Determination of the binding specificity of the 12S subunit of the transcarboxylase by saturation transfer difference NMR

2004 ◽  
Vol 2 (12) ◽  
pp. 1777 ◽  
Author(s):  
Claudia Peikert ◽  
Karsten Seeger ◽  
Rakesh Kumar Bhat ◽  
Stefan Berger
Keyword(s):  
Binding Specificity ◽  
Saturation Transfer ◽  
Saturation Transfer Difference

MEASUREMENT OF STEROID BINDING PROTEINS

1970 ◽  
Vol 65 (1_Suppl) ◽  
pp. S104-S121 ◽  
Author(s):  
E. E. Baulieu ◽  
J. P. Raynaud ◽  
E. Milgrom
Keyword(s):  
Kinetic Parameters ◽  
Binding Proteins ◽  
Binding Specificity ◽  
Binding Parameters ◽  
Target Organs ◽  
Biological Mixtures ◽  
Steroid Binding Proteins ◽  
Steroid Binding ◽  
Steroid Binding Protein

ABSTRACT A brief review of the characteristics of steroid binding proteins found in the plasma and in some target organs is presented, followed by some general remarks on binding »specificity« and binding parameters. Useful techniques for measuring binding parameters at equilibrium are reported, both those which keep the equilibrium intact and those which implicate its disruption. A concept is developed according to which the determination of a specific steroid binding protein is based on the »differential dissociation« of the several steroid binding complexes present in most biological mixtures. Methods which allow determination of the kinetic parameters of the binding systems are also presented. Various representations of the binding and therefore different modes of graphic representation and calculation are discussed, including the recent »proportion graph« method.

Synthetic peptide libraries in the determination of T cell epitopes and peptide binding specificity of class I molecules

1992 ◽  
Vol 22 (6) ◽  
pp. 1405-1412 ◽  
Author(s):  
Ton N. M. Schumacher ◽  
Grada M. Van Bleek ◽  
Marie-ThéRèSe Heemels ◽  
Karl Deres ◽  
Ka Wan Li ◽  
...  
Keyword(s):  
T Cell ◽  
Synthetic Peptide ◽  
Peptide Binding ◽  
Binding Specificity ◽  
Peptide Libraries ◽  
Class I ◽  
T Cell Epitopes ◽  
Peptide Binding Specificity
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