Mass Spectrometry Sampling Under Ambient Conditions with Desorption Electrospray Ionization

Science ◽  
2004 ◽  
Vol 306 (5695) ◽  
pp. 471-473 ◽  
Author(s):  
Z. Takats
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Desorption Electrospray Ionization ◽  
Ambient Conditions

scholarly journals Imaging the Unimaginable: Desorption Electrospray Ionization – Imaging Mass Spectrometry (DESI-IMS) in Natural Product Research

Planta Medica ◽  
2018 ◽  
Vol 84 (09/10) ◽  
pp. 584-593 ◽  
Author(s):  
Delphine Parrot ◽  
Stefano Papazian ◽  
Daniel Foil ◽  
Deniz Tasdemir
Keyword(s):  
Mass Spectrometry ◽  
Natural Product ◽  
Electrospray Ionization ◽  
Imaging Mass Spectrometry ◽  
Desorption Electrospray Ionization ◽  
Repeated Measurements ◽  
Chemical Information ◽  
Ambient Conditions ◽  
Chemical Screening ◽  
Natural Product Research

AbstractImaging mass spectrometry (IMS) has recently established itself in the field of “spatial metabolomics.” Merging the sensitivity and fast screening of high-throughput mass spectrometry with spatial and temporal chemical information, IMS visualizes the production, location, and distribution of metabolites in intact biological models. Since metabolite profiling and morphological features are combined in single images, IMS offers an unmatched chemical detail on complex biological and microbiological systems. Thus, IMS-type “spatial metabolomics” emerges as a powerful and complementary approach to genomics, transcriptomics, and classical metabolomics studies. In this review, we summarize the current state-of-the-art IMS methods with a strong focus on desorption electrospray ionization (DESI)-IMS. DESI-IMS utilizes the original principle of electrospray ionization, but in this case solvent droplets are rastered and desorbed directly on the sample surface. The rapid and minimally destructive DESI-IMS chemical screening is achieved at ambient conditions and enables the accurate view of molecules in tissues at the µm-scale resolution. DESI-IMS analysis does not require complex sample preparation and allows repeated measurements on samples from different biological sources, including microorganisms, plants, and animals. Thanks to its easy workflow and versatility, DESI-IMS has successfully been applied to many different research fields, such as clinical analysis, cancer research, environmental sciences, microbiology, chemical ecology, and drug discovery. Herein we discuss the present applications of DESI-IMS in natural product research.

Rapid detection of Fusarium wilt in basil (Ocimum sp.) leaves by desorption electrospray ionization mass spectrometry (DESI MS) imaging

RSC Advances ◽  
2015 ◽  
Vol 5 (62) ◽  
pp. 50512-50522 ◽  
Author(s):  
R. G. Hemalatha ◽  
Hemanta R. Naik ◽  
Vasundhara Mariappa ◽  
T. Pradeep
Keyword(s):  
Mass Spectrometry ◽  
Spatial Distribution ◽  
Electrospray Ionization ◽  
Electrospray Ionization Mass Spectrometry ◽  
Rapid Detection ◽  
Desorption Electrospray Ionization ◽  
Ionization Mass ◽  
Ambient Conditions ◽  
Ms Imaging ◽  
Desi Ms

A rapid method to unravel the spatial distribution ofFusarium/other pathogen-contamination in asymptomatic leaves under ambient conditions.

scholarly journals Accelerating Strain Engineering using Desorption Electrospray Ionization-Imaging Mass Spectrometry and Untargeted Molecular Analysis of Intact Microbial Colonies.

2021 ◽  
Author(s):  
Berkley M Ellis ◽  
Piyoosh Babele ◽  
Jody C May ◽  
Brian F Pfleger ◽  
Jamey D Young ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Imaging Mass Spectrometry ◽  
Desorption Electrospray Ionization ◽  
Strain Engineering ◽  
Specific Gene ◽  
Ambient Conditions ◽  
Secondary Products ◽  
Metabolic Phenotypes ◽  
Cell Factories

Progress in the fields of genomic and biologic sciences has yielded microbial bioprocesses for the advanced production of chemicals. While biomanufacturing has the potential to address global demands for renewable fuels and chemicals, engineering microbial cell factories that can compete with synthetic chemical processes remains a challenge. Optimizing strains for enhanced chemical production is no longer limited by reading and writing DNA, rather it is impeded by the lack of high-throughput platforms for characterizing the metabolic phenotypes resulting from specific gene editing events. To address this issue, we have developed a desorption electrospray ionization- imaging mass spectrometry (DESI-IMS) screening assay that is conducive to both multiplexed sampling and untargeted analyses. This technology bridges the gap between genomic and metabolomic timescales by simultaneously characterizing the chemical output of various engineered Escherichia coli strains rapidly and directly under ambient conditions. The developed method was used to phenotype four E. coli strains on the basis of measured metabolomes, which were validated via PCR genotyping. Untargeted DESI-IMS phenotyping suggests multiple strategies for future engineering which include: (i) relative amounts of specific biosynthetic products, (ii) identification of secondary products, and (iii) the metabolome of engineered organisms. In sum, we present a workflow to accelerate strain engineering by providing rapid, untargeted, and multiplexed analyses of microbial metabolic phenotypes.

