Selenium speciation analysis of selenium-enriched supplements by HPLC with ultrasonic nebulisation ICP-MS and electrospray MS/MS detection

2004 ◽  
Vol 19 (12) ◽  
pp. 1529-1538 ◽  
Author(s):  
Heidi Goenaga Infante ◽  
Gavin O’Connor ◽  
Margaret Rayman ◽  
Raimund Wahlen ◽  
John Entwisle ◽  
...  
Keyword(s):  
Speciation Analysis ◽  
Selenium Speciation ◽  
Ms Detection ◽  
Icp Ms

Analysis for selenium speciation in selenized yeast extracts by two-dimensional liquid chromatography with ICP-MS and electrospray MS-MS detection

2001 ◽  
Vol 16 (1) ◽  
pp. 68-73 ◽  
Author(s):  
Shona McSheehy ◽  
Paweł Pohl ◽  
Joanna Szpunar ◽  
Martine Potin-Gautier ◽  
Ryszard Łobiński
Keyword(s):  
Liquid Chromatography ◽  
Selenium Speciation ◽  
Two Dimensional ◽  
Ms Detection ◽  
Icp Ms ◽  
Selenized Yeast

Speciation analysis of nickel in the latex of a hyperaccumulating tree Sebertia acuminata by HPLC and CZE with ICP MS and electrospray MS-MS detection

2003 ◽  
Vol 18 (2) ◽  
pp. 120-127 ◽  
Author(s):  
Dirk Schaumlöffel ◽  
Laurent Ouerdane ◽  
Brice Bouyssiere ◽  
Ryszard Łobiński
Keyword(s):  
Speciation Analysis ◽  
Ms Detection ◽  
Icp Ms

Detection and characterization of artefact compounds during selenium speciation analysis in yeast by ICP-MS-assisted MALDI MS, oMALDI MS/MS and LC-ES-MS/MS

2006 ◽  
Vol 21 (7) ◽  
pp. 703-707 ◽  
Author(s):  
M. Dernovics ◽  
L. Ouerdane ◽  
L. Tastet ◽  
P. Giusti ◽  
H. Preud’homme ◽  
...  
Keyword(s):  
Speciation Analysis ◽  
Maldi Ms ◽  
Selenium Speciation ◽  
Icp Ms

scholarly journals Selenium speciation analysis of selenium-enriched food sprouts

2014 ◽  
pp. 23-28
Author(s):  
Éva Bódi ◽  
Dávid Andrási ◽  
Béla Kovács
Keyword(s):  
Citric Acid ◽  
Sample Preparation ◽  
Speciation Analysis ◽  
Selenium Speciation ◽  
Selenium Content ◽  
Selenium Species ◽  
Extraction Solvent ◽  
Icp Ms ◽  
Significant Difference ◽  
Total Selenium

In this present study, we prepared selenium-enriched pea and wheat sprouts. During our research we aimed not only to measure the total selenium content of the sprouts but to identify different selenium species. Scientifical researches show why the analytical examination of different selenium (Se) species is necessary: consumption of all kind of Se-species is useful for a person who suffers in selenium deficit, while there is significant difference between effects of different Se-species on person, in whose body the Se-level is just satisfactory. Biological availability, capitalization, accumulation, toxicity of Se-species are different, but the main difference was manifested in the anti-cancer effect of selenium. During our research selenium was used in form of sodium selenite and sodium selenate, the concentration of the solutions used for germination was 10 mg dm-3. Control treatment meant germination in distilled water. Total selenium content of sprout samples was measured after microwave digestion by inductively coupled plasma mass spectrometry (ICP-MS). Different extraction solvents were applied during sample preparation in order to separate different Se-species (0.1 M and 0.2 M HCl or 10 mM citric acid buffer). We wanted the following question to be answered: Which extraction solvent resulted the best extraction efficiency? Selenium speciation analysis of sprout sample extracts was performed by high performance liquid chromatography with anion exchange column, detection of selenium species was performed by ICP-MS. Evaluating our experimental results we have been found that significant amount of selenium of inorganic forms used during germination transformed into organic selenium compounds. There was difference between the amount of Se-species in pea and wheat sprouts and selenium uptake and repartition of selenium species were depended on Se-form used during germination. In addition the chromatogram analysis made us clear as well, that the citric acid solvent proved to be the most effective extraction solvent during sample preparation int he view of organic Se species.

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