediated intracellular ROS alterations in A549 and HaCaT cells. These results, proved by fluorescence spectroscopy, and flow cytometry, suggest that investigated Cu(I) compound may trigger apoptosis also through ROS generation.

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