Rapid in situ detection of alkaloids in plant tissue under ambient conditions using desorption electrospray ionization

The Analyst ◽  
2005 ◽  
Vol 130 (12) ◽  
pp. 1624 ◽  
Author(s):  
Nari Talaty ◽  
Zoltán Takáts ◽  
R. Graham Cooks
Keyword(s):  
Electrospray Ionization ◽  
Plant Tissue ◽  
Desorption Electrospray Ionization ◽  
Ambient Conditions ◽  
In Situ Detection

scholarly journals Nanopipette/Nanorod-Combined Quartz Tuning Fork–Atomic Force Microscope

Sensors ◽  
2019 ◽  
Vol 19 (8) ◽  
pp. 1794 ◽  
Author(s):  
Sangmin An ◽  
Wonho Jhe
Keyword(s):  
Atomic Force Microscope ◽  
Tuning Fork ◽  
High Sensitivity ◽  
Quartz Tuning Fork ◽  
Force Sensor ◽  
Au Nanoparticle ◽  
Ambient Conditions ◽  
Atomic Force ◽  
In Situ Detection

We introduce a nanopipette/quartz tuning fork (QTF)–atomic force microscope (AFM) for nanolithography and a nanorod/QTF–AFM for nanoscratching with in situ detection of shear dynamics during performance. Capillary-condensed nanoscale water meniscus-mediated and electric field-assisted small-volume liquid ejection and nanolithography in ambient conditions are performed at a low bias voltage (~10 V) via a nanopipette/QTF–AFM. We produce and analyze Au nanoparticle-aggregated nanowire by using nanomeniscus-based particle stacking via a nanopipette/QTF–AFM. In addition, we perform a nanoscratching technique using in situ detection of the mechanical interactions of shear dynamics via a nanorod/QTF–AFM with force sensor capability and high sensitivity.

scholarly journals Imaging the Unimaginable: Desorption Electrospray Ionization – Imaging Mass Spectrometry (DESI-IMS) in Natural Product Research

Planta Medica ◽  
2018 ◽  
Vol 84 (09/10) ◽  
pp. 584-593 ◽  
Author(s):  
Delphine Parrot ◽  
Stefano Papazian ◽  
Daniel Foil ◽  
Deniz Tasdemir
Keyword(s):  
Mass Spectrometry ◽  
Natural Product ◽  
Electrospray Ionization ◽  
Imaging Mass Spectrometry ◽  
Desorption Electrospray Ionization ◽  
Repeated Measurements ◽  
Chemical Information ◽  
Ambient Conditions ◽  
Chemical Screening ◽  
Natural Product Research

AbstractImaging mass spectrometry (IMS) has recently established itself in the field of “spatial metabolomics.” Merging the sensitivity and fast screening of high-throughput mass spectrometry with spatial and temporal chemical information, IMS visualizes the production, location, and distribution of metabolites in intact biological models. Since metabolite profiling and morphological features are combined in single images, IMS offers an unmatched chemical detail on complex biological and microbiological systems. Thus, IMS-type “spatial metabolomics” emerges as a powerful and complementary approach to genomics, transcriptomics, and classical metabolomics studies. In this review, we summarize the current state-of-the-art IMS methods with a strong focus on desorption electrospray ionization (DESI)-IMS. DESI-IMS utilizes the original principle of electrospray ionization, but in this case solvent droplets are rastered and desorbed directly on the sample surface. The rapid and minimally destructive DESI-IMS chemical screening is achieved at ambient conditions and enables the accurate view of molecules in tissues at the µm-scale resolution. DESI-IMS analysis does not require complex sample preparation and allows repeated measurements on samples from different biological sources, including microorganisms, plants, and animals. Thanks to its easy workflow and versatility, DESI-IMS has successfully been applied to many different research fields, such as clinical analysis, cancer research, environmental sciences, microbiology, chemical ecology, and drug discovery. Herein we discuss the present applications of DESI-IMS in natural product research.

In situ detection of γ-hydroxybutyrate and γ-butyrolactone in drinks by secondary electrospray ionization

2013 ◽  
Vol 5 (4) ◽  
pp. 844-850 ◽  
Author(s):  
Christian Berchtold ◽  
Stefan Schmid ◽  
Lukas Meier ◽  
Renato Zenobi
Keyword(s):  
Electrospray Ionization ◽  
Secondary Electrospray Ionization ◽  
In Situ Detection
Sign in / Sign up

Export Citation Format

Share Document