Intraoperative assessment of isocitrate dehydrogenase mutation status in human gliomas using desorption electrospray ionization–mass spectrometry

2020 ◽  
Vol 132 (1) ◽  
pp. 180-187 ◽  
Author(s):  
Clint M. Alfaro ◽  
Valentina Pirro ◽  
Michael F. Keating ◽  
Eyas M. Hattab ◽  
R. Graham Cooks ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Isocitrate Dehydrogenase ◽  
Tumor Resection ◽  
Desorption Electrospray Ionization ◽  
Extent Of Resection ◽  
Ionization Mass ◽  
Idh Mutation ◽  
Mutation Status ◽  
Desi Ms

OBJECTIVEThe authors describe a rapid intraoperative ambient ionization mass spectrometry (MS) method for determining isocitrate dehydrogenase (IDH) mutation status from glioma tissue biopsies. This method offers new glioma management options and may impact extent of resection goals. Assessment of the IDH mutation is key for accurate glioma diagnosis, particularly for differentiating diffuse glioma from other neoplastic and reactive inflammatory conditions, a challenge for the standard intraoperative diagnostic consultation that relies solely on morphology.METHODSBanked glioma specimens (n = 37) were analyzed by desorption electrospray ionization–MS (DESI-MS) to develop a diagnostic method to detect the known altered oncometabolite in IDH-mutant gliomas, 2-hydroxyglutarate (2HG). The method was used intraoperatively to analyze tissue smears obtained from glioma patients undergoing resection and to rapidly diagnose IDH mutation status (< 5 minutes). Fifty-one tumor core biopsies from 25 patients (14 wild type [WT] and 11 mutant) were examined and data were analyzed using analysis of variance and receiver operating characteristic curve analysis.RESULTSThe optimized DESI-MS method discriminated between IDH-WT and IDH-mutant gliomas, with an average sensitivity and specificity of 100%. The average normalized DESI-MS 2HG signal was an order of magnitude higher in IDH-mutant glioma than in IDH-WT glioma. The DESI 2HG signal intensities correlated with independently measured 2HG concentrations (R2 = 0.98). In 1 case, an IDH1 R132H–mutant glioma was misdiagnosed as a demyelinating condition by frozen section histology during the intraoperative consultation, and no resection was performed pending the final pathology report. A second craniotomy and tumor resection was performed after the final pathology provided a diagnosis most consistent with an IDH-mutant glioblastoma. During the second craniotomy, high levels of 2HG in the tumor core biopsies were detected.CONCLUSIONSThis study demonstrates the capability to differentiate rapidly between IDH-mutant gliomas and IDH-WT conditions by DESI-MS during tumor resection. DESI-MS analysis of tissue smears is simple and can be easily integrated into the standard intraoperative pathology consultation. This approach may aid in solving differential diagnosis problems associated with low-grade gliomas and could influence intraoperative decisions regarding extent of resection, ultimately improving patient outcome. Research is ongoing to expand the patient cohort, systematically validate the DESI-MS method, and investigate the relationships between 2HG and tumor heterogeneity.

Mass Spectrometry for Proteomics and Recent Developments in ESI, MALDI and other Ionization Methodologies

2019 ◽  
Vol 16 (4) ◽  
pp. 267-276
Author(s):  
Qurat ul Ain Farooq ◽  
Noor ul Haq ◽  
Abdul Aziz ◽  
Sara Aimen ◽  
Muhammad Inam ul Haq
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
New Technologies ◽  
Protein Complexes ◽  
Imaging Techniques ◽  
Desorption Electrospray Ionization ◽  
High Throughput Analysis ◽  
Enhanced Sensitivity ◽  
Recent Developments ◽  
The Way

Background: Mass spectrometry is a tool used in analytical chemistry to identify components in a chemical compound and it is of tremendous importance in the field of biology for high throughput analysis of biomolecules, among which protein is of great interest. Objective: Advancement in proteomics based on mass spectrometry has led the way to quantify multiple protein complexes, and proteins interactions with DNA/RNA or other chemical compounds which is a breakthrough in the field of bioinformatics. Methods: Many new technologies have been introduced in electrospray ionization (ESI) and Matrixassisted Laser Desorption/Ionization (MALDI) techniques which have enhanced sensitivity, resolution and many other key features for the characterization of proteins. Results: The advent of ambient mass spectrometry and its different versions like Desorption Electrospray Ionization (DESI), DART and ELDI has brought a huge revolution in proteomics research. Different imaging techniques are also introduced in MS to map proteins and other significant biomolecules. These drastic developments have paved the way to analyze large proteins of >200kDa easily. Conclusion: Here, we discuss the recent advancement in mass spectrometry, which is of great importance and it could lead us to further deep analysis of the molecules from different perspectives and further advancement in these techniques will enable us to find better ways for prediction of molecules and their behavioral properties.

Visualizing mass transport in desorption electrospray ionization using time-of-flight secondary ion mass spectrometry: a look at the geometric configuration of the spray

The Analyst ◽  
2014 ◽  
Vol 139 (22) ◽  
pp. 5868-5878 ◽  
Author(s):  
Shin Muramoto
Keyword(s):  
Mass Spectrometry ◽  
Mass Transport ◽  
Electrospray Ionization ◽  
Secondary Ion Mass Spectrometry ◽  
Time Of Flight ◽  
Desorption Electrospray Ionization ◽  
Geometric Configuration ◽  
Ion Mass Spectrometry ◽  
Secondary Ion

The desorption profile of analyte molecules desorbed by desorption electrospray ionization was imaged and characterized using time-of-flight secondary ion mass spectrometry.

